Experimental Evidences on Synergy of Gas Discharge Agents in Bactericidal Activity of Nonthermal Plasma

Sysolyatina, Elena V.; Mukhachev, Andrey; Yurova, Maria; Грушин, М. Е.; Karalnik, Vladimir; Petryakov, A. V.; Трушкин, Н. И.; Данилова, М. Н.; Naroditsky, Boris S.; Gintsburg, A. L.; Akishev, Yurii S.; Sа, Ermolaeva

doi:10.1615/plasmamed.2014008194

Cited by 1 publication

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Untreated DI water retained a conductivity of 0 mS/cm 3 , even after 24 hours. The absence of change in the perturbations introduced in the conductivity of DI water post treatment showed that the conductivity created is a consequence of oxygen and nitrogen species rather than ions, which have a life span on the order of nanoseconds to milliseconds 41 , 49 . Therefore, the effects of ions in aqueous environments may be discounted as playing an important role.…”

Section: Resultsmentioning

confidence: 99%

Investigation of the Roles of Plasma Species Generated by Surface Dielectric Barrier Discharge

Pai

Timmons

Roehm

et al. 2018

Sci Rep

View full text Add to dashboard Cite

As an emerging sterilization technology, cold atmospheric plasma offers a dry, non-thermal, rapid process that is minimally damaging to a majority of substrates. However, the mechanisms by which plasma interacts with living cells are poorly understood and the plasma generation apparatuses are complex and resource-intensive. In this study, the roles of reactive oxygen species (ROS), nitric oxide (NO), and charged particles (ions) produced by surface dielectric barrier discharge (SDBD) plasma on prokaryotic (Listeria monocytogenes (Gram-positive)) and eukaryotic (human umbilical vein endothelial cells (HUVEC)) cellular function were evaluated. HUVEC and bacterial oxidative stress responses, the accumulation of nitrite in aqueous media, air ion density, and bacterial inactivation at various distances from SDBD actuators were measured. SDBD actuator designs were also varied in terms of electrode number and length to evaluate the cellular effects of plasma volume and power distribution. NO and ions were found to contribute minimally to the observed cellular effects, whereas ROS were found to cause rapid bacterial inactivation, induce eukaryotic and prokaryotic oxidative stress, and result in rapid oxidation of bovine muscle tissue. The results of this study underscore the dominance of ROS as the major plasma generated species responsible for cellular effects, with ions and RNS having a secondary, complimentary role.

show abstract

Section: Resultsmentioning

confidence: 99%