Experimental immunology Xenotransplantation of human cultured parathyroid progenitor cells into mouse peritoneum does not induce rejection reaction

Abstract

IntroductionParathyroid progenitor cells devoid of immunogenic antigens were used for human allotransplantation. Although there were many potential reasons for the expiry of transplant activity in humans, we decided to exclude a subclinical form of rejection reaction, and test the rejection reaction in an animal model.Material and methodsExperiments were carried out on 40 conventional male mice in their third month of life. The animals were housed in groups of 10 per cage in 4 cages with fitted water dispensers and fed a conventional diet based on standard pellet food. They were divided into four groups of 10 animals each, three experimental groups and one control group. Identified progenitor cells were stored in a cell bank. After testing the phenotype, viability, and absence of immunogenic properties, the cells were transplanted into mouse peritoneum cavity.ResultsAnimals were observed for 9 weeks. At 9 weeks of observation, the mean serum PTH concentration in the experimental groups was 2.0-2.5 pg/ml, while in the control group it did not exceed 1.5 pg/ml. The immunohistochemical assays demonstrated that millions of viable cells with a phenotype identical to the endocrine cells had survived in the peritoneum. Histologic specimens from different internal organs stained for PTH revealed positive cells labelled with anti-PTH Ab in the intestinal lamina, brain, liver, and spleen.ConclusionsIn the present paper we have demonstrated that xenotransplantation may be used as a model for an explanation of the immunogenic properties of cells generated from postnatal organs for regenerative therapy.

Microencapsulated parathyroid allotransplantation in the omental tissue

“…Furthermore, transplanted cells are not exposed to high pressure and/or mechanical trauma. One report demonstrated that mouse peritoneum does not induce a rejection reaction to PA and murine abdominal cavities may act as a natural incubator for transplanted cells and in our case for microencapsulated parathyroid cells as well.…”

Introductory Chapter: A Brief Statement about Parathyroid Glands

“…In 2017, they reported the survival results of 316 allotransplantation data. Since the early 1990s, many research projects have been added to the literature about cell isolation, graft delivery location, and follow-up parameters from the same group [29][30][31][32][33]. During the last five years of the parathyroid transplantation, advanced immunological transplantation criteria, including pre-op and post-follow-up processes after parathyroid transplantation, were brought to the literature by the same group from Bezmialem Vakif University in Turkiye [22,[34][35][36][37][38][39][40][41][42][43] Parathyroid transplantation is the most effective and targeted hypoparathyroidism treatment in the literature due to limited access to hormone replacement therapy and ongoing phase studies.…”

Comparison Between Two Different Second-Generation Parathyroid Hormone Assays

“…측정가능범위는 0.7-1,500 pg/mL이며, 참고치는 9-55 pg/mL 이다. ELSA-PTH는 (1-84) PTH와 (7-84) PTH를 같이 검출하며, (1-34), (39-68), (53-84), (44-68), (39-84) PTH 형태의 조각들과는 교 차반응을 일으키지 않는 것으로 보고되었다 [11].…”