Experimental induction of arthritis in LEW rats and antibody response to four Mycoplasma arthritidis strains

Washburn, Leigh Rice; Ramsay, James R.

doi:10.1016/0378-1135(89)90017-5

Cited by 13 publications

(22 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mycoplasmas are wall-less bacteria which are mostly harmless commensals but can also be associated with clinical symptoms from nongonococcal urethritis (12) to rheumatoid arthritis. One species, Mycoplasma arthritidis, has been studied particularly well with respect to its arthritogenic properties (47). It releases a superantigen whose structure has been elucidated and whose functional domains were identified (4).…”

mentioning

confidence: 99%

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

et al. 1999

View full text Add to dashboard Cite

Mycoplasmas may be associated with rheumatoid arthritis in various animal hosts. In humans, mycoplasma arthritis has been recorded in association with hypogammaglobulinemia. Mycoplasma fermentans is one mycoplasma species considered to be involved in causing arthritis. To clarify which mycoplasmal compounds contribute to the inflammatory, bone-destructive processes in arthritis, we used a well-defined lipopeptide, 2-kDa macrophage-activating lipopeptide (MALP-2) from M. fermentans, as an example of a class of macrophageactivating compounds ubiquitous in mycoplasmas, to study its effects on bone resorption. MALP-2 stimulated osteoclast-mediated bone resorption in murine calvaria cultures, with a maximal effect at around 2 nM. Antiinflammatory drugs inhibited MALP-2-mediated bone resorption by about 30%. This finding suggests that MALP-2 stimulates bone resorption partially by stimulating the formation of prostaglandins. Since interleukin-6 (IL-6) stimulates bone resorption, we investigated IL-6 production in cultured calvaria. MALP-2 stimulated the liberation of IL-6, while no tumor necrosis factor was detectable. Additionally, MALP-2 stimulated low levels of NO in calvaria cultures, an effect which was strongly increased in the presence of gamma interferon, causing an inhibition of bone resorption. MALP-2 stimulated the bone-resorbing activity of osteoclasts isolated from long bones of newborn rats and cultured on dentine slices without affecting their number. In bone marrow cultures, MALP-2 inhibited the formation of osteoclasts. It appears that MALP-2 has two opposing effects: it increases the bone resorption in bone tissue by stimulation of mature osteoclasts but inhibits the formation of new ones.

show abstract

mentioning

confidence: 99%

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

et al. 1999

View full text Add to dashboard Cite

show abstract

“…It is a naturally occurring arthritogen of rats and mice (Cole & Ward, 1973) that has previously been demonstrated to show antigenic differences among strains (Washburn & Hirsch, 1990 ;Stadtlander & Watson, 1992 ;Washburn e t al., 1995). Experimentally-induced M. artbritidis arthritis exemplifies the prominent features associated with many mycoplasma infections (Cole e t al., 1985a, b) and is therefore widely used as model for studying pathogenic mechanisms (Kirchhoff et al, 1983a(Kirchhoff et al, , b, 1984(Kirchhoff et al, , 1989Washburn & Ramsey, 1989;Washburn e t al., 1993;Cole & Griffiths, 1993). Since this model is well characterized and easy to manipulate, its use should help increase understanding of the biological functions of mycoplasma antigenic variation and its possible relationship to natural disease.…”

Section: Introductionmentioning

confidence: 99%

“…These diseases typically Several species of mycoplasmas are well-established involve arthritis, respiratory or genitourinary infections, pathogens, causing diseases in man and other animals and often show immunopathological features and On: Sun, 13 May 2018 07:46:02 M. DROESSE a n d OTHERS sequelae characteristic of chronic disease (Cole e t al., 1985a, b). Although several reports have indicated the immune response, nonspecific of T and B lymphocytes and induction of autoantibodies as factors contributing to the disease process (Cole e t al., 1985a,..b;.----substrains D263, ISR1, ISRlpl, ISRlp7, ISRlp8 and 1 5 8~1 0 are highly arthritogenic for rats, strains PG6 and I-KO6 are 1983a;Washburn & Ramsey, 1989;Binder etal., 1990b;Droesse e t al., 1994). The virulence of substrain D263pl has not been examined.…”

