Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Brito, Nara Macêdo Botelho; Brito, Marcus Vinicius Henriques; Carvalho, Rita de Kássia Vidigal; Matos, Lia Tavares de Moura Brasil; Veloso, Thiago Sousa; Vasconcelos, Diego Matos de; Brito, Rosângela Baía

doi:10.1590/s0102-86502007000600014

Cited by 4 publications

(2 citation statements)

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“…This tumor has become one of the few experimental models available with high rate of proliferation, rapid development, rare inoculation failures, and spontaneous regression (Moraes et al, 2000;Shaughnessy et al, 1991;Silva et al, 2002;Simpkins et al, 1991). These characteristics make the Walker 256 tumor induction a widely used model to test new antitumor substances since resistance to therapies and the high toxicity of chemotherapy and radiotherapy treatments are a major public health challenge (Brito et al, 2007).…”

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confidence: 99%

Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine

et al. 2021

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Glutamine is often used to treat metabolic changes associated with anorexia-cachexia syndrome in patients with malignant neoplasms. Walker 256 tumor is an excellent model for studying these changes associated with cancer in different organs, including injuries in testicular functions. However, the effects of supplementing glutamine on testicular morphometry in this model have not yet been investigated. Thus, the objective of this study was to evaluate the effect of L-glutamine supplementation on testicular morphometry in rats transplanted with Walker 256 tumor cells. Forty puberty Wistar rats were divided into four groups: control without L-glutamine (C); control supplemented with L-glutamine (CG); inoculated with Walker 256 tumor cells (WT) and inoculated with Walker 256 tumor cells and supplemented with L-glutamine (WTG). The testicles were removed, weighed, fixed in Bouin, and included in paraffin for histomorphometric analysis. Walker 256 tumor caused quantitative changes in the tubular and intertubular compartments and tunica albuginea, with reductions in the percentages of lumen and tunica albuginea, number of Sertoli cells per gram of testis; number of Leydig cells; percentage of blood vessels and connective tissue in intertubule. However, glutamine supplementation prevented part of these changes caused by the tumor, presenting mainly a protective effect on the tunica albuginea and percentage of blood and lymph vessels in the intertubule. These results indicate the potential of L-glutamine was able to recover for testicular dysfunction associated with cancer.

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Section: Introductionmentioning

confidence: 99%

Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine

et al. 2021

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“…The induction model of Walker 256 carcinoma has been largely described in the literature. However, the only available model of Walker 256 carcinoma in the uterine cervix was described by Brito et al 5 , which can be used to test novel drugs with anti-tumoral effects.…”

Section: Introductionmentioning

confidence: 99%

The effect of copaiba balsam on Walker 256 carcinoma inoculated into the vagina and uterine cervix of female rats

Brito

Carvalho

et al. 2010

Acta Cir. Bras.

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Purpose: To verify the copaiba balsam (Copaifera officinalis) effect on Walker 256 carcinoma inoculated into vagina and uterine cervix of rats. Methods: Eighteen female Wistar rats weighing between 180-250g were used, distributed into 2 groups (GCop, GC). On the 1st day of the experiment, 0.3 ml of Walker 256 carcinoma (2x10 6 concentration) was inoculated in both groups; on the 3rd day of the experiment, it was given 4.8 ml/kg of distilled water to the GC group, and 4.8 ml/kg of copaiba balsam to the GCop group. On the 12th day, euthanasia was performed and the tumor was grafted, being weighted and verified its volume. The data were submitted to statistical analysis with ANOVA test. Results: It was observed that copaiba balsam presented a negative inhibitory potential of 70%. Conclusion:The copaiba balsam stimulated the tumor growth. Key words: Plants, Medicinal. Carcinoma 256, Walker. Uterine Cervical Neoplasms. Rats. RESUMOObjetivo: Verificar o efeito do óleo de copaíba da espécie Copaifera officinalis no carcinoma de Walker 256 inoculado em vagina e colo de útero de ratas. Métodos: Foram utilizadas 18 ratas da linhagem Wistar, pesando entre 180-250g, distribuídas em dois grupos (CCop, GC). No 1º dia de experimento, em ambos os grupos foi inoculado 0,3ml de tumor de Walker 256 na concentração de 2x10 6 ; no 3º dia após essa inoculação, foi iniciada a administração de água destilada na dose de 4,8 ml/kg ao GC, e copaíba na dose de 4,8 ml/kg ao GCop. No 12° dia foi realizada a eutanásia das ratas e ressecado o tumor, sendo este pesado e averiguado seu volume. Os dados obtidos foram submetidos à análise estatística pelo método ANOVA. Resultados: Observou-se que o óleo de copaíba apresentou um potencial inibitório negativo de 70%. Conclusão: O óleo de copaíba estimulou o crescimento tumoral. Descritores: Plantas Medicinais. Carcinoma 256 de Walker. Neoplasias do Colo do Útero. Ratos.

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The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

et al. 2009

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Purpose: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. Methods: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. Results: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. Conclusion: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass. Keywords: Ovariectomy. Cervix Uteri. Vagina. Carcinoma 256, Walker. Rats. RESUMOObjetivo: Verificar o efeito da ooforectomia à inoculação do tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 10 ratas Wistar, fêmeas, virgens, adultas, distribuídas em dois grupos de estudo com 05 animais cada: grupo tumor (GT): ratas inoculadas com tumor de Walker 256, e grupo Ooforectomia (GO): ratas ooforectomizadas e inoculadas com tumor de Walker 256. No dia anterior à inoculação vaginal do tumor, foram inoculados 0,3ml de ácido acético na vagina das ratas de ambos os grupos; no dia seguinte, foi realizada a escarificação da parede vaginal com uma escova de endocérvice e inoculado tumor de Walker 256. Após 12 dias, foi removido o tumor em bloco com vagina e cornos uterinos para análise macro e microscópica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença estatística entre os dois grupos; no entanto, notou-se que o comportamento de crescimento do tumor no grupo GO apresentou maior invasividade em extensão, comprometendo cornos uterinos. Conclusão: Observou-se que o grupo GO apresentou comportamento macroscópico diferente ao grupo GT, no entanto, em termos totais de massa tumoral ambos apresentam desenvolvimento similar. Descritores: Ovariectomia. Colo do Útero. Vagina. Carcinoma 256 Walker. Rats.

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Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Cited by 4 publications

References 8 publications

Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine

Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine

The effect of copaiba balsam on Walker 256 carcinoma inoculated into the vagina and uterine cervix of female rats

The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

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