Experimental evidence of the electrostatic contribution to membrane bending rigidity

Rowat, Amy C.; Hansen, Per Lyngs; Ipsen, John Hjort

doi:10.1209/epl/i2003-10276-x

Cited by 38 publications

(27 citation statements)

References 40 publications

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“…Based on the comparison between the experimental data and the computer simulations, we consider that these vesicles have reached equilibrium, and electrostatic forces are balanced with the gravitational force. These results suggest that the presence of charged fluorescent probes does have the effect of increasing the bending rigidity as has been reported previously [59].…”

Section: Vesicle Deformation At Equilibriumsupporting

confidence: 88%

“…5) suggests that strain at a certain tension depends on the membrane bending rigidity. It has been suggested previously [59][60][61] that electrostatic interactions between neighboring NBD-PE molecules should provide a contribution to the bending rigidity. In order to verify this observation we compare the strain of charged and uncharged vesicles.…”

Section: Vesicle Deformation At Equilibriummentioning

confidence: 94%

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Slow Sedimentation and Deformability of Charged Lipid Vesicles

et al. 2013

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The study of vesicles in suspension is important to understand the complicated dynamics exhibited by cells in in vivo and in vitro. We developed a computer simulation based on the boundary-integral method to model the three dimensional gravity-driven sedimentation of charged vesicles towards a flat surface. The membrane mechanical behavior was modeled using the Helfrich Hamiltonian and near incompressibility of the membrane was enforced via a model which accounts for the thermal fluctuations of the membrane. The simulations were verified and compared to experimental data obtained using suspended vesicles labelled with a fluorescent probe, which allows visualization using fluorescence microscopy and confers the membrane with a negative surface charge. The electrostatic interaction between the vesicle and the surface was modeled using the linear Derjaguin approximation for a low ionic concentration solution. The sedimentation rate as a function of the distance of the vesicle to the surface was determined both experimentally and from the computer simulations. The gap between the vesicle and the surface, as well as the shape of the vesicle at equilibrium were also studied. It was determined that inclusion of the electrostatic interaction is fundamental to accurately predict the sedimentation rate as the vesicle approaches the surface and the size of the gap at equilibrium, we also observed that the presence of charge in the membrane increases its rigidity.

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Section: Vesicle Deformation At Equilibriumsupporting

confidence: 88%

Section: Vesicle Deformation At Equilibriummentioning

confidence: 94%

Slow Sedimentation and Deformability of Charged Lipid Vesicles

et al. 2013

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“…Experimental data, albeit limited, 22 in general support the theoretical results. Of particular interest is the variation of the bending rigidity with surface charge, however, this problem has been investigated either only for relatively low fractions of the ionic species (lipids or surfactants) [23][24][25][26][27][28] or on purely ionic lyotropic systems. 29,30 Giant unilamellar vesicles (GUVs) represent a suitable system for systematic measurements on membranes with good control of the composition.…”

Section: Introductionmentioning

confidence: 99%

Bending rigidity of charged lipid bilayer membranes

et al. 2019

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“…Although not extensively studied, surface electrostatics can also have profound influences on the membrane structural dynamics through variations in elasticity and in the membrane bending rigidity having consequences on the deformability induced by surfactants and peptides (Rowat et al 2004;Böckmann et al 2003;Kmetto et al 2001). Membranes may become unstable from long-wavelength undulations due to Coulomb repulsion between excess charges which affect the membrane bending rigidity.…”

Section: Effect Of Electrostatics On Membrane Rheologymentioning

confidence: 99%

Electrostatic field effects on membrane domain segregation and on lateral diffusion

Wilke

Maggio

2011

Biophys Rev

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Natural membranes are organized structures of neutral and charged molecules bearing dipole moments which generate local non-homogeneous electric fields. When subjected to such fields, the molecules experience net forces that can modify the lipid and protein organization, thus modulating cell activities and influencing (or even dominating) the biological functions. The energetics of electrostatic interactions in membranes is a long-range effect which can vary over distance within r −1 to r −3 . In the case of a dipole interacting with a plane of dipoles, e.g. a protein interacting with a lipid domain, the interaction is stronger than two punctual dipoles and depends on the size of the domain. In this article, we review several contributions on how electrostatic interactions in the membrane plane can modulate the phase behavior, surface topography and mechanical properties in monolayers and bilayers.

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Experimental evidence of the electrostatic contribution to membrane bending rigidity

Cited by 38 publications

References 40 publications

Slow Sedimentation and Deformability of Charged Lipid Vesicles

Slow Sedimentation and Deformability of Charged Lipid Vesicles

Bending rigidity of charged lipid bilayer membranes

Electrostatic field effects on membrane domain segregation and on lateral diffusion

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