Experimental immunisation of lambs against pneumonic pasteurellosis

Gilmour, N.J.L.; Martin, William B.; Sharp, J. M.; Thompson, D.A.; Wells, P.W.; Donachie, W.

doi:10.1016/s0034-5288(18)32208-2

Cited by 48 publications

(14 citation statements)

References 8 publications

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“…Suboptimal efficacy of some pasteurella vaccines against experimental A2 challenge [4] may be explained by the limited range of antigens present on the surface of bacterial cells grown in iron replete media. In contrast, the increased number and strong immunogenicity of antigens seen on the surface of 'in vivo' grown cells may be essential for an effective vaccine against pasteurellosis.…”

Section: Discussionmentioning

confidence: 99%

“…Pleural fluid was aspirated from caesarean-derived colostrum-deprived, specific pathogen-free (SPF) lambs with experimental pneumonic pasteurellosis [4]. Up to 400 ml of the fluid was collected in a glass bottle containing 2 ml of 100 ~tM-EDTA to prevent the formation of fibrin clots.…”

Section: Collection Of P Haemolytica From Pleural Fluidmentioning

confidence: 99%

“…Vaccination is the accepted method of control for pasteurellosis and homologous cell extract vaccines have protected sheep against disease caused by A biotype serotypes 1, 6 and 9 [4]. However vaccines containing antigens of serotype A2, which is responsible for the majority of outbreaks [5] have been comparatively poor immunogens in mice, rabbits and sheep [4,6,7]. A high degree of immunity against A2 serotype disease has been reported only for Iambs recovered from experimentally induced pasteurellosis [7].…”

Section: Introductionmentioning

confidence: 99%

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Sheep antibody response to cell wall antigens expressed in vivo by Pasteurella haemolytica serotype A2

Donachie¹

1988

FEMS Microbiology Letters

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Cells of Pasteurella haemolytica A2 were harvested directly from the pleural fluid of sheep with pneumonic pasteurellosis and the protein composition of their envelopes was examined by SDS‐PAGE. Several high molecular‐weight proteins expressed in pleural fluid were absent when the same isolate was grown in nutrient broth, but were produced during culture in iron restricted media. When the cell wall envelopes were examined by immunoblotting with sera and lung washes of lambs recovering from pneumonic pasteurellosis, antibodies to the major outer membrane proteins, the iron‐regulated proteins and one other ‘in vivo’‐expressed antigen were present. These results have implications for the formulation of effective vaccines against pasteurellosis in sheep.

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Section: Discussionmentioning

confidence: 99%

Section: Collection Of P Haemolytica From Pleural Fluidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sheep antibody response to cell wall antigens expressed in vivo by Pasteurella haemolytica serotype A2

Donachie¹

1988

FEMS Microbiology Letters

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“…Presence of pleurisy was scored between 0 and 3; 0 = no pleurisy and 3 being most severe. The gross lesions along the respiratory tract were described following visual examination and palpation before they were scored 16,17 . Briefly, no significant lesions = 0, 5–10% lung surface affected considered mild and scored 1 point, 11–25% considered moderate and scored 2 points and over 25% considered severe and scored 3 points.…”

Section: Methodsmentioning

confidence: 99%

“…The gross lesions along the respiratory tract were described following visual examination and palpation before they were scored. 16,17 Briefly, no significant lesions = 0, 5-10% lung surface affected considered mild and scored 1 point, 11-25% considered moderate and scored 2 points and over 25% considered severe and scored 3 points. The gastrointestinal tract was examined in a similar manner from the esophagus to the rectum and scored accordingly (0 represents no significant lesion and 3 represents most severe gross lesion).…”

Section: Post-mortem Examinationsmentioning

confidence: 99%

Clinico-pathological changes in buffalo calves following oral exposure to Pasteurella multocida B:2

Abubakar

Zamri‐Saad

2011

Basic and Applied Pathology

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Background and aim: Pasteurella multocida B:2, which causes hemorrhagic septicemia of ruminants, is believed to enter the host via respiratory and oral routes. While the role of respiratory route of infection has been established, the present study describes the clinical and pathological alterations following oral exposure of buffalo calves to live wild‐type Pasteurella multocida B:2. Methods: Nine 8‐month‐old buffalo calves were selected and divided into three groups. Calves of group 1 were exposed orally to 50 mL of the inoculums containing 109 colony‐forming unit (CFU)/mL of live Pasteurella multocida B:2. Calves of group 2 were exposed intra‐tracheal to 5 mL of the same inoculums while calves of group 3 were exposed orally to 50 mL of phosphate‐buffered saline. Results: Only one calf had to be euthanized at 48 h post‐intra‐tracheal exposure due to persistent clinical signs of hemorrhagic septicemia. The mean respiratory rate and rectal temperature had significantly increased in calves of groups 1 and 2. The pathological alterations included submandibular and brisket edema, generalized lymphadenopathy, acute fibrinous pneumonia, acute colitis and hemorrhagic typhilitis and proctitis. Nevertheless, oral infection with 109 CFU/mL of live wild‐type P. multocida B:2 failed to produce a typical clinical disease. However, P. multocida B:2 was present along the gastro‐intestinal tract, including the rectum of the calf that succumbed to the infection at 48 h post‐intra‐tracheal exposure. Conclusions: The presence of P. multocida B:2 along the gastro‐intestinal tract is of concern since transmission via the gastro‐intestinal tract of diseased animals is strongly possible.

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Commercial Development of Haemophilus, Actinobacillus and Pasteurella Vaccines

Houghton¹

1995

Haemophilus, Actinobacillus, and Pasteurella

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Experimental immunisation of lambs against pneumonic pasteurellosis

Cited by 48 publications

References 8 publications

Sheep antibody response to cell wall antigens expressed in vivo by Pasteurella haemolytica serotype A2

Sheep antibody response to cell wall antigens expressed in vivo by Pasteurella haemolytica serotype A2

Clinico-pathological changes in buffalo calves following oral exposure to Pasteurella multocida B:2

Commercial Development of Haemophilus, Actinobacillus and Pasteurella Vaccines

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