Experimental Infection with and Maintenance of Cell Fusing Agent Virus (Flavivirus) in Aedes aegypti

Contreras-Gutierrez, María Angélica; Guzmán, Hilda; Thangamani, Saravanan; Vasilakis, Nikos; Tesh, Robert B.

doi:10.4269/ajtmh.16-0987

Cited by 28 publications

(32 citation statements)

References 31 publications

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“…That ISVs can affect transmission of medically important arboviruses is now considered likely 4 , 6 , 54 , 55 . That laboratory vector competence studies can be inconsistent and may not reliably reflect transmission in the field is becoming increasingly recognized 56 .…”

Section: Discussionmentioning

confidence: 99%

Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok

Zakrzewski

Rašić

Darbro

et al. 2018

Sci Rep

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Medically important arboviruses such as dengue, Zika, and chikungunya viruses are primarily transmitted by the globally distributed mosquito Aedes aegypti. Increasing evidence suggests that transmission can be influenced by mosquito viromes. Herein RNA-Seq was used to characterize RNA metaviromes of wild-caught Ae. aegypti from Bangkok (Thailand) and from Cairns (Australia). The two mosquito populations showed a high degree of similarity in their viromes. BLAST searches of assembled contigs suggest up to 27 insect-specific viruses may infect Ae. aegypti, with up to 23 of these currently uncharacterized and up to 16 infecting mosquitoes from both Cairns and Bangkok. Three characterized viruses dominated, Phasi Charoen-like virus, Humaita-Tubiacanga virus and Cell fusing agent virus, and comparisons with other available RNA-Seq datasets suggested infection levels with these viruses may vary in laboratory-reared mosquitoes. As expected, mosquitoes from Bangkok showed higher mitochondrial diversity and carried alleles associated with knock-down resistance to pyrethroids. Blood meal reads primarily mapped to human genes, with a small number also showing homology with rat/mouse and dog genes. These results highlight the wide spectrum of data that can be obtained from such RNA-Seq analyses, and suggests differing viromes may need to be considered in arbovirus vector competence studies.

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Section: Discussionmentioning

confidence: 99%

Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok

Zakrzewski

Rašić

Darbro

et al. 2018

Sci Rep

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“…There is growing evidence that the maintenance of insect-specific viruses within mosquito populations is via vertical transmission [ 12 , 13 , 14 , 15 ]. Indeed, the detection of mesoniviruses in adult male and female mosquitoes supports the hypothesis that mesoniviruses are transmitted from the female mosquito to her progeny [ 16 , 17 ], or via sexual transmission.…”

Section: Introductionmentioning

confidence: 99%

Genetic, Morphological and Antigenic Relationships between Mesonivirus Isolates from Australian Mosquitoes and Evidence for Their Horizontal Transmission

Newton

Colmant

O’Brien

et al. 2020

Viruses

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The Mesoniviridae are a newly assigned family of viruses in the order Nidovirales. Unlike other nidoviruses, which include the Coronaviridae, mesoniviruses are restricted to mosquito hosts and do not infect vertebrate cells. To date there is little information on the morphological and antigenic characteristics of this new group of viruses and a dearth of mesonivirus-specific research tools. In this study we determined the genetic relationships of recent Australian isolates of Alphamesonivirus 4 (Casuarina virus—CASV) and Alphamesonivirus 1 (Nam Dinh virus—NDiV), obtained from multiple mosquito species. Australian isolates of NDiV showed high-level similarity to the prototype NDiV isolate from Vietnam (99% nucleotide (nt) and amino acid (aa) identity). Isolates of CASV from Central Queensland were genetically very similar to the prototype virus from Darwin (95–96% nt and 91–92% aa identity). Electron microscopy studies demonstrated that virion diameter (≈80 nm) and spike length (≈10 nm) were similar for both viruses. Monoclonal antibodies specific to CASV and NDiV revealed a close antigenic relationship between the two viruses with 13/34 mAbs recognising both viruses. We also detected NDiV RNA on honey-soaked nucleic acid preservation cards fed on by wild mosquitoes supporting a possible mechanism of horizontal transmission between insects in nature.

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“…CFAV has been shown to be vertically transmitted in Ae. aegypti lab colonies suggesting the possibility of using CFAVs and closely related ISFs for control of medically important arboviruses 49 . The presence of insect-specific viruses in Ae.…”

Section: Discussionmentioning

confidence: 99%

Detection of a novel insect specific flavivirus across ecologically diverse populations of Aedes aegypti on the Caribbean Island of Saint Lucia

Jeffries

White

Wilson

et al. 2018

Preprint

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15 Outbreaks of mosquito-borne arboviral diseases including dengue virus (DENV), Zika virus (ZIKV), yellow 16 fever virus (YFV) and chikungunya virus (CHIKV) have recently occurred in the Caribbean. The 17 geographical range of the principle vectors responsible for transmission, Aedes (Ae.) aegypti and Ae.18 albopictus is increasing and greater mosquito surveillance is needed in the Caribbean given international 19 tourism is so prominent. The island of Saint Lucia has seen outbreaks of DENV and CHIKV in the past 20 five years but vector surveillance has been limited with the last studies dating back to the late 1970s. 21Natural disasters have changed the landscape of Saint Lucia and the island has gone through significant 22 urbanisation. In this study, we conducted an entomological survey of Ae. aegypti and Ae. albopictus 23 distribution across the island and analysed environmental parameters associated with the presence of 24 these species. Although we collected Ae. aegypti across a range of sites across the island, no Ae. 25 albopictus were collected despite traps being placed in diverse ecological settings. The number of Ae. 26aegypti collected was significantly associated with higher elevation and semi-urban settings yielded 27 female mosquito counts per trap-day that were 5-fold lower than urban settings. Screening for 28 arboviruses revealed a high prevalence of a novel insect-specific flavivirus closely related to cell fusing 29 agent virus (CFAV). We discuss the implications that natural disasters, water storage and lack of 30 mosquito surveillance have on arboviral outbreaks in Saint Lucia and implications for insect only 31 flaviviruses on surveillance and detection of pathogenic flaviviruses.32 33 34 35 36 37 38 4 . 57 Outbreaks of arboviral diseases including DENV 9 , YFV 7 , CHIKV 10 and ZIKV 11 have recently occurred in 58 the Caribbean. The possibility of additional recent arbovirus transmission in the Caribbean must be 59 considered given some infections result in nearly indistinguishable clinical symptoms. For example, 60 Mayaro virus (MAYV) is an alphavirus closely related to CHIKV and has resulted in sporadic outbreaks in 61 South America 12 . MAYV transmission is restricted to South and Central America where it is thought that 62 non-human primates act as reservoir hosts and Haemogogus mosquitoes (eg. H. janthinomys) found in 63 sylvatic jungle environments are responsible for human cases. Although human cases are strongly 64 correlated with exposure to forest environments, urban transmission of MAYV must be considered given 65 the association of cases and major cities infested with Ae. aegypti 13 . As the Caribbean is a destination 66 for many international tourists, surveillance is needed for individual Caribbean islands to determine the 67 risk of facilitating the spread of arboviral diseases. In particular, arboviruses transmitted by Ae. aegypti 68 are considered important given that prevention predominantly relies on mosquito vector control. Ae. 69 aegypti was first identified in the Ca...

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Experimental Infection with and Maintenance of Cell Fusing Agent Virus (Flavivirus) in Aedes aegypti

Cited by 28 publications

References 31 publications

Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok

Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok

Genetic, Morphological and Antigenic Relationships between Mesonivirus Isolates from Australian Mosquitoes and Evidence for Their Horizontal Transmission

Detection of a novel insect specific flavivirus across ecologically diverse populations of Aedes aegypti on the Caribbean Island of Saint Lucia

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