Experimental infection in Cavia porcellus by infected Amblyomma ovale nymphs with Rickettsia sp. (Atlantic rainforest strain)

Brustolin, Joice Magali; Krawczak, Felipe da Silva; Alves, Marta Elena Machado; Weiller, M; Souza, Camila Lopes de; Rosa, Fernanda A.; Cadore, Gustavo Cauduro; Lopes, Sônia Terezinha dos Anjos; Labruna, Marcelo Bahia; Vogel, Fernanda Silveira Flores; Botton, Sônia de Ávila; Sangioni, Luís Antônio

doi:10.1007/s00436-017-5741-2

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…One year later R. parkeri was isolated from Amblyomma ovale ticks collected on a free-roaming domestic dogs in a rural area from Colombia (Londoño et al, 2014). Experimental studies have shown that A. ovale is a reservoir and competent vector of R. parkeri strain Atlantic rainforest (Krawczak et al, 2016;Brustolin et al, 2018). Endemic regions of R. parkeri strain Atlantic rainforest are associated with rural areas, especially areas with vegetation (forest, pastures) where the adult stage of A. ovale attaches to and feeds on dogs, which typically become infested in the forest (Szabó et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

Arroyave

Cornwell

McBride

et al. 2020

Rev. Bras. Parasitol. Vet.

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Tick-borne rickettsial pathogens (TBRP) are important causes of infections in both dogs and humans. Dogs play an important role as a biological host for several tick species and can serve as sentinels for rickettsial infections. Our aim was to determine the presence of TBRP in dogs and in dog-associated ticks and their potential risk to human diseases in Medellin, Colombia. DNA for E. canis (16S rRNA and dsb) and A. platys (groEl) was detected in 17.6% (53/300) and 2.6% (8/300) of dogs, respectively. Antibodies against Ehrlichia spp. 82 (27.3%) and Anaplasma spp. 8 (2.6%) were detected in dogs. Antibody reactivity against both agents were found in 16 dogs (5.3%). Eight dogs showed antibody for Rickettsia spp. with titers that suggest 3 of them had a probable exposure to R. parkeri. Rhipicephalus sanguineus s.l. (178/193) was the main tick in dogs, followed by R. microplus (15/193). The minimum infection rates (MIR) in R. sanguineus were 11.8% for E. canis and 3.4% for A. platys. E. canis and A. platys are the main TBRP infecting dogs and ticks and R. sanguineus s.l. is likely involved in the transmission of both agents. Interestingly, we found serological evidence of exposure in dogs for spotted fever group rickettsiae.

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Section: Discussionmentioning

confidence: 99%

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

Arroyave

Cornwell

McBride

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…Furthermore, subclinical infection was observed in an additional study that utilized the natural mode of infection of guinea pigs by tick transmission, using A . ovale nymphs infected with the Brazilian Atlantic Rainforest strain [20].…”

Section: Discussionmentioning

confidence: 99%

“…Previous animal model publications, where R . parkeri was used as infectious agent, did not state the biosafety level used [19,20], but it is well accepted that the researchers use this agent at biosafety level 2 containment [21]. The aim of this study was to characterize the pathogenicity of RpARF and describe the clinical course, outcome, pathologic lesions, and anatomic distribution of rickettsiae in this new mouse model of SFG rickettsiosis that can be performed at biosafety level 2.…”

Section: Introductionmentioning

confidence: 99%

A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest strain

et al. 2019

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Background The species of the Rickettsia genus are separated into four groups: the ancestral group, typhus group, transitional group and spotted fever group. Rickettsia parkeri , a spotted fever group Rickettsia , has been reported across the American continents as infecting several tick species and is associated with a relatively mild human disease characterized by eschar formation at the tick feeding site, regional lymphadenopathy, fever, myalgia and rash. Currently, there are several mouse models that provide good approaches to study the acute lethal disease caused by Rickettsia , but these models can only be performed in an animal biosafety level 3 laboratory. We present an alternative mouse model for acute lethal rickettsial disease, using R . parkeri Atlantic Rainforest strain and C3H/HeN mice, with the advantage that this model can be studied in an animal biosafety level 2 laboratory. Principal findings In the C3H/HeN mouse model, we determined that infection with 1x10 6 and 1x10 7 viable R . parkeri Atlantic Rainforest strain organisms produced dose-dependent severity, whereas infection with 1x10 8 viable bacteria resulted in a lethal illness. The animals became moribund on day five or six post-infection. The lethal disease was characterized by ruffled fur, erythema, labored breathing, decreased activity, and hunched posture, which began on day three post-infection (p.i.) and coincided with the peak bacterial loads. Significant splenomegaly (on days three and five p.i.), neutrophilia (on days three and five p.i.), and thrombocytopenia (on days one, three and five p.i.) were observed. Significance Since R . parkeri is used at biosafety level 2, the greatest advantage of this inbred mouse model is the ability to investigate immunity and pathogenesis of rickettsiosis with all the tools available at biosafety level 2.

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“…This strain has a close genetic identity with the Brazilian Atlantic rainforest strain [4] and the Colombian RpARFL isolate [6]. Furthermore, subclinical infection was observed in an additional study that utilized the natural mode of infection of guinea pigs by tick transmission, using A. ovale nymphs infected with the Brazilian Atlantic rainforest strain [17].…”

Section: Discussionmentioning

confidence: 99%

A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest-like isolate

Londoño

Mendell

Walker

et al. 2018

Preprint

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12 13 Background 14 15The species of the Rickettsia genus is separated into four groups: the ancestral group, 16 typhus group, transitional group and spotted fever group. Rickettsia parkeri, a spotted 17 fever group Rickettsia, has been reported across the American continents as infecting 18 several tick species and is associated with a relatively mild human disease characterized 19 by eschar formation at the tick feeding site, fever, myalgia and rash. Currently several 20 mouse models that provide a good approach to study the acute lethal disease caused by 21 Rickettsia, but these models can only be performed in an animal biosafety level 3 22 laboratory. We present an alternative mouse model for acute lethal rickettsial disease, 2 23 using R. parkeri and C3H/HeN mice, with the advantage that this model can be studied 24 in an animal biosafety level 2 laboratory. 25 26 Principal findings 27 28 In the C3H/HeN mouse model, we determined that infection with 1x10 6 and 1x10 7 viable 29 R. parkeri Atlantic Rainforest-like isolate produced dose-dependent severity, whereas 30 infection with 1x10 8 viable bacteria resulted in a lethal illness. The animals became 31 moribund on day five or six post-infection. The lethal disease was characterized by ruffled 32 fur, erythema, labored breathing, decreased activity, and hunched back, which began on 33 day three post-infection (p.i.) and coincided with the peak bacterial loads. Significant 34 splenomegaly (on days three and five p.i.), neutrophilia (on days three and five p.i.), and 35 thrombocytopenia (on days one, three and five p.i.) were observed. 36 37 Significance 38 39The greatest advantage of this inbred mouse model is the ability to investigate immunity 40 and pathogenesis of rickettsiosis with all the tools available at biosafety level 2. 41 42 Author summary 43 44Rickettsia is a bacterial genus that contains distinct species that are transmitted by 45 arthropods. Many of these agents produce infection and disease in humans. The illness 3 46 can range from very aggressive, such as Rocky Mountain spotted fever caused by 47 Rickettsia rickettsii, to mild human disease characterized by eschar formation at the tick 48 feeding site and less severe symptoms caused by Rickettsia parkeri. To study these 49 diseases, animal models are invaluable, and mouse models offer the best advantages. 50Several mouse models are most useful for study of the acute lethal disease produced by 51 these bacteria, providing the opportunity to test different treatments and vaccine 52 candidates. However, work with these models requires an animal biosafety level 3 53 laboratory. In this report, we present an alternative mouse model to study acute lethal 54 spotted fever group rickettsial disease with the advantage that experiments can be 55 performed at biosafety level 2. 56 57 63 64 Rickettsia parkeri, a member of the SFG, has been reported across the American 65 continents as infecting several tick species including the Amblyomma maculatum 66 complex (A. maculatum, A. triste, A. tig...

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Experimental infection in Cavia porcellus by infected Amblyomma ovale nymphs with Rickettsia sp. (Atlantic rainforest strain)

Cited by 6 publications

References 29 publications

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest strain

A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest-like isolate

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