Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability

Silva, Eduardo Moreira da; Rodrigues, Carolina Ullmann Fernandes de Sá; Matos, Marcos Paulo de Oliveira; Carvalho, Thais Rodrigues de; Santos, Georgina Silva dos; Amaral, Cristiane Mariote

doi:10.1016/j.jdent.2015.10.004

Cited by 31 publications

(43 citation statements)

References 38 publications

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“…The application of MMP inhibitors on the dentin surface as an additional step for dentin treatment could result in the reduction of bond strength loss and the improvement of restoration stability. 5 CHX has been widely used as a broad-spectrum MMP inhibitor to improve the bond strength to dentin. Nevertheless, CHX exhibited cytotoxicity to oral cells, which was consistent with the findings that CHX inhibited cell growth of MDPC-23.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 MMP inhibition offered an effective approach to preserving the mechanical properties of composite resin-dentin bonding. 4,5 Biomimetic dentin remineralization, a valuable tool for maintaining the mechanical properties and biostability of collagen fibrils, is an emerging biomedical technology for improving the durability of the bond strength. [6][7][8] Remineralization of demineralized dentin may reduce collagen degradation, [9][10][11] and specific agents have been used for this purpose.…”

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confidence: 99%

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Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

Xie

Cao

et al. 2019

The Journal of Prosthetic Dentistry

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Statement of problem. Polyvinylphosphonic acid (PVPA) could be used as a biomimetic remineralization analog and a matrix metalloproteinases (MMPs) inhibitor. However, studies are lacking regarding the performance of PVPA in dental bonding systems for maintaining the durability of the resin-dentin bond.Purpose. The purpose of this in vitro study was to investigate the effect of PVPA on the durability of resin-dentin bonds and the viability of mouse dental papilla cell-23 (MDPC-23). The mechanical properties of resin-dentin interfaces during long-term storage were analyzed, and the potential application of PVPA as a biomimetic remineralization analog in adhesive dentistry was evaluated.Material and methods. Seventy-five extracted noncarious human third molars were collected and randomly divided into 5 groups, and then the microtensile bond strength (mTBS) data and scanning electron microscope (SEM) images were used to evaluate the preservation condition of resin-dentin bonds after 1 day, 6 months, and 1 year of storage. The cytotoxicity of PVPA was detected by cell proliferation assay and cell apoptosis assay.Results. Compared with the control and chlorhexidine (CHX) groups, the combined group (treated with both 200-mg/mL PVPA and biomimetic remineralization) had excellent bond durability. The exposed collagen fibril from the PVPA-treated groups (included 200-mg/mL and 500-mg/mL PVPA groups and a combined group) still showed integrity after 1 year of storage when compared with the control group. PVPA up to 500 mg/mL showed no cytotoxicity to MDPC-23 and did not inhibit cell growth.Conclusions. This study offered evidence that PVPA did not result in cytotoxicity at low concentrations as an MMP inhibitor and a biomimetic remineralization analog. In addition, the application of PVPA improved bond strength and preserved collagen integrity after 1 year of in vitro storage. (J Prosthet Dent 2019;122:492.e1-e6) 492.e1 THE JOURNAL OF PROSTHETIC DENTISTRY

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

Xie

Cao

et al. 2019

The Journal of Prosthetic Dentistry

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“…Restoratif işlemler sırasında MMP enzimlerinin ortaya çıkması, dentin-adeziv bağlanmasında başarısızlık sebebi olarak görülmektedir. 20,21 Nishitani ve ark. yaptıkları in vitro çalışmada, dentine adeziv uygulanmasının ardından tam olarak örtülemeyen hibrit tabakasındaki kollajen fibrillerin bozulduğunu veya yok olduğunu göstermişlerdir.…”

