Experimental Hematogenous Osteomyelitis by Staphylococcus Aureus

Matsushita, Kazunobu; Hamabe, Masaki; Matsuoka, Motohiro; Aoki, Haruhito; Miyoshi, Kunisato; Ichiman, Yoshitoshi; Shimada, Jingoro

doi:10.1097/00003086-199701000-00038

Cited by 12 publications

(5 citation statements)

References 14 publications

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“…3 Although the prevalence of acute hematogenous osteomyelitis and septic arthritis has remained unchanged for the past 20 years, the causative organisms have changed dramatically. 9 Within minutes to hours of microbial implantation, bacterial adhesion occurs and infection develops. [3][4][5][6] PATHOPHYSIOLOGY Acute hematogenous osteomyelitis and septic arthritis develop similarly.…”

Section: Definition and Prevalencementioning

confidence: 99%

Managing musculoskeletal infections in children in the era of increasing bacterial resistance

Godley

2015

Journal of the American Academy of Physician Assistants

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Section: Definition and Prevalencementioning

confidence: 99%

Managing musculoskeletal infections in children in the era of increasing bacterial resistance

Godley

2015

Journal of the American Academy of Physician Assistants

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“…58 This study established the basis for the creation of contained osteomyelitis models and led to the development of a number of models based on intramedullary injection of sclerosing agents. 4 Sclerosing agents are used to facilitate the formation of bone infections, because the agents result in sclerosis of the vessels in the 66,[67][68][69][70][71][72][73][74] • Economical • Fast inoculation procedure • Tolerate broad-spectrum antibiotic therapy…”

Section: Rabbit Modelsmentioning

confidence: 99%

“…Other publications using mice exposed to only intravenous injection of bacteria for development of HO without artificial trauma have aimed at testing the virulence of different S. aureus strains. [70][71][72][73] In these studies, only histopathological bone lesions have been reported with no information regarding examination for, eg, systemic side effects or the presence of nonosseous lesions. [70][71][72] However, in a recently published paper, mice exposed to tail vein inoculation survived for 56 days and developed chronic HO, reproducing most features of the human disease.…”

Section: Mouse Modelsmentioning

confidence: 99%

“…59 Rabbits of 8 weeks were also used in the models developed by Morrisy and Haynes 24 and Whalen et al 60 The murine models have been based on female mice that were 8-to 10-weeks-old. 66,[68][69][70][71][72][73][74] Experimental mice are weaned and sexually mature at this age, which might influence the inability to establish large macroscopic lesions in most of these models. 66,[68][69][70][71][72][73] The porcine 30,75 and avian [63][64][65] HO models were developed in growing animals and demonstrated the presence of transphyseal vascular tunnels, which are comparably found in infants.…”

Section: Route Of Inoculationmentioning

confidence: 99%

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Animal models of hematogenous Staphylococcus aureus osteomyelitis in long bones: a review

Johansen

Jensen

2013

ORR

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Hematogenous osteomyelitis (HO), especially due to Staphylococcus aureus, is primarily reported in children and occurs when blood-borne bacteria settle in the metaphysis of a long bone and mediate an inflammatory response. The literature contains several reports on animal models aiming to simulate pediatric HO, in order to investigate the pathogenesis and for therapeutic use. In these models, osteomyelitis lesions develop subsequently to bacteremia, which can be induced by either intravenous or intra-arterial inoculation of bacteria. Intravenous inoculation is not optimal because of the ethical aspects of the extensive systemic reaction and the unpredictable identity of bones being infected. Also, intravenous inoculation often has to be combined with the induction of artificial bone necrosis in order to have macroscopic lesions. In contrast, models based on intra-arterial inoculation and subsequent development of local osteomyelitis, are the most accurate and predictable way to extrapolate to pediatric cases of HO. The most commonly used animal species for modeling of HO are rabbits, chickens, and mice, whereas, less frequently, dogs, rats, and pigs have been applied. The use of intra-arterial inoculation, without simultaneous artificial bone necrosis for the development of HO lesions has only been used in porcine models. Because of the similarity of human and porcine physiology, metabolic rate, and size, porcine models of HO are advantageous. Therefore, porcine models based on the intra-arterial induction of osteomyelitis are the most refined HO models.

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“…sections (Matsushita et al, 1997). To determine if L. monocytogenes caused similar lesions, we excised leg bones from mice displaying BLI signals (not shown) for image-guided histology (Fig.…”

Section: Staphylococcus Aureus Causes Granulomatous Bone Marrow Lesions and Accompanying Pathology That Are Very Apparent In Histologicalmentioning

confidence: 99%

Translational Impact

2009

Disease Models &Amp; Mechanisms

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Murine listeriosis is one of the most comprehensive and well-studied models of infection, and Listeria monocytogenes has provided seminal information regarding bacterial pathogenesis. However, many aspects of the mouse model remain poorly understood, including carrier states and chronic colonization which represent important features of the spectrum of host-pathogen interaction. Bone marrow has recently been shown to harbor L. monocytogenes, which spreads from this location to the central nervous system. Bone could, therefore, be an important chronic reservoir, but this infection is difficult to study because it involves only a few bacteria and the extent of infection cannot be assessed until after the animal is sacrificed. We employed in vivo bioluminescence imaging to localize L. monocytogenes bone infections over time in live mice, revealing that the bacteria grow in discrete foci. These lesions can persist in many locations in the legs of mice and are not accompanied by a histological indication such as granuloma or a neutrophil infiltratate. We demonstrate that highly attenuated hly mutants, which have defective intracellular replication, are capable of prolonged focal infection of the bone marrow for periods of up to several weeks. These results support the recently proposed hypothesis that the bone marrow is a unique niche for L. monocytogenes.

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Experimental Hematogenous Osteomyelitis by Staphylococcus Aureus

Cited by 12 publications

References 14 publications

Managing musculoskeletal infections in children in the era of increasing bacterial resistance

Managing musculoskeletal infections in children in the era of increasing bacterial resistance

Animal models of hematogenous Staphylococcus aureus osteomyelitis in long bones: a review

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