Experimental inoculation of oriental river prawn Macrobrachium nipponense with white spot syndrome virus (WSSV)

Zhao, Caiyuan; Fu, Hongtuo; Sun, Shengming; Qiao, Hui; Zhang, Wenyi; Jin, Shubo; Jiang, Sufei; Xiong, Yiwei; Gong, Yongsheng

doi:10.3354/dao03165

Cited by 20 publications

(15 citation statements)

References 75 publications

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“…WSSV is a member of genus Whispovirus, which belongs to the Nimaviridae family. WSSV can infect more than 93 species of shrimp and prawn [13], including Litopenaeus vannamei, Penaeus monodon, and M. japonicas [14]. The cumulative mortality of shrimp could approach 100% in 7-10 days after WSSV infection.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

Jing

Liu

et al. 2020

Fish & Shellfish Immunology

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L-type lectins (LTLs) belong to the lectin family and are characterized by a conserved structural motif in their carbohydrate recognition domain. LTLs are homologous to leguminous lectins. In this study, we identified and functionally characterized an LTL from kuruma shrimp Marsupenaeus japonicus. We designated this LTL as MjLTL2. MjLTL2 contains a signal peptide, a Lectin_leg domain, a coiled coil, and transmembrane domain. MjLTL2 is distributed in hemocytes, heart, hepatopancreas, gill, stomach, and intestine; higher expression levels are seen in hemocytes and the hepatopancreas than in other tissues. MjLTL2 was upregulated following challenge of shrimp with Vibrio anguillarum and white spot syndrome virus (WSSV). MjLTL2 can agglutinate several bacteria without Ca 2+ . In addition, MjLTL2 could bind to several Gram-positive and -negative bacteria by binding to their lipopolysaccharide and peptidoglycan. However, MjLTL2 could not enhance the clearance of V. anguillarum in vivo. In the presence of WSSV infection, MjLTL2 knockdown by RNA interference resulted in a 7day lower cumulative mortality of M. japonicus. Moreover, less VP19, VP24, VP26, and VP28 mRNAs were extracted from the hemocytes of MjLTL2 knockdown shrimp than from the control. These results suggest that MjLTL2 is involved in immune responses in shrimp.

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Section: Introductionmentioning

confidence: 99%

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

Jing

Liu

et al. 2020

Fish & Shellfish Immunology

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“…Further challenge studies showed M. rosenbergii could be infected by injection with WSSV stains Thai-1 and Viet, however, the infectious dose to reach 50% endpoint in M. rosenbergii needed 20-fold and 400-fold more than that in P. vannamei, respectively [22]. The half lethal dose (LD50) of WSSV to M. nipponense by injection was 10 (3.84±0.06) copies/g, which was about 1780-fold higher than that to P. vannamei at 10 (0.59±0.22) copies/g [23]. It was demonstrated that M. rosenbergii could clear injected infectious WSSV after 5 to 50 days post-injection [24].…”

Section: Discussionmentioning

confidence: 90%

First Description of a Natural Infection with Shrimp Hemocyte Iridescent Virus in Farmed Giant Freshwater Prawn, <em>Macrobrachium Rosenbergii</em>

Qiu¹,

Chen²,

Zhao³

et al. 2019

Preprint

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Macrobrachium rosenbergii is a valuable freshwater prawn in Asian aquaculture. In recent years, a new symptom that was generally called as ‘white head’ caused high mortality in M. rosenbergii farms in China. Samples of M. rosenbergii, M. nipponense, Procambarus clarkii, M. superbum, Penaeus vannamei, and Cladocera from a farm suffering from ‘white head’ in Jiangsu Province were collected and analyzed in this study. Pathogen detection showed that all samples were positive for Shrimp hemocyte iridescent virus (SHIV). Histopathological examination revealed dark eosinophilic inclusions and pyknosis in hematopoietic tissue, hepatopancreas and gills of M. rosenbergii and M. nipponense. Blue signals of in situ DIG-labeled LAMP (ISDL) appeared in hematopoietic tissue, hemocytes, hepatopancreatic sinus, and antennal gland. TEM of ultrathin sections showed a large number of SHIV particles with a mean diameter about 157.9 nm. The virogenic stromata and budding virions were observed in hematopoietic cells. Quantitative detection by TaqMan probe based real-time PCR of different tissues in natural infected M. rosenbergii showed that hematopoietic tissue contained the highest SHIV load with a relative abundance of (25.4±16.9)%. Hepatopancreas and muscle contained the lowest SHIV load with a relative abundance at (2.44±1.24)% and (2.44±2.16)%, respectively. Above results verified that SHIV is the pathogen causing ‘white head’ in M. rosenbergii, and M. nipponense and Pr. clarkii are also the susceptible species of SHIV.

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“…All of the experimental materials and methods are referenced from Zhao et al (2017) [44]. The injection positions were between the third and fourth abdominal segments.…”

Section: Methodsmentioning

confidence: 99%

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

Qiao

et al. 2018

IJMS

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Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin), and RPL18 (Ribosomal protein L18). The stability of each reference gene was evaluated by GeNorm, NormFinder, BestKeeper, and comparative ∆C t methods, and was comprehensively ranked using RefFinder. RPL18 was shown to be the most suitable reference gene for adult M. nipponense tissues, while EIF was the most stable in different ovarian and embryo stages and in white spot syndrome virus infection, and β-act was the most stable reference gene under hypoxia stress. The reliability of the rankings was confirmed by RNA interference experiments. To the best of our knowledge, this represents the first systematic analysis of reference genes for qPCR experiments in M. nipponense, and the results will provide invaluable information for future research in closely related crustaceans.

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Experimental inoculation of oriental river prawn Macrobrachium nipponense with white spot syndrome virus (WSSV)

Cited by 20 publications

References 75 publications

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

First Description of a Natural Infection with Shrimp Hemocyte Iridescent Virus in Farmed Giant Freshwater Prawn, <em>Macrobrachium Rosenbergii</em>

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

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