Experimental immunology Acute meningitis in rats is associated with decreased levels of miR132 and miR146a

Abstract

IntroductionThe pathogenesis of bacterial meningitis due to Streptococcus pneumoniae is still unclear. Despite early treatment with antibiotics, its morbidity and mortality is still high.Material and methodsStreptococcus pneumoniae induced rat meningitis models were taken and divided into 2 groups; control (C) and meningitis (M). Western blot was used to detect toll like receptor 4 (TLR4), tumor necrosis factor α (TNF-α), interleukin 1B (IL1B), nuclear factor κB (NFκB) and real time polymerase chain reaction were used to detect the expression of miR146a, miR132, respectively.ResultsWe found that the expressions of TLR4, TNF-α, IL1B, NFκB were all up-regulated in the acute stage of bacterial meningitis when compared to the control group. While for the post transcription factors, miR146a and miR132, the opposite was observed. They were down-regulated in the meningitis group.ConclusionsmiR146a and miR132 may take part in the pathogenesis of SP bacterial meningitis as well as the TLR4- NFκB- TNF-α/IL1B signal transduction pathway.

Respiratory syncytial virus infection‐induced mucus secretion by down‐regulation of miR‐34b/c‐5p expression in airway epithelial cells

“…Total miRNAs was prepared with TRIzol reagent (Thermo Fisher Scientific, USA) and Phenol-Chloroform extraction from HBECs and quantified on a SmartSpec™ Plus spectrophotometer (Bio-rad, USA). 25 cDNA was reverse transcribed by use of the miRcute miRNA cDNA kit (Tiangen Biotech, China), and RT products were then detected by the miRcute Plus miRNA qPCR Kit (Tiangen Biotech, China). U6 was used as an internal reference.…”

Epigenetic Alterations in Juvenile Spondyloarthritis Patients: a Preliminary Study of Selected Genes Promoter Methylation and Silencing

“…The four microRNAs tested in the present study (miR-150, miR-146a, miR-181a, and miR-223) were prudently selected based on previously reported interactions with NLRP3, TLR4, PTPN12, or CXCR4 gene [28][29][30][31][32][33][34]. However, the lack of the expression alterations does not exclude the possible role of this or other microRNAs in post-transcriptional regulation of the selected genes but warrants additional studies.…”