Experimental infection of rabbits with bovine herpesvirus-4: Acute and persistent infection

Osorio, Fernando A.; Reed, David E.; Rock, Daniel L.

doi:10.1016/0378-1135(82)90045-1

Cited by 74 publications

(95 citation statements)

References 16 publications

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“…Our results are consistent with the findings reported in previous papers (Osorio and Reed, 1983;Osorio et al, 1985;Lopez et al, 1996;Donofrio and van Santen, 2001) which indicated that macrophage-like PBLs must be the main reservoir of BoHV-4. We also found a correlation with our former experiment on PCR detection of BoHV-4 DNA from the serum of experimentally inoculated rabbits and calves (Egyed and Bartha, 1998).…”

Section: Discussionsupporting

confidence: 94%

“…The rabbit has been found a suitable and sensitive laboratory animal model for in vivo experimental studies of BoHV-4 infections (Osorio et al, 1982;Naeem et al, 1990;Naeem et al, 1991). Ten four-month-old New Zealand White rabbits were used in the experiment.…”

Section: Cell Line and Virus Strainmentioning

confidence: 99%

“…BoHV-4 is usually detected and isolated from peripheral blood leukocytes (PBLs), lymph nodes and spleen of rabbits (Naeem et al, 1990;Naeem et al, 1991) and cattle (Osorio and Reed, 1983;Egyed et al, 1996), although the exact cell tropism of the virus has never been identified in vivo. Cell separation studies indicated that a non-T, non-B cell population of bovine spleen harboured the BoHV-4 genome (Osorio et al, 1985).…”

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confidence: 99%

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Detection of bovine herpesvirus 4 in cd11b+ leukocytes of experimentally infected rabbits

Fábián

Ivanics

Terényi

et al. 2005

Acta Veterinaria Hungarica

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The presence and numbers of bovine herpesvirus 4 (BoHV-4) infected CD11b+ leukocytes were investigated during experimental infections of New Zealand White rabbits by Fluorescence Activated Cell Sorter (FACS) analysis. Peripheral blood leukocytes (PBL) were collected every second day, and the cells were stained with phycoerythrin-labelled CD11b-specific mouse monoclonal antibody and fluorescein-conjugated bovine herpesvirus 4-specific mouse monoclonal antibody. The numbers of double-stained cells from PBLs of the control and inoculated groups were measured and compared in FACS TREK analyser. Double-stained cells were detected in the virus-inoculated group on postinoculation days (PID) 2-5 and 9-12. The results indicated that CD11b+ PBLs were permissive for BoHV-4 infection, and are probably the main reservoir of the virus during the latent period. The data did not indicate production of infectious viral particles, but virus-specific proteins were expressed on the surface of CD11b+ cells. The two waves of double-stained cells gave similar results to the PCR assays from serum samples, which showed the presence of viral DNA in the serum on the same days when virus-infected CD11b cells were also present. Productive BoHV-4 infection of mast cells or undifferentiated leukocytes in the bone marrow and the antiviral immune response might be responsible for this periodic appearance of the virus in CD11b+ PBLs and in the serum. The paper provides evidence that CD11b+ PBLs are the main target cell populations in the blood for BoHV-4.

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Section: Discussionsupporting

confidence: 94%

Section: Cell Line and Virus Strainmentioning

confidence: 99%

mentioning

confidence: 99%

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Detection of bovine herpesvirus 4 in cd11b+ leukocytes of experimentally infected rabbits

Fábián

Ivanics

Terényi

et al. 2005

Acta Veterinaria Hungarica

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“…3,12,13 Variation in the pathogenic potential of BHV-4 isolates may be present, 15 and studies on BHV-4 isolates indicate that only certain isolates of BHV-4 have the potential to cause clinically identifiable infection in natural or experimental hosts. 4,5,15,17 BHV-4 induces neutralizing antibodies of low avidity that are barely detected by standard serum neutralization, but detection is enhanced with the addition of complement. 6,7,10,18 Because the detection of BHV-4 infection or exposure is difficult, a dot immunobing assay (DIA) antibody detection method was developed, and sensitivity was compared with that of a complement-dependent virus neutralization (VN) test.…”

Section: Introductionmentioning

confidence: 99%

Dot Immunobinding Assay for Detection of Bovine Herpesvirus 4 Antibodies in Rabbits

et al. 1999

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Abstract.A dot immunobinding assay (DIA) was used for the detection of antibody against bovine herpesvirus 4 (BHV-4) in experimentally infected specific-pathogen-free male and female rabbits. A semipurified virus preparation was used as the antigen, and protein A/G-horseradish-peroxidase conjugate and diaminobenzidine tetrahydrochloride were used as the detection system. Results of the DIA procedure on serum samples of experimentally infected male and female rabbits were compared with those of a complement-dependent virus neutralization (VN) test. None of the tested sera (0/60 samples) from either male or female rabbits were positive by the complement-dependent VN test. Results of the DIA procedure for the same tested sera were positive in 35 of 60 samples (58%) from BHV-4 infected rabbits, indicating higher sensitivity of DIA procedure as compared with the complement-dependent VN test.Bovine herpesvirus 4 (BHV-4) is one of the members of bovine herpesviruses, and is grouped in the family Herpesviridae, subfamily Gammaherpesvirinae. 8,11 A number of BHV-4 isolates have been reported from cattle with epididymitis, 21 vaginitis, 1 and abortion. 3,12,13 Variation in the pathogenic potential of BHV-4 isolates may be present, 15 and studies on BHV-4 isolates indicate that only certain isolates of BHV-4 have the potential to cause clinically identifiable infection in natural or experimental hosts. 4,5,15,17 BHV-4 induces neutralizing antibodies of low avidity that are barely detected by standard serum neutralization, but detection is enhanced with the addition of complement. 6,7,10,18 Because the detection of BHV-4 infection or exposure is difficult, a dot immunobing assay (DIA) antibody detection method was developed, and sensitivity was compared with that of a complement-dependent virus neutralization (VN) test. Materials and methodsSerum samples. Sixty serum samples were collected at various postinfection (PI) intervals from 4 groups of experimentally infected specific-pathogen-free SPF male and female Dutch-belted rabbits. Treatment, group design, and intervals of euthanasia of both male and female rabbits are given in Tables 1 and 2.Viral antigens. Strain 87-8363 of BHV-4 10 was propagated in bovine turbinate (BT) cell line a using Eagle's minimal Received for publication March 11, 1998. essential medium (MEM) containing 10% fetal bovine serum and antibiotics. After 90% cytopathic effect (CPE) was detected, the cell suspension was frozen and thawed 3 times (Ϫ70 C/37 C). The cells then were pelleted at 1,000 ϫ g for 20 min. The pellet was sonicated b for 15 sec with a 20-kHz microprobe at 75 watts of power and clarified by centrifugation at 1,000 ϫ g for 20 min. The supernatant was centrifuged for 2 hr at 44,000 ϫ g. c The virus pellet was resuspended in 0.1% bovine serum albumin (BSA) in Tris-buffered saline (TBS) (10 mM Tris, 150 mM NaCl, pH 7.6). Virus-free cell cultures, processed and treated the same way as the virus stock, were used as noninfected controls. Processed viral antigens and noninfected control...

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“…Similar to other herpesviruses, BHV-4 establishes a life-long latent infection in cattle after natural infection (Castrucci et al, 1991). Experimental inoculation of both cattle and rabbits with BHV-4 has been shown to produce persistent infection in these animals (Osorio et al, 1982;Osorio and Reed, 1983;Naeem et al, 1990;Naeem et al, 1991). The pathogenic effects of BHV-4 or other herpesviruses on the arteries of rabbits have not been reported, however.…”

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confidence: 99%

Experimental Infection with Bovine Herpesvirus-4 Enhances Atherosclerotic Process in Rabbits

Lin

Jiang

Eng

et al. 2000

Laboratory Investigation

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SUMMARY:An association of herpesvirus and atherosclerosis has been suggested by seroepidemiologic studies and detection of the virus in arterial tissues. To facilitate the studies of the pathogenic role of herpesvirus in atherosclerosis, we established a rabbit model of atherosclerosis with bovine herpesvirus type-4 (BHV-4). Forty New Zealand White rabbits were randomly divided into six groups. Groups 1, 2, and 3 were inoculated iv with BHV-4 and control Groups 4, 5, and 6 with normal saline. Groups 1 and 4 were fed a regular diet throughout the experiment; Groups 2 and 5 were fed a diet supplemented with 2% cholesterol for 3 weeks starting at 3 weeks postinoculation; and Groups 3 and 6 with a diet supplemented with 2% cholesterol for 6 weeks starting at 3 days postinoculation. Extensive atherosclerotic lesions in Groups 2, 3, and 6, and small lesions in two rabbits in Group 1 were observed, but no obvious lesions were observed in Groups 4 and 5. BHV-4 DNA was demonstrated by polymerase chain reaction and liquid hybridization in aortic sections, various tissue samples, and peripheral blood mononuclear cells of all infected rabbits. Our studies demonstrated that BHV-4 can accelerate the atherosclerotic process in rabbits, and that experimental infection of rabbits with BHV-4 can be a useful atherosclerosis model. (Lab Invest 2000, 80:3-11).

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Experimental infection of rabbits with bovine herpesvirus-4: Acute and persistent infection

Cited by 74 publications

References 16 publications

Detection of bovine herpesvirus 4 in cd11b+ leukocytes of experimentally infected rabbits

Detection of bovine herpesvirus 4 in cd11b+ leukocytes of experimentally infected rabbits

Dot Immunobinding Assay for Detection of Bovine Herpesvirus 4 Antibodies in Rabbits

Experimental Infection with Bovine Herpesvirus-4 Enhances Atherosclerotic Process in Rabbits

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