Experimental In vitro Study to Assess the Antibacte-rial Activity of Thymus vulgaris Oil on Streptococ-cus Sanguinis

Abstract

Background: The Streptococcus genus are the predominant bacteria in the mouth and the Streptococcus sanguinis is one of them which performing a primary function for expansion of dental biofilm. Gingival and periodontal disorders are caused by dental biofilm, today, there is a necessity to discover naturally presenting antibacterial compounds from herbs with less side effects as a substitutive to the commonly handled chlorohexidine. Thus, the purpose of this study was to assess the antibacterial activity of thymus vulgaris oil on Streptococcus sanguinis bacteria In vitro. Materials and Methods: Human supragingival plaque samples were taken from 10 subjects, then morphological and microscopical examination, biochemical tests, Optochin test, hemolytic ability test and conventional polymerase chain reaction test was applied to assure coincidence of Streptococcus sanguinis. The sensitivity of bacteria to Thymus vulgaris oil, the minimum concentration that inhibit the growth and killed the test bacteria were identified a partly as comparable to 0.2% chlorhexidine as a positive control and10 % dimethyl sulphoxide as a negative control. Results: Thymus vulgaris oil presented a significant antibacterial property on Streptococcus sanguinis with several grades of inhibition zones. When compared to Chlorohexidine, Thymus vulgaris oil had stronger antibacterial properties. The minimum concentration that inhibited the growth and killed Streptococcus sanguinis was (0.09%). Conclusion: Thymus vulgaris oil displayed higher antibacterial action with each concentration on Streptococcus sanguinis as compared to Chlorohexidine, therefore; it can be used as a natural substitutive oral health care product to Chlorohexidine.

Comparison of Antifungal Activity of Thymus Vulgaris Essential Oil and Triple Antibiotic Paste Against Candida Albicans Isolated from Root Canal (In Vitro Study)

“…22 Our outcome is consistent with a previous study that found a direct relationship between concentration and antimicrobial activity. 19 Mith et al (2014) used the agar diffusion method to examine the antimicrobial effects of TVEO on several microorganisms and found that TVEO has antimicrobial properties that result in greater suppression of bacterial growth as TVEO concentration increases. 23 This result shows considerable promise for using TVEO as a replacement for TAP.…”

“…After culturing, the concentration was considered to be MFC if it was equal to or higher than the MIC and there was no fungal growth on SDA. 19 Statistical analysis SPSS (Statistical Package for social Science) (version -22, Chicago, Illionis, USA) was used. Minimum, maximum, standard error, mean, and standard deviation, as well as the Levene test, Shapiro-Wilk test, and Tukey HSD with one-way analysis of variance (ANOVA) were used for data description, analysis, and presentation.…”

Synergistic Antimicrobial and Antibiofilm Activity of Panax Ginseng, Symphytum Officinale, and Metronidazole against P. gingivalis

“…They demonstrated a synergistic interaction with the antibiotic Metronidazole, greatly boosting its antibacterial effectiveness [2]. This information may be significant because using plant extracts [28] in conjunction with antibiotics may help to combat the rising issue of antibiotic resistance [3].…”