Experimental Infection of Cattle With a Feline Isolate of Tritrichomonas Foetus

Stockdale, Heather D.; Rodning, Soren P.; Givens, M. Daniel; Carpenter, David M.; Lenz, Stephen D.; Spencer, Jennifer; Dykstra, Christine C.; Lindsay, David S.; Blagburn, Byron L.

doi:10.1645/ge-1305.1

Cited by 46 publications

(36 citation statements)

References 17 publications

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“…Furthermore, a recent study by Stockdale et al (2007) has demonstrated that T. foetus isolated from a cat was able to cause endometritis and vaginitis upon experimental infection of heifers, although the endometrial damage caused by the cat isolate was less severe than that by T. foetus isolated from cattle in a parallel experiment. Conversely, T. foetus isolated from cattle could only successfully infect two out of five cats upon experimental infection (Stockdale et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

et al. 2008

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Tritrichomonas foetus, a parasite well known for its significance as venereally transmitted pathogen in cattle, has recently been identified as a cause of chronic largebowel diarrhea in domestic cats in the US, UK, and, more recently, also in Norway. In a period of 3 months (October to December 2007), 45 cats of Switzerland suffering from chronic diarrhea were investigated for intestinal infections, including a search for trichomonads. A commercially available in vitro culture system was used to screen for infection, complemented with a PCR and subsequent amplicon sequencing to support speciation. The PCR is based upon amplification of a sequence derived from the internal transcribed spacer region 1 (ITS1) on the ribosomal RNA gene (rRNA) using primers designed to detect a broad range of genera and species belonging to the family of Trichomonadidae. The method was furthermore adapted to the uracil DNA glycosylase (UDG) system in order to prevent carry-over contamination and it included a recombinant internal control to track for inhibitory reactions. Eleven out of the 45 cats were culture-positive, as revealed by microscopic identification of trichomonadid organisms. One of the isolates was subjected to scanning electron microscopy and findings revealed the presence of three flagella, thus placing the isolate into the gender Tritrichomonas sp. PCR and subsequent amplicon sequencing were carried out with ten of the 11 isolates. A total homology with published T. foetus sequences was confirmed in all of the cases. T. foetus therefore appears to range among those organisms that can cause chronic diarrhea in cats in Switzerland.

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Section: Discussionmentioning

confidence: 99%

Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

et al. 2008

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“…The results of the present study strongly suggest that T. suis may be fairly common in diarrheic cats in Japan. In addition, a recent study demonstrated that a T. suis isolate from a cat was capable of causing endometritis and vaginitis upon experimental infection of heifers; however, the endometrial damage caused by the cat isolate was less severe than that caused by T. suis isolated from cattle in a parallel experiment [16]. Conversely, T. suis isolated from cattle could only successfully infect two out of five cats upon experimental infection [14].…”

Section: Discussionmentioning

confidence: 99%

Intestinal <i>Tritrichomonas suis </i>(=<i>T. foetus</i>) Infection in Japanese Cats

Doi

Hirota

Morita

et al. 2012

The Journal of Veterinary Medical Science

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abstract. Tritrichomonas suis (=T. foetus) has recently been reported to be a causative agent of chronic large-bowel diarrhea in cats. While the disease was previously attributed to Pentatrichomonas hominis, the etiologic agent for feline trichomonal diarrhea was identified as T. suis. Although feline trichomonosis due to T. suis has been reported at prevalences ranging from 14 to 31% in Europe and the U.S., no reports of the pathogen have been published to date in Japan. In 2008, however, we encountered a case of feline trichomonosis at the Veterinary Teaching Hospital of Hokkaido University. The parasite was identified as T. suis by nested PCR amplification of partial internal transcribed spacer region 1 and 5.8S ribosomal RNA gene sequences with T. suis-specific primers and DNA sequencing of the amplified products. We then conducted surveys for feline trichomonosis in three different animal hospitals using either cultivation and/or PCR-based assays. The results revealed that 13 of 147 samples (8.8%) were positive for T. suis, and that 5 of the 13 infected cats, which ranged between 1 month and 7.5 years-old, showed chronic diarrhea. Seven of the infected cats were purebred and 6 were mixed breed. These findings suggested that feline trichomonosis is prevalent in Japan, and that T. suis may play a role as a causative agent of feline chronic diarrhea.

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“…Some concerns for cattle health had arisen after the detection of widespread infection of cats with feline T. foetus (Burgener et al, 2009;Frey et al, 2009), as cats on farms normally have unlimited access to barns and cattle, and as experimental transmission from cats to cattle is possible (Stockdale et al, 2007). However, recent studies unambiguously demonstrated that feline and bovine T. foetus exhibit conserved genetic differences (Reinmann et al, 2012;Slapeta et al, 2010Slapeta et al, , 2012Sun et al, 2012), thus suggesting different host tropism (Slapeta et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Tritrichomonas foetus: Prevalence study in naturally mating bulls in Switzerland

Bernasconi

Bodmer

Doherr

et al. 2014

Veterinary Parasitology

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Switzerland is officially free from bovine Tritrichomonas foetus. While bulls used for artificial insemination (AI) are routinely examined for this pathogen, bulls engaged in natural mating, as well as aborted fetuses, are only very sporadically investigated, indicating that the disease awareness for bovine tritrichomoniasis is low. Natural mating in cattle is becoming increasingly popular in Switzerland. Accordingly, a re-introduction/re-occurrence of T. foetus in cattle seems possible either via resurgence from a yet unknown bovine reservoir, or via importation of infected cattle. The low disease awareness for bovine tritrichomoniasis might favor an unnoticed re-establishment of T. foetus in the Swiss cattle population. The aim of our study was thus to search for the parasite, and if found, to assess the prevalence of bovine T. foetus in Switzerland. We included (1) bulls over two years of age used in natural mating and sent to slaughter, (2) bulls used for natural service in herds with or without fertility problems and (3) aborted fetuses. Furthermore, the routinely examined bulls used for AI (4) were included in this study. In total, 1362 preputial samples from bulls and 60 abomasal fluid samples of aborted fetuses were analyzed for the presence of T. foetus by both in vitro cultivation and molecular analyses. The parasite could not be detected in any of the samples, indicating that the maximal prevalence possibly missed was about 0.3% (95% confidence). Interestingly, in preputial samples of three bulls of category 1, apathogenic Tetratrichomonas sp. was identified, documenting a proof-of-principle for the methodology used in this study.

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Experimental Infection of Cattle With a Feline Isolate of Tritrichomonas Foetus

Cited by 46 publications

References 17 publications

Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

Intestinal <i>Tritrichomonas suis </i>(=<i>T. foetus</i>) Infection in Japanese Cats

Tritrichomonas foetus: Prevalence study in naturally mating bulls in Switzerland

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