Experimental Evaluation of the Chemotherapeutic Efficacy and Toxicity of a New Medicinal Form of Dioxidine: Lidoxycol Ointment

Pushkina, T. V.; Krylova, L. Yu.; Sharova, S. A.; Chicherina, L. A.; Kuzina, O.S.

doi:10.1007/s11094-008-0141-7

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“…Dioxidine has been used in Russia for nearly 30 years to treat purulent infections, can be applied topically or injected intravenously into body cavities, and was the only well-characterized hit compound from a commercial library that inhibited E. coli 0157:H7 growth. Dioxidine's mode of action involves interference with DNA synthesis (28). Recent research has focused on potential applications of dioxidine, ranging from an antituberculosis cocktail to antimicrobial topical ointments (14,28).…”

Section: Most Compounds That Inhibited E Coli 0157:h7 Growth In Vitrmentioning

confidence: 99%

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High-Throughput Small Molecule Screening Reveals Structurally Diverse Compounds That Inhibit the Growth of Escherichia coli O157:H7 In Vitro

Chen

Carlson

Sofos

et al. 2011

Journal of Food Protection

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Escherichia coli O157:H7 colonizes the gastrointestinal tract of ruminants asymptomatically and may enter the human food supply through fecal contamination. A fraction of individuals infected by E. coli O157:H7 develop hemolytic uremic syndrome, a life-threatening condition. When individuals infected by E. coli O157:H7 are treated with certain antibiotics, an increased incidence of hemolytic uremic syndrome may result. This finding supports the need to identify novel compounds that can either reduce the load of E. coli O157:H7 entering the human food supply or serve as alternative therapeutic treatments for infected individuals. We developed a high-throughput turbidometric assay to identify novel compounds that inhibit E. coli O157:H7 growth. Pin transfers were performed to introduce small molecule libraries into 384-well plates, where each well contained approximately 5.0 log CFU of E. coli O157:H7. Plates were incubated at 37°C for 18 h, and the optical density was measured to determine the effect of each small molecule. A total of 64,562 compounds were screened in duplicate, and 43 unique compounds inhibited E. coli O157:H7 growth. Thirty-eight of the 43 inhibitory compounds belonged to known bioactive libraries, and the other 5 compounds were from commercial libraries derived from splitting and pooling. Inhibitory compounds from known bioactive libraries were most frequently therapeutic antibiotics (n = 34) but also included an antiviral compound, a compound that disrupts the citric acid cycle, and two biguanide compounds, which have been used for various nonclinical applications. We identified two novel compounds (i.e., biguanides) that should be studied further for their ability to reduce pathogen populations in foods.

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Section: Most Compounds That Inhibited E Coli 0157:h7 Growth In Vitrmentioning

confidence: 99%

“…Dioxidine's mode of action involves interference with DNA synthesis (28). Recent research has focused on potential applications of dioxidine, ranging from an antituberculosis cocktail to antimicrobial topical ointments (14,28).…”

Section: Most Compounds That Inhibited E Coli 0157:h7 Growth In Vitrmentioning

confidence: 99%

High-Throughput Small Molecule Screening Reveals Structurally Diverse Compounds That Inhibit the Growth of Escherichia coli O157:H7 In Vitro

Chen

Carlson

Sofos

et al. 2011

Journal of Food Protection

View full text Add to dashboard Cite

show abstract

Experimental Evaluation of the Chemotherapeutic Efficacy and Toxicity of a New Medicinal Form of Dioxidine: Lidoxycol Ointment

Cited by 1 publication

References 0 publications

High-Throughput Small Molecule Screening Reveals Structurally Diverse Compounds That Inhibit the Growth of Escherichia coli O157:H7 In Vitro

High-Throughput Small Molecule Screening Reveals Structurally Diverse Compounds That Inhibit the Growth of Escherichia coli O157:H7 In Vitro

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