Experimental hybridization of Angiostrongylus mackerrasae, Bhaibulaya, 1968 and Angiostrongylus cantonensis (Chen, 1935)

Bhaibulaya, Manoon

doi:10.1016/0020-7519(74)90020-4

Cited by 18 publications

(29 citation statements)

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“…In some other interesting life‐history studies, cross‐breeding experiments by Bhaibulaya (1974) and Cross and Bhaibulaya (1974) have shown that viable F1 hybrids could be generated between A. cantonensis × A. mackerrasae and A. cantonensis × A. malaysiensis , respectively. However, while the F1 females appeared to produce eggs (which we take to mean oocytes), the F1 males did not produce spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Dusitsittipon

Criscione

Morand

et al. 2018

Evolutionary Applications

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Angiostrongylus cantonensis, the rat lungworm, is a zoonotic pathogen that is one of the leading causes of eosinophilic meningitis worldwide. This parasite is regarded as an emerging pathogen with a global range expansion out of southeastern Asia post‐WWII. To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, cytochrome c oxidase 1 (CO1) and cytochrome b (CYTB), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants. In order to look for more global evolutionary patterns, we herein provide a collective phylogenetic assessment using the six available whole mtDNA genome samples that have been tagged as A. cantonensis, A. malaysiensis, or A. mackerrasae along with all other GenBank CO1 and CYTB partial sequences that carry these species identifiers. The results reveal three important complications that researchers will need to be aware of, or will need to resolve, prior to conducting future molecular evolutionary studies on A. cantonensis. These three problems are (i) incongruence between taxonomic identifications and mtDNA variants (haplotypes or whole mtDNA genome samples), (ii) the presence of a CYTB mtDNA pseudogene, and (iii) the need to verify A. mackerrasae as a species along with other possible cryptic lineages, of which there is suggestive evidence (i.e., A. cantonensis could be a species complex). We provided a discussion of how these complications are hurdles to our understanding of the global epidemiology of angiostrongyliasis. We call for future studies to be more explicit in morphological traits used for identifications (e.g., provide measurements). Moreover, it will be necessary to repeat prior morphological and life‐history studies while simultaneously using sequence data in order to assess possible associations between critical epidemiological data (e.g., biogeography, virulence/pathology, host species use) and specific lineages.

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Section: Discussionmentioning

confidence: 99%

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Dusitsittipon

Criscione

Morand

et al. 2018

Evolutionary Applications

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“…Adults of only two nematode species, Angiostrongylus cantonensis and A. mackerrasae, have been reported from the lungs of rodents in Australia (Mackerras & Sandars, 1955;Bhaibulaya, 1968Bhaibulaya, , 1974Bhaibulaya, , 1975Mesina et al, 1974) although a diverse array of nematodes occur in the lungs of marsupials (Spratt, 1979(Spratt, , 1980(Spratt, , 1981(Spratt, , 1984Spratt & Gill, 1998), some causing mild pathological changes (McColl & Spratt, 1982;Spratt & Gill, 1998). The nematodes found in the pulmonary nodules of indigenous rats in New South Wales were morphologically distinct from metastrongyloid, thelazioid and muspiceoid lungworms previously recorded from rodents and marsupials in Australia (loc.…”

Section: Introductionmentioning

confidence: 99%

Gallegostrongylus australisn. sp. (Nematoda: Angiostrongylidae) from Muridae in Australia, with zoogeographical considerations

2001

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“…Forty years ago, Bhaibulaya 38 showed that A. mackerrasae was neurotropic and migrated to the brain of rats in the same manner as A. cantonensis 38 . Given the similarity in larval migratory pathways within the host of these two species, it is plausible that A. mackerrasae may also be neurotropic in humans as well as other animal hosts.…”

Section: Angiostrongylus Species In Australiamentioning

confidence: 99%

“…Since the primary points of morphological distinction between the two species are in the copulatory apparatus, it might be expected that there exists mechanical isolation between the two species. Species interbreeding has been achieved, however, when experimental hybridization of the two species produced male F1 hybrids that were sterile whereas females remained fertile 38 . Bhaibulaya 38 also observed that the morphology of F1…”

Section: Angiostrongylus Species In Australiamentioning

confidence: 99%

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Molecular and pathological studies on Angiostrongylus species in southeast Queensland

Aghazadeh¹

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Despite an apparent increase in cases of angiostrongyliasis in humans and animals in Australia, the epidemiology of infection with the two species of Angiostrongylus that co-exist in this country, namely A. cantonensis and A. mackerrasae, is poorly understood. This knowledge gap is particularly important with respect to Angiostrongylus mackerrasae, a species evidently native to Australia, as its ability to cause disease in humans is unknown.Likewise, there is little information on the roles of native and introduced species of rodents and molluscs as hosts of Angiostrongylus species in Australia. This thesis focuses on the gaps in the knowledge about the epidemiology of two species in southeast Queensland, highlighting the hotspots for transmission of the parasite in urban Brisbane. A comprehensive survey of rats belonging to the species Rattus rattus, Rattus norvegicus and Rattus fuscipes was conducted. The prevalence of infection with Angiostrongylus spp. was 16.5% in Rattus spp. trapped in urban Brisbane and surrounds which is much higher than estimates of earlier studies. This highlights the possible risk of zoonotic infection in children, dogs and wildlife in this region and indicates the necessity for public awareness as well as more detailed epidemiological studies on this parasite in eastern Australia.Of the two Australian species, Angiostrongylus mackerrasae appears to be adapted to life in the native bush rat, Rattus fuscipes. This species evidently has an identical life cycle to that of Angiostrongylus cantonensis. A. mackerrasae may have greater potential to infect non-rodent hosts as it has been found as adult form in the lungs, as has been recorded for an individual flying fox.This raises concerns as to the potential of this native angiostrongylid to infect domestic animals and humans. To date, data on the taxonomy, epidemiology and population genetics of A. mackerrasae are poor or unknown. Here, we describe the mitochondrial (mt) genome of A. mackerrasae with the aim of addressing some of these knowledge gaps.The complete mitochondrial (mt) genome of A. mackerrasae was amplified from a single morphologically identified adult worm, by long-PCR in two overlapping amplicons (8 kb and 10 kb). The amplicons were sequenced using the MiSeq Illumina platform and annotated using an in-house pipeline. Amino acid sequences inferred from individual protein coding genes of the mt genomes were concatenated and then subjected to phylogenetic analysis using Bayesian inference.The mt genome of A. mackerrasae is 13,640 bp in size and contains 12 protein coding genes (cox1-3, nad1-6, nad4L, atp6 and cob), and two ribosomal RNA (rRNA) and 22 transfer RNA (tRNA) genes. The mt genome of A. mackerrasae has similar characteristics to those of other Angiostrongylus species which is explained in detail in chapter 3. Phylogenic comparisons reveal III that A. mackerrasae is closely related to A. cantonensis and the two sibling species may have recently diverged compared with all other species in the genus with a highl...

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Experimental hybridization of Angiostrongylus mackerrasae, Bhaibulaya, 1968 and Angiostrongylus cantonensis (Chen, 1935)

Cited by 18 publications

References 3 publications

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Gallegostrongylus australisn. sp. (Nematoda: Angiostrongylidae) from Muridae in Australia, with zoogeographical considerations

Molecular and pathological studies on Angiostrongylus species in southeast Queensland

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