Experimental infections of wild bank voles ( Myodes glareolus ) from nephropatia epidemica endemic and non-endemic regions revealed slight differences in Puumala virological course and immunological responses

Dubois, A.; Castel, Guillaume; Murri, Séverine; Pulido, Coralie; Pons, Jean-Baptiste; Benoit, Laure; Loiseau, Anne; Lakhdar, Latifa; Galan, Maxime; Charbonnel, Nathalie; Marianneau, Philippe

doi:10.1016/j.virusres.2017.04.004

Cited by 14 publications

(35 citation statements)

References 34 publications

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“…After 7 dpi, PUUV viral load decreased and persisted in bank voles while the bank vole serological response started to increase after two weeks of infection. The kinetics of PUUV replication and serological response observed in our study were congruent with the results obtained from bank voles from another French NE endemic (Jura) and non-endemic area (Ain) [ 14 , 19 ].…”

Section: Discussionsupporting

confidence: 90%

“…Over the last 10 years, a spatial expansion of NE cases outside of the endemic area has been observed in southern and western areas (National Reference Center for Hantavirus, [ 11 ]). We therefore previously proposed to discriminate NE endemic areas (lots of NE cases, for example, Ardennes [ 12 ]) and NE non-endemic areas (few or no cases of NE, for example, Loiret [ 13 ] and Ain [ 14 ]).…”

Section: Introductionmentioning

confidence: 99%

“…Other studies have focused on the role of biotic factors in shaping NE epidemiology and its variability in Europe and France [ 18 ]. They focused on the genetic characteristics of PUUV [ 13 ] or of the bank voles [ 14 , 19 , 20 , 21 , 22 ]. However, they scarcely considered the influence of PUUV/ M. glareolus interactions although these latter can strongly influence infection outcomes [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Experimental infections are relevant approaches to investigate the influence of host/pathogen interactions on eco-epidemiological processes, including host immune response, pathogen replication and excretion. They have rarely been developed to study reservoirs/hantaviruses interactions [ 25 ] and up to now, these experiments were conducted using either colonized bank voles [ 26 , 27 , 28 , 29 , 30 ] and/or cell adapted viral strains [ 14 , 25 , 31 ]. Only limited conclusions could be reached from these experiments, in particular because bank vole immunogenetic polymorphism [ 20 ] or PUUV genetic diversity [ 13 ] have previously been shown to influence the variability of bank vole/PUUV interaction outcomes.…”

Section: Introductionmentioning

confidence: 99%

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How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach

et al. 2020

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In Europe, Puumala virus (PUUV) is responsible for nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). Despite the presence of its reservoir, the bank vole, on most of French territory, the geographic distribution of NE cases is heterogeneous and NE endemic and non-endemic areas have been reported. In this study we analyzed whether bank vole-PUUV interactions could partly shape these epidemiological differences. We performed crossed-experimental infections using wild bank voles from French endemic (Ardennes) and non-endemic (Loiret) areas and two French PUUV strains isolated from these areas. The serological response and dynamics of PUUV infection were compared between the four cross-infection combinations. Due to logistical constraints, this study was based on a small number of animals. Based on this experimental design, we saw a stronger serological response and presence of PUUV in excretory organs (bladder) in bank voles infected with the PUUV endemic strain. Moreover, the within-host viral diversity in excretory organs seemed to be higher than in other non-excretory organs for the NE endemic cross-infection but not for the NE non-endemic cross-infection. Despite the small number of rodents included, our results showed that genetically different PUUV strains and in a lesser extent their interaction with sympatric bank voles, could affect virus replication and diversity. This could impact PUUV excretion/transmission between rodents and to humans and in turn at least partly shape NE epidemiology in France.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach

et al. 2020

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“…An alternative hypothesis states that spatial variation in the outcomes of M. glareolus / PUUV interactions may affect PUUV replication and excretion in the environment, which could ultimately shape PUUV distribution and nephropathia epidemica incidence in Europe (Guivier et al, 2014; Rohfritsch, Guivier, Galan, Chaval, & Charbonnel, 2013). Indeed, voles differ in their probability of being infected by PUUV (Kallio et al, 2006) and experimental infections have confirmed that the outcome of PUUV infection could vary between individuals (Dubois, Castel, et al, 2017; Hardestam et al, 2008). Furthermore, the genetic background of bank voles contributes to the variation in the response to infection (Charbonnel et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

Population genomics of bank vole populations reveals associations between immune related genes and the epidemiology of Puumala hantavirus in Sweden

Rohfritsch

Galan

Gautier

et al. 2017

Preprint

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Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden

Rohfritsch

Galan

Gautier

et al. 2018

Ecology and Evolution

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Natural reservoirs of zoonotic pathogens generally seem to be capable of tolerating infections. Tolerance and its underlying mechanisms remain difficult to assess using experiments or wildlife surveys. High‐throughput sequencing technologies give the opportunity to investigate the genetic bases of tolerance, and the variability of its mechanisms in natural populations. In particular, population genomics may provide preliminary insights into the genes shaping tolerance and potentially influencing epidemiological dynamics. Here, we addressed these questions in the bank vole Myodes glareolus, the specific asymptomatic reservoir host of Puumala hantavirus (PUUV), which causes nephropathia epidemica (NE) in humans. Despite the continuous spatial distribution of M. glareolus in Sweden, NE is endemic to the northern part of the country. Northern bank vole populations in Sweden might exhibit tolerance strategies as a result of coadaptation with PUUV. This may favor the circulation and maintenance of PUUV and lead to high spatial risk of NE in northern Sweden. We performed a genome‐scan study to detect signatures of selection potentially correlated with spatial variations in tolerance to PUUV. We analyzed six bank vole populations from Sweden, sampled from northern NE‐endemic to southern NE‐free areas. We combined candidate gene analyses (Tlr4, Tlr7, and Mx2 genes) and high‐throughput sequencing of restriction site‐associated DNA (RAD) markers. Outlier loci showed high levels of genetic differentiation and significant associations with environmental data including variations in the regional number of NE human cases. Among the 108 outliers that matched to mouse protein‐coding genes, 14 corresponded to immune‐related genes. The main biological pathways found to be significantly enriched corresponded to immune processes and responses to hantavirus, including the regulation of cytokine productions, TLR cascades, and IL‐7, VEGF, and JAK–STAT signaling. In the future, genome‐scan replicates and functional experimentations should enable to assess the role of these biological pathways in M. glareolus tolerance to PUUV.

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Experimental infections of wild bank voles ( Myodes glareolus ) from nephropatia epidemica endemic and non-endemic regions revealed slight differences in Puumala virological course and immunological responses

Cited by 14 publications

References 34 publications

How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach

How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach

Population genomics of bank vole populations reveals associations between immune related genes and the epidemiology of Puumala hantavirus in Sweden

Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden

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