Experimental full-thickness burns induced by CO2 laser

Melo, Milene da Silva; Alves, Leandro Procópio; Navarro, Ricardo Scarparo; Lima, Carlos José de; Munin, Egberto; Vilela-Goulart, Maria das Graças; Gomes, Mônica Fernandes; Salgado, Miguel Angel Castillo; Zângaro, Renato Amaro

doi:10.1007/s10103-014-1585-5

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…A CO 2 laser (Synrad Model J48-5-2848, USA) with wavelength of 10.600 nm in continuous mode was used to induce three full-thickness burns in each animal according to a previously published protocol [19]. The optical power was 1.3 W; the laser beam diameter was 3.5 mm and the irradiated surface was 0.096 cm 2 .…”

Section: Induction Of Burnsmentioning

confidence: 99%

LED phototherapy in full-thickness burns induced by CO2 laser in rats skin

et al. 2018

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Many studies have been conducted on the treatment of burns because they are important in morbidity and mortality. These studies are mainly focused on improving care and quality of life of patients. The aim of this study was evaluate the LED phototherapy effects in rats skin full-thickness burns induced by CO laser. The animals were divided in NT group that did not received any treatment and LED group that received LED irradiation at 685 nm, 220 mW, and 4.5 J/cm during 40 s by burned area. Biopsies were obtained after 7, 14, and 21 days of treatment and submitted to histological and immunohistochemical analysis. The LED phototherapy shows anti-inflammatory effects, improves angiogenesis, and stimulates the migration and proliferation of fibroblasts. The T CD8+ lymphocytes were more common in burned areas compared to T CD4+ lymphocytes since statistically significant differences were observed in the LED group compared to the NT group after 7 days of treatment. These results showed that LED phototherapy performs positive influence in full-thickness burns repair from the healing process modulated by cellular immune response. The obtained results allowed inferring that burns exhibit a characteristic cell immune response and this cannot be extrapolated to other wounds such as incision and wounds induced by punch, among others.

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Section: Induction Of Burnsmentioning

confidence: 99%

LED phototherapy in full-thickness burns induced by CO2 laser in rats skin

et al. 2018

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“… 11 Contact injury models [heated aluminum, 12–16 heated brass or steel, 11 , 17–26 electricity 27 , 28 ] are relatively affordable but require complex technical setups, thus making the size, shape and depth of the burns difficult to standardize. Some models have been created using lasers of various types: laser light (Thulium CW laser, 29 CO 2 laser, 30 , 31 mid-infrared diode laser, 32 blue beam laser, 33 pulsed dye laser (PDL). 8 , 34 , 35 …”

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confidence: 99%

A New Ex Vivo Human Skin Burn Model

Labouchère

Haselbach

Michetti

et al. 2023

Journal of Burn Care &Amp; Research

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Currently, most burn models for preclinical testing are on animals. For obvious ethical, anatomical, and physiological reasons, these models could be replaced with optimized ex vivo systems. The creation of a burn model on human skin using a pulsed dye laser could represent a relevant model for preclinical research. Six samples of excess human abdominal skin were obtained within one hour after surgery. Burn injuries were induced on small samples of cleaned skin using a pulsed dye laser on skin samples, at varying fluences, pulse numbers and illumination duration. In total, 70 burn injuries were performed on skin ex vivo before being histologically and dermato-pathologically analyzed. Irradiated burned skin samples were classified with a specified code representing burn degrees. Then, a selection of samples was inspected after 14 and 21 days to assess their capacity to heal spontaneously and re-epithelize. We determined the parameters of a pulsed dye laser inducing 1 st, 2 nd and 3 rd degree burns on human skin and with fixed parameters, especially superficial and deep 2 nd degree burns. After 21 days with the ex vivo model, neo-epidermis was formed. Our results showed that this simple, rapid, user-independent process creates reproducible and uniform burns of different, predictable degrees that are close to clinical reality. Human skin ex vivo models can be an alternative to and complete animal experimentation, particularly for preclinical large screening. This model could be used to foster the testing of new treatments on standardized degrees of burn injuries and thus improve therapeutic strategies.

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Analysis of experimental tendinitis in rats treated with laser and platelet-rich plasma therapies by Raman spectroscopy and histometry

et al. 2015

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The objective of this controlled experimental study was to analyze the changes in the Achilles tendons of rats with experimentally induced tendinitis after treatment with platelet-rich plasma (PRP) and/or laser therapies by histometry to quantify fibroblasts and by Raman spectroscopy to determine the biochemical concentration of collagen types I and III. Fifty-four male Wistar rats were divided into six treatment groups: control (G1); PRP only (G2); irradiation with 660 nm laser (G3); irradiation with 830 nm laser (G4); PRP plus 660 nm laser irradiation (G5); and PRP plus 830 nm laser irradiation (G6). Injuries (partial tenotomy) were inflicted in the middle third of the Achilles tendon, with PRP added prior to suture in the appropriate experimental groups. A diode laser (model Laser Flash® III, DMC Equipamentos Ltda, São Carlos, SP, Brazil) that can be operated in two wavelengths 660 and 830 nm was used for irradiation treatments. The irradiation protocol was energy density of 70 J/cm², 20 s irradiation time, and 0.028 cm² spot area, per point in three points in the injured. The histometry was made in micrographical images of the H&E stained sections and evaluated by ImageJ (version 1.46r)®. Raman spectra were collected using a dispersive spectrometer at 830 nm excitation, 200 mW power, and 10 s integration time (P-1 Raman system, Lambda Solutions, Inc. MA, USA). The relative amount of type I collagen was significantly greater in the PRP plus 830 nm laser irradiation group (468 ± 188) than in the control (147 ± 137), 630 nm laser only (191 ± 117), and 830 nm laser only (196 ± 106) groups (p < 0.01), while the quantity of type III collagen was significantly greater in the PRP-only group compared to both irradiated groups without PRP (p < 0.05). Treatment with PRP combined with irradiation at 830 nm resulted in a larger number of fibroblasts and increased concentration of type I collagen, thus accelerating the healing of the injured tendon.

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Experimental full-thickness burns induced by CO2 laser

Cited by 4 publications

References 24 publications

LED phototherapy in full-thickness burns induced by CO2 laser in rats skin

LED phototherapy in full-thickness burns induced by CO2 laser in rats skin

A New Ex Vivo Human Skin Burn Model

Analysis of experimental tendinitis in rats treated with laser and platelet-rich plasma therapies by Raman spectroscopy and histometry

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