Experimental Infection of Specific-Pathogen-Free Chickens with Serotype-1 Fowl Adenovirus Isolated from a Broiler Chicken with Gizzard Erosions

Okuda, Yo; Ono, Masaaki; Yazawa, Shigeto; Shibata, Isao; Sato, Shizuo

doi:10.2307/1593007

Cited by 32 publications

(41 citation statements)

References 14 publications

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“…Inclusion body hepatitis (IBH) has been occurring as an economically important and reemerging disease of broiler breeder and broiler chickens in several countries (9,24,(29)(30)(31)(32). Although the IBH outbreaks are usually associated with serotype 6, 7, and 8, the viruses associated with outbreaks in Iran were adenovirus serotype 11, based on hexon gene sequencing of the PCR products (20).…”

Section: Discussionmentioning

confidence: 99%

“…For histopathological examination, livers from the suspected chickens were fixed in 10% buffered formalin, embedded in paraffin, sectioned at 4 μm, stained with hematoxylin and eosin and examined by light microscopy (24). DNA extraction DNA was extracted directly from the 20 infected liver tissues (10 samples from each farm according to the previously described method (22).…”

Section: Histopathological Examinationmentioning

confidence: 99%

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Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

Hosseini¹,

Morshed²

2012

Iran J Virol

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Background and Aims: Inclusion body hepatitis (IBH) is one of the most important reemerging diseases in many countries with intensive poultry industries. In Iran, the etiological agent of IBH (fowl adenovirus) has not been yet confirmed. The aim of this study was the molecular detection and identification of fowl adenovirus involving IBH in chicken flocks in Iran. Materials and Methods: Polymerase chain reaction (PCR) and sequence analysis of L1 hexon gene was utilized to detect and to determinate the genotypes of Fowl adenovirus (FAdV) in broiler breeder flocks. Histopathological sections were prepared and examined. Results: FAdVs were detected in livers. Based on sequencing analysis of the hexon gene, they were genetically related to FAdV-11, a member of the fowl adenovirus D species, with 98% homology to Korean strain in 2011. Histological examination revealed necrotizing hepatitis with basophilic intranuclear inclusion bodies in the hepatocytes. Conclusion: This study provided evidence for the role of fowl adenoviruses as agents, causing this clinical disease in Iran and indicated the importance of accurate diagnosis and prevention in the meat-type flocks.

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Section: Discussionmentioning

confidence: 99%

Section: Histopathological Examinationmentioning

confidence: 99%

Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

Hosseini¹,

Morshed²

2012

Iran J Virol

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“…Virus isolation: Virus isolation was attempted from the gizzard, liver, pancreas, and rectum (including feces) according to a method described previously [11]. Briefly, 10% tissue homogenates were inoculated onto CK cell cultures and the cultures were observed in order to detect any…”

Section: Methodsmentioning

confidence: 99%

“…Recently, serotype 8 FAV was isolated from gizzards exhibiting gizzard erosion in slaughterhouses [14]. In pervious reports, however, gizzard erosion was reproduced in SPF white leghorn chickens [9,11] and commercial broiler chickens [12] inoculated with serotype 1 FAV isolated from the gizzards of commercial broiler chickens exhibiting gizzard erosion. Moreover, most cases of gizzard erosion that have occurred frequently in slaughterhouses in Japan were caused by serotype 1 FAV infection [14].…”

mentioning

confidence: 99%

“…Necropsies were scheduled on days 3, 5, 7, and 10 PI and dead chickens and euthanized moribund chickens were necropsied immediately. One to 2 chickens in the control group were killed and necropsied on days 3, 4, 5, 7, and 10 PI.Virus isolation: Virus isolation was attempted from the gizzard, liver, pancreas, and rectum (including feces) according to a method described previously [11]. Briefly, 10% tissue homogenates were inoculated onto CK cell cultures and the cultures were observed in order to detect any…”

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confidence: 99%

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Pathogenicity of Serotype 8 Fowl Adenovirus Isolated from Gizzard Erosions of Slaughtered Broiler Chickens

Okuda

Ono

Shibata

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The pathogenicity of serotype 8 fowl adenovirus (FAV), isolated from gizzard erosions of slaughtered broiler chickens, was investigated. In experiment 1, 29 5-day-old specific-pathogen-free (SPF) chickens were inoculated with the isolates of serotype 8 FAV, M013 (group 1) or G0054 (group 2) strain, via an oral route. There were no clinical signs in any of chickens after inoculation, and mild gizzard erosions were observed macroscopically and microscopically in three inoculated chickens of group 2. FAV was recovered f rom gizzards and rectums but was not recovered from pancreas and livers from chickens in both inoculated groups. In experiment 2, 27 1-day-old SPF chickens were inoculated with the G0054 strain by intramuscular route. Five, 6, and 3 inoculated chickens died on days 3, 4, and 5 postinoculation (PI), respectively. Four, 3, 1, and 1 inoculated chickens became moribund with severe clinical sign s such as ruffled feathers, severe depression and closed eyes from days 3 to 6 PI, respectively. Macroscopically, the common characterist ic of the gross lesions of dead chickens and euthanized moribund chickens was discoloration of liver. FAV was recovered from the gizzard, liver, pancreas and rectum. Virus titers in the liver and pancreas were high until day 6 PI. Histologically, necrotizing hepatitis an d pancreatitis with intranuclear inclusion bodies were observed in the inoculated chickens. These results indicate that some strains of seroty pe 8 FAV are able to reproduce not only gizzard erosion by oral inoculation but inclusion body hepatitis (IBH) by intramuscular inoculation. KEY WORDS: gizzard erosion, inclusion body hepatitis, serotype 8 fowl adenovirus.J. Vet. Med. Sci. 66(12): 1561-1566, 2004 The pathogenicity of serotype 8 fowl adenoviruis (FAV) has been reported in field cases of inclusion body hepatitis (IBH) in chickens [7] as well as in experimental reproduction of IBH in specific-pathogen-free (SPF) chickens [6]. Recently, serotype 8 FAV was isolated from gizzards exhibiting gizzard erosion in slaughterhouses [14]. In pervious reports, however, gizzard erosion was reproduced in SPF white leghorn chickens [9,11] and commercial broiler chickens [12] inoculated with serotype 1 FAV isolated from the gizzards of commercial broiler chickens exhibiting gizzard erosion. Moreover, most cases of gizzard erosion that have occurred frequently in slaughterhouses in Japan were caused by serotype 1 FAV infection [14]. Therefore, it was not clear whether or not serotype 8 FAV was the cause of gizzard erosion.The purpose of this study was to investigate the pathogenicity of serotype 8 FAV isolated from eroded gizzard by inoculating SPF chickens with the isolates via oral and intramuscluar routes. MATERIALS AND METHODSVirus: Two isolates of FAV, isolated from gizzard erosions of slaughtered broiler chickens, were cloned three times by means of the terminal dilution method and designated as the M013 and G0054 strains. These strains were propagated in primary chicken kidney (CK) cells and used for chicke...

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Adenovirus Infections

Fitzgerald¹,

Rautenschlein²,

Mahsoub³

et al. 2019

Diseases of Poultry

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Experimental Infection of Specific-Pathogen-Free Chickens with Serotype-1 Fowl Adenovirus Isolated from a Broiler Chicken with Gizzard Erosions

Cited by 32 publications

References 14 publications

Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

Pathogenicity of Serotype 8 Fowl Adenovirus Isolated from Gizzard Erosions of Slaughtered Broiler Chickens

Adenovirus Infections

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