Experimental infection with Escherichia coli O149:F4ac in weaned piglets

Jensen, Gerda M.; Frydendahl, Kai; Svendsen, Ove; Jørgensen, Claus B.; Cirera, Susanna; Fredholm, Merete; Nielsen, Jens Peter; Möller, Kristian

doi:10.1016/j.vetmic.2006.01.002

Cited by 57 publications

(56 citation statements)

References 25 publications

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“…In total, 20 Large White piglets (eight females, 12 males) were selected from a farm where ETEC infection has been frequently evidenced and on the basis of the polymorphism for the Mucin4 gene (Jensen et al, 2006), to obtain ETECsusceptible pigs.…”

Section: Animals Housing and Dietsmentioning

confidence: 99%

Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4

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et al. 2017

Animal

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The ability of live yeasts to modulate pig intestinal cell signals in response to infection with Escherichia coli F4ac (ETEC) has not been studied in-depth. The aim of this trial was to evaluate the effect of Saccharomyces cerevisiae CNCM I-4407 (Sc), supplied at different times, on the transcriptome profile of the jejunal mucosa of pigs 24 h after infection with ETEC. In total, 20 piglets selected to be ETEC-susceptible were weaned at 24 days of age (day 0) and allotted by litter to one of following groups: control (CO), CO + colistin (AB), CO + 5 × 10 10 colony-forming unit (CFU) Sc/kg feed, from day 0 (PR) and CO + 5 × 10 10 CFU Sc/kg feed from day 7 (CM). On day 7, the pigs were orally challenged with ETEC and were slaughtered 24 h later after blood sampling for haptoglobin (Hp) and C-reactive protein (CRP) determination. The jejunal mucosa was sampled (1) for morphometry; (2) for quantification of proliferation, apoptosis and zonula occludens (ZO-1); (3) to carry out the microarray analysis. A functional analysis was carried out using Gene Set Enrichment Analysis. The normalized enrichment score (NES) was calculated for each gene set, and statistical significance was defined when the False Discovery Rate % was <25 and P-values of NES were <0.05. The blood concentration of CRP and Hp, and the score for ZO-1 integrity on the jejunal villi did not differ between groups. The intestinal crypts were deeper in the AB (P = 0.05) and the yeast groups (P < 0.05) than in the CO group. Antibiotic treatment increased the number of mitotic cells in intestinal villi as compared with the control group (P < 0.05). The PR group tended to increase the mitotic cells in villi and crypts and tended to reduce the cells in apoptosis as compared with the CM group. The transcriptome profiles of the AB and PR groups were similar. In both groups, the gene sets involved in mitosis and in mitochondria development ranked the highest, whereas in the CO group, the gene sets related to cell junction and anion channels were affected. In the CM group, the gene sets linked to the metabolic process, and transcription ranked the highest; a gene set linked with a negative effect on growth was also affected. In conclusion, the constant supplementation in the feed with the strain of yeast tested was effective in counteracting the detrimental effect of ETEC infection in susceptible pigs limits the early activation of the gene sets related to the impairment of the jejunal mucosa.

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Section: Animals Housing and Dietsmentioning

confidence: 99%

Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4

Trevisi

Latorre

Priori

et al. 2017

Animal

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“…To establish experimental ETEC infection in pigs, prior oral administration of sodium bicarbonate is often required to address the low pH conditions in the stomach, and florfenicol is also administered prior to ETEC challenge to reduce competing intestinal flora, which may help the ETEC to survive and colonize [4,6,7]. However, we did not pretreat the pigs with either sodium bicarbonate or florfenicol, in order to evaluate the effectiveness of the pulverized phage under field conditions.…”

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confidence: 99%

Effect of Bacteriophage in Enterotoxigenic <i>Escherichia coli</i> (ETEC) Infected Pigs

Bin

Yoo

Lee

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage CJ12 in ETEC infected pigs. Phage was mixed with feed at a ratio of 1:1,000 (0.1%). One week after initially providing phage mixed feed, pigs were challenged orally with 10 11 CFU of ETEC and body weight, diarrhea score, bacterial CFU and phage PFU in the feces were measured. Pigs of phage treated groups C (10 6 PFU/g) and D (10 8 PFU/g) showed more resistance to diarrhea due to ETEC infection compared to positive control group B on the third day after the initial challenge. Moreover, during the quantitation of ETEC in feces, both groups C and D showed approximately 63.92 and 60.73% reduced ETEC compared to positive control group B. Phages were successfully isolated from feces in both groups C and D during the experiment without any adverse effects, suggesting the possibility of using CJ12 as a feed additive.

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“…Even though diarrhoea of varying intensities was observed in a majority of piglets, it was largely mild. In the study of Jensen et al (2006), an infection was induced in piglets using the strain ETEC O149:F4ac and diarrhoea developed in most of them. Nevertheless, they failed to induce haemorrhagic gastroenteritis.…”

Section: Discussionmentioning

confidence: 99%

“…However, they only found diarrhoea and fluid distension of small and large intestines in dead piglets at autopsy. Jensen et al (2006) infected F4ac/ab susceptible piglets after weaning with strains ETEC O149:F4 and described occurrence of diarrhoea in 74% of them. However, they did not mention any case of haemorrhagic gastroenteritis.…”

Section: Discussionmentioning

confidence: 99%

Experimental infection of weaned piglets with enterotoxigenic Escherichia coli O149:F4

et al. 2011

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EnterotoxigenicEscherichia coliis an important enteric pathogen causing post-weaning diarrhoea in piglets. Enterotoxins ofE. coliinduce the release of fluid into the intestines without apparent inflammation. Some serotypes ofE. coli, especially O149:F4 can often be identified in association with haemorrhagic gastroenteritis. In this study we infected the weaned piglets (n = 53) by oral administration of enterotoxigenicE. coliO149:F4 strains isolated from piglets suffering from haemorrhagic gastroenteritis. The clinical course of infection and shedding of the challengeE. colistrain in the faeces of infected piglets was monitored for 14 days. The challenge strain soon outnumbered the otherE. colitypes in the intestines of most piglets. Diarrhoea developed in the majority of piglets and its severity varied. Severe diarrhoea was observed in 10% of the piglets but only one piglet died due to dehydration. No inflammatory lesions were detected in the intestines of the dead piglet and the other euthanized piglets. We assume that development of haemorrhagic gastroenteritis depends on the involvement of other factors that need to be identified.E. coliO149:F4 are only one of the causative factors of haemorrhagic gastroenteritis in piglets after weaning.

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Experimental infection with Escherichia coli O149:F4ac in weaned piglets

Cited by 57 publications

References 25 publications

Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4

Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4

Effect of Bacteriophage in Enterotoxigenic <i>Escherichia coli</i> (ETEC) Infected Pigs

Experimental infection of weaned piglets with enterotoxigenic Escherichia coli O149:F4

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