Section: Adeziv Bağlanmanın Bozulmasıunclassified

Effects of matrix metalloproteinase enzyms on adhesive bonding

Halaçoğlu¹,

Can²

2017

Yeditepe J Dent

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ÖZETMatriks metalloproteinaz enzimleri (MMP), dentin matriksinin içinde bulunan proteolitik enzimlerdir ve dentinin organik matriksini hidrolize etme özellikleri vardır. Rezin-dentin bağlanmasının elde edilmesi için dentine asit uygulanması sonrası dentinin kollajen fibrilleri açığa çıkar. Bu açığa çıkan kollajen fibriller MMP'lerin başlattığı hidrolitik bozulmadan etkilenebilir ve bunun sonucunda bağlanma dayanımında azalma olarak restorasyonların ağız içerisindeki uzun dö-nem performansı negatif etkilenebilir. Bu derlemenin amacı, dentin kaynaklı MMP'lerin dentin matriks bozulmasındaki rolünün anlaşılması ve kollajen bozulmasını inhibe eden MMP inhibitörlerinin dentinin bağlanma dayanımına etkisinin özetlenmesidir. Anahtar Kelimeler: Matriks metalloproteinazlar, dental adezivler, kollajen SUMMARY Matrix metalloproteinase enzymes (MMP) are proteolytic enzymes that found in dentin matrix and they are capable to hydrolyze organic matrix of dentin. Dentin collagen fibrils are exposed after acid etching of dentin in order to achieve resin-dentin bonding. These exposed collagen fibrils might be effected by hydrolytic degradation caused by MMP's resulting reduced bonding strength and long-term performance in oral cavity. The purpose of this review is to understand the role of MMP's in dentin matrix degradation and to summarize the effects of MMP inhibitors that inhibits the collagen degradation to dentin bonding strength. Key words: Matrix metalloproteinases, dental adhesives, collagen GİRİŞ Restoratif diş hekimliği alanında yapılan pek çok çalışma ve yeni geliştirilen materyallere rağmen, dentin-adeziv ara yüzeyi diş restorasyon kompleksindeki en zayıf nokta olma özelliğini korumaktadır. Rezin-dentin bağlanmasının elde edilmesi için dentin yüzeyinin fiziksel ve kimyasal özelliklerini değiştirecek birkaç uygulama yapılması gerekir. Kavite preparasyonu sırasında dentin yüzeyinde oluşan 1-2µm kalınlığında smear tabakası self-etching primer ile modifiye edilir veya %37'lik fosforik asit ile tamamen ortadan kaldırılır.1 Dentinin asitlendiği durumlarda, dentin yüzeyinde-ki tüm mineral komponent ve smear tabakasının altında bulunan 5µm kalınlıkta mineralize dentin çözünür ve böylece altta bulunan tip I kollajen fibril ağı açığa çıkar.2 Kollajen fibriller arasında oluşan boşluğa interfibriler alan adı verilir ve bu boşluk adeziv komonomerleri için difüzyon kanalları oluşturur.3 Adeziv komonomerleri demineralizasyon derinliği boyunca ilerleyerek hibrit tabakasını oluşturur. 4 İdealde hibrit tabakasının uzun yıllar boyunca sağlam kalması beklenir, ancak zamanla hidrolitik çözünmeden dolayı adeziv ara yüzey-de bozulmalar meydana gelebilir.

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“…MMPs are a Ca+ and Zn+ dependent enzyme family capable of degrading various components of the extracellular organic matrix. These enzymes, synthesized and secreted by odontoblasts [90] and activated during adhesive procedures [17], deteriorate the exposed collagen fibrils at the base of the hybrid layer by both etch-and-rinse or self-etching adhesives [17,91]. To decrease degradation, MMP inhibitors are used and a meta-analysis ( Figure 6) was developed to compare the effectiveness of these inhibitors.…”

Section: Article 49mentioning

confidence: 99%

“…The main endogenous MMPs of dentin are collagenase-2 (MMP-8), gelatinase-B (MMP-9) [13,14], and gelatinase-A (MMP-2) [13,14], which belong to the family of calcium and zincdependent proteolytic enzymes [15] found most frequently in dentin tissue affected by caries [15,16]. In its latent form, these MMPs are not able to degrade collagen; however, after the pH decrease during acid conditioning this MMPs is activated and followed by neutralization are able causing degradation of exposed collagen fibrils [15,17]. Thus, exposed collagen fibrils lead to decreased bond strength and cohesive failures in demineralized dentin below the hybrid layer, both in etch-and-rinse and self-etching adhesives [18,19].…”

Section: Introductionmentioning

confidence: 99%

Influence of MMP inhibitors on bond strength of adhesive restorations: systematic review and meta-analysis

Camim¹

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A influência de inibidores de MMP na resistência de união de restaurações adesivas: revisão sistemática e metