Experimental Infection in Mice with <i>Bacillus licheniformis</i>

Agerholm, Jørgen Steen; Jensen, Henrik Elvang; Jensen, N. E.

doi:10.1111/j.1439-0450.1995.tb00708.x

Cited by 11 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pathological changes were in general similar to those previously described (1). It was obvious that hepatitis developed following immunodepression probably due to haematogenous spread of intestinal microorganisms which penetrated the intestinal barrier.…”

Section: Discussionsupporting

confidence: 84%

“…Immunostaining was performed using a peroxidase-antiperoxidase (PAP) technique, working with a primary polyclonal antibody raised in rabbits against somatic antigens of B. licheniformis (ATCC 14580) (1 ). Sections of formalin-fixed and paraffin-embedded murine brain tissue experimentally infected with B. lichenijormis (ATCC 14580) (Agerholm et a/., unpublished) were used as controls and run in parallel in all immunostainings performed.…”

Section: I~imunohistocliemistr~~mentioning

confidence: 99%

See 1 more Smart Citation

A preliminary study on the pathogenicity of Bacillus licheniformis bacteria in immunodepressed mice

Agerholm¹,

Jensen²,

Giese³

et al. 1997

APMIS

Self Cite

View full text Add to dashboard Cite

preliminary study on the pathogenicity of Bucillus licheniformis bacteria in immunodepressed mice. APMIS 105: [48][49][50][51][52][53][54] 1997.The pathogenicity of 13 strains of Bacillus licheniformis was studied in immunodepressed mice. The strains had been isolated from cases of bovine abortions (n=5), bovine feedstuffs (n=3), soil (n= l), and grain products (n=2). The origin of two strains was unknown. Groups of 10 mice were inoculated intravenously with B. licheniformis bacteria at doses from < lo6 to 10" colony-forming units. Following 7 days of infection, the animals were euthanized and examined bacteriologically, histologically, and immunohistochemically using a PAP technique based on primary polyclonal rabbit anti-B. licheniformis antibodies. B. lichen[formis bacteria were reisolated from the liver, spleen, or kidneys of mice in all groups. Inflammatory lesions were present in mice of all immunodepressed groups, but only brain and pulmonic lesions were definitely attributed to B. licheniformis infection, as strong immunostaining was found within these lesions. It is concluded that all strains of B. licheniformis examined were pathogenic for immunodepressed mice, and that spontaneous infections may be established by bacterial strains to which susceptible individuals are accidentally exposed.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: I~imunohistocliemistr~~mentioning

confidence: 99%

A preliminary study on the pathogenicity of Bacillus licheniformis bacteria in immunodepressed mice

Agerholm¹,

Jensen²,

Giese³

et al. 1997

APMIS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The higher sensitivity and specificity of unabsorbed antisera raised against somatic antigens (enzymes and intra-cellular structural components) is in accordance with previous immunohistochemical studies of bovine pathogenic bacteria, in which antisera against somatic and wall antigens of Bacillus Iicheniformis and A . pyogenes, were produced (1,14). The slight cross-reactivity with Pasteurella bacteria of the antiserum raised against the somatic antigens of H. somnus (939/90) probably resulted from the presence of small LPS-fractions within the preparation of somatic antigens used for immunization.…”

Section: Discussionmentioning

confidence: 99%

Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Tegtmeier¹,

Jensen²,

Jensen

1995

APMIS

View full text Add to dashboard Cite

A peroxidase-antiperoxidase (PAP) technique was developed for the identification of Haemophilus somnus bacteria in lung tissues of calves. Antisera raised against somatic and wall antigens of a Danish and American strain of H. somnus were produced. Experimentally infected murine tissues were used for the determination of the sensitivity and specificity of antiserum that had been heterologously absorbed with antigens of cross-reacting bacteria, i.e. Pasteurella haemolytica and Pasteurella multocida. None of the antisera reacted with Actinomyces pyogenes. An antiserum raised against somatic antigens of the Danish strain of H. somnus revealed the highest sensitivity in the PAP technique and became specific following absorption. Heterologous absorption also rendered this antiserum specific in crossed immunoelectrophoresis. Subsequently, the PAP technique was applied on formalin-fixed pneumonic lung tissues of 86 calves. An immunodiagnosis of H. somnus pneumonia was obtained in 15 of 17 lungs from which the bacterium had been isolated. Moreover, immunostained bacteria were also demonstrated in 20 lungs from which H. somnus had not been isolated. Thus, application of immunohistochemistry significantly enhanced the diagnostic sensitivity of H. somnus pneumonia of calves and should be used as a potent supplementary tool for the routine screening of suspected lung tissues of calves from which bacterial isolation is negative.

show abstract

“…However, without prior lesions it has no potential to penetrate the body 's outer barriers. However, abortions in sheep and cattle have been documented, and it has been demonstrated experimentally that Bacillus licheniformis is capable of infecting bovine placenta (Syrjälä et al, 2007;Agerholm et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance profile of local thermophilic Bacillus licheniformis isolated from Maysan province soil

2020

View full text Add to dashboard Cite

The key concern for public health is that bacterial strains isolated from various ecosystems are immune to antibiotics used in human medicine, thus dramatically limiting therapeutic options and threatening the lives of infected people. The present study aims to reveal the antibiotics profile of fiftysix isolates of local thermophilic Bacillus licheniformis isolated from different environmental soil sites in Maysan city, Iraq. The antimicrobial agent resistance profile of B. licheniformis isolates was performed using the disc diffusion assay according to Kirby-Bauer susceptibility test protocol. The results showed that isolates were resistance against cefepime (n=56; 100%), amoxicillin (n=13; 23.3%) and ampicillin (n=52; 92.9%); and intermediate (n=56; 100%) against cephalothin and naldixic acid. The percentage resistance was low for aztreonam (n=4; 7%), chloramphenicol (n=3; 5%), clotrimazole (n=6; 10%), novobiocin (n=2; 3.5%) and ticarcillin (n=3; 5%). On the other hand, all isolates were sensitive (n=56; 100%) towards the following antibiotics: amikacin, ceftazidime, ciprofloxacin, clindamycin, imipenem, netilmycin, gentamicin, nitrofurantion, rifampin, trimethoprim and vancomycin. The results of this study suggest that the Iraqi thermophilic B. licheniformis isolates are variable in their susceptibility towards the standards antimicrobial agents. Furthermore, the presence of cefepime, amoxicillin, ampicillin, cephalothin and naldixic resistant isolates of B. licheniformis in Iraqi soils is of concern about how resistance could spread to other bacteria, and ultimately to humans.

show abstract

Experimental Infection in Mice with Bacillus licheniformis

Cited by 11 publications

References 18 publications

A preliminary study on the pathogenicity of Bacillus licheniformis bacteria in immunodepressed mice

A preliminary study on the pathogenicity of Bacillus licheniformis bacteria in immunodepressed mice

Development of a peroxidase‐antiperoxidase (PAP) technique for the identification of Haemophilus somnus in pneumonic calf lungs in Denmark

Antibiotic resistance profile of local thermophilic Bacillus licheniformis isolated from Maysan province soil

Contact Info

Product

Resources

About