Experimental evaluation and computational modeling of tissue damage from low-flow push–pull perfusion sampling in vivo

Cepeda, David E.; Hains, Leah E.; Li, David; Bull, Joseph L.; Lentz, Stephen I.; Kennedy, Robert T.

doi:10.1016/j.jneumeth.2015.01.019

Cited by 18 publications

(22 citation statements)

References 32 publications

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“…It is however even more specialized than microdialysis. Another push-pull technique was studied regarding possible tissue damage, with the conclusion that push-pull perfusion at low flow rates works well for sampling from brain, resulting in high temporal and spatial resolution, and that the probe insertion is what causes the tissue damage (99).…”

Section: Development Of Microdialysis and Alternativesmentioning

confidence: 99%

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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Abstract.Microdialysis has contributed with very important knowledge to the understanding of target-specific concentrations and their relationship to pharmacodynamic effects from a systems pharmacology perspective, aiding in the global understanding of drug effects. This review focuses on the historical development of microdialysis as a method to quantify the pharmacologically very important unbound tissue concentrations and of recent findings relating to modeling microdialysis data to extrapolate from rodents to humans, understanding distribution of drugs in different tissues and disease conditions. Quantitative microdialysis developed very rapidly during the early 1990s. Method development was in focus in the early years including development of quantitative microdialysis, to be able to estimate true extracellular concentrations. Microdialysis has significantly contributed to the understanding of active transport at the blood-brain barrier and in other organs. Examples are presented where microdialysis together with modeling has increased the knowledge on tissue distribution between species, in overweight patients and in tumors, and in metabolite contribution to drug effects. More integrated metabolomic studies are still sparse within the microdialysis field, although a great potential for tissue and disease-specific measurements is evident.

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Section: Development Of Microdialysis and Alternativesmentioning

confidence: 99%

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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“…Attempts to modify this technology led to the development of push-pull probes (PPP), which enable faster analysis, but lower sensitivity and accuracy 26 . Compared to MD and SPME, PPPs and electrochemical methods guarantee better temporal resolution, although pushpull probes are still prone to capillary clogging 26,27 . Additionally, while electrochemical methods are able to return results in a few seconds, they only enable the analysis of selected substances, which may considerably reduce the scope of research 26,28 .…”

Section: Discussionmentioning

confidence: 99%

SPME Probes: A Novel Chemical Biopsy Tool for Spatially Resolved Profiling of Human Brain Tissue in vivo

Bojko

Bogusiewicz

Burlikowska

et al. 2021

Preprint

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It is extremely challenging to perform chemical analyses of the brain, particularly in humans, due to the restricted access to this organ. Imaging techniques are the primary approach used in clinical practice, but they only provide limited information about brain chemistry. Solid-phase microextraction (SPME) has been presented recently as a chemical biopsy tool for the study of animal brains. The current work demonstrates for the first time the use of SPME for the spatially resolved sampling of the human brain in vivo. Specially designed multi-probe sampling device was used to simultaneously extract metabolites from the white and grey matter of patients undergoing brain tumor biopsies. Samples were collected by inserting the probes along the planned trajectory of the biopsy needle prior to the procedure, which was followed by metabolomic and lipidomic analyses. The results revealed that studied brain structures were predominantly composed of lipids, while the concentration and diversity of detected metabolites was higher in white than in grey matter. Although the small number of participants in this research precluded conclusions of a biological nature, the results highlight the advantages of the proposed SPME approach, as well as disadvantages that should be addressed in future studies.

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“…Others laser etch holes into existing catheters to simplify the fabrication process [38]. However, microperfusion probes require a secondary pull pump to ensure a zero net fluctuation of fluid from the probe into the interstitial space [33,34,39]. Open flow microperfusion (OFM) has been validated in several studies in human skeletal muscle [40], adipose tissue [40], and cerebral tissue (cOFM) [21], supporting the validity of the approach in vivo.…”

Section: Mwco-b Mw-amentioning

confidence: 96%

“…Factors that skew the collection of biomarkers due to tissue damage are the size [48,57], geometry [58], and rigidity [59] of the implanted probe. The volume of damaged tissue increases with the cross-sectional size of the probe [39]. The cross-sectional geometry of the probe also plays a role in the variety and population of collected biomarkers [58][59][60].…”

Section: Invasive Consequences Of Implantation and Biofoulingmentioning

confidence: 99%

Microdialysis and microperfusion electrodes in neurologic disease monitoring

Stangler

Kouzani

Bennet

et al. 2021

Fluids Barriers CNS

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Contemporary biomarker collection techniques in blood and cerebrospinal fluid have to date offered only modest clinical insights into neurologic diseases such as epilepsy and glioma. Conversely, the collection of human electroencephalography (EEG) data has long been the standard of care in these patients, enabling individualized insights for therapy and revealing fundamental principles of human neurophysiology. Increasing interest exists in simultaneously measuring neurochemical biomarkers and electrophysiological data to enhance our understanding of human disease mechanisms. This review compares microdialysis, microperfusion, and implanted EEG probe architectures and performance parameters. Invasive consequences of probe implantation are also investigated along with the functional impact of biofouling. Finally, previously developed microdialysis electrodes and microperfusion electrodes are reviewed in preclinical and clinical settings. Critically, current and precedent microdialysis and microperfusion probes lack the ability to collect neurochemical data that is spatially and temporally coincident with EEG data derived from depth electrodes. This ultimately limits diagnostic and therapeutic progress in epilepsy and glioma research. However, this gap also provides a unique opportunity to create a dual-sensing technology that will provide unprecedented insights into the pathogenic mechanisms of human neurologic disease.

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Experimental evaluation and computational modeling of tissue damage from low-flow push–pull perfusion sampling in vivo

Cited by 18 publications

References 32 publications

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

SPME Probes: A Novel Chemical Biopsy Tool for Spatially Resolved Profiling of Human Brain Tissue in vivo

Microdialysis and microperfusion electrodes in neurologic disease monitoring

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