Section: Introductionmentioning

confidence: 99%

Major membrane proteins and lipoproteins as highly variable immunogenic surface components and strain-specific antigenic markers of Mycoplasma arthritidis

et al. 1995

View full text Add to dashboard Cite

Ha n nover, B isc hof s hole r Damm 15,30173 Hannover, Germany Microbiology, Surface antigenic variation was investigated in Mycop/asma arthritidis, an agent that produces chronic arthritis in rats which shares several features with many mycoplasma-induced diseases and thus defines a well-characterized model system. Hyperimmune rabbit antisera (anti-ISRI, anti-PG6, anti-H606 and anti-158~10) to whole M. arthritidis organisms were used as immunological probes in Western immunoblots of four M. arthritidis prototype strains (ISRI, PG6, H606 and D263) and five rat-passaged substrains (ISRlpl, ISRlp7, ISRlp8, 1 5 8 ~ 1 0 and D263pl). Several prominent antigens were identified that varied in expression. By Triton X-114 phase fractionation and treatment of whole cells with trypsin and carboxypeptidase Y, these strain-variant antigens were shown to be integral membrane proteins with C-termini and portions of the polypeptide chains oriented outside the membrane. Western blot immunoscreening of a large number of randomly selected clonal isolates and well-established clonal lineages from stock cultures of M. arthritidis ISRlp7, 158~10, PG6 and H606 revealed an expanded repertoire of variant membrane proteins whose expression was subject to independent, reversible phase variation. Colony immunoblots of these clonal populations with a hyperimmune rabbit antiserum to a gel-purif ied variant membrane protein (P36) showed that this phase switching occurred at a high frequency lo-* per generation). Detailed immunological and biochemical characterization of the phase-variant membrane proteins demonstrated that they are: (i) antigenically related or distinct; (ii) apparently specific to particular strain populations; (iii) proteins or lipoproteins; (iv) major immunogens of M. arthritidis, recognized by serum antibodies from convalescent rat; and (v) able to undergo variation in expression during in viwo passage. Thus, M. arthritidis possesses a complex system capable of creating large repertoires of cell surface phenotypes which may affect the multiple interactions of this organism with its host and dictate its potential as a successful infectious agent and pathogen. to

show abstract

“…In addition, two potentially relevant cases positive for antibody to HIV have been described, one being a 27 year old homosexual man with diarrhoea, urethritis, conjunctivitis, arthritis, and cryptosporidial enteritis,2 and the other a 4 year old child (sex unknown) with cryptosporidiosis who developed conjunctivitis as part of a more profound systemic illness (which was subsequently diagnosed as measles). 3 Our patient had conjunctivitis and scattered arthralgic and myalgic pains associated with her gastroenteritic illness; accordingly, although the pathogenesis of cryptosporidial enteritis in immunocompetent subjects remains poorly understood, it is feasible that Reiter's syndrome may represent a potential clinical consequence of the condition. It may be pertinent to note that unlike the almost total male exclusivity of Reiter's syndrome associated with urethritis, among cases of Reiter's syndrome associated with gastroenteritis (of whatever underlying cause) a much larger proportion occur in women.4 In addition, the syndrome has been reported occurring in sexually inactive children.4 STEPHEN Raised plasma concentrations of endothelin-1 in systemic lupus erythematosus Sir: Endothelin-I is a newly described vasoconstrictor peptide produced and secreted by vascular endothelial cells.'…”

mentioning

confidence: 89%

Raised plasma concentrations of endothelin-1 in systemic lupus erythematosus.

Julkunen¹,

Saijonmaa²,

Grönhagen-Riska³

et al. 1991

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

Experimental induction of arthritis in LEW rats and antibody response to four Mycoplasma arthritidis strains

Cited by 13 publications

References 21 publications

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

Effect of MALP-2, a Lipopeptide from Mycoplasma fermentans , on Bone Resorption In Vitro

Major membrane proteins and lipoproteins as highly variable immunogenic surface components and strain-specific antigenic markers of Mycoplasma arthritidis

Raised plasma concentrations of endothelin-1 in systemic lupus erythematosus.

Contact Info

Product

Resources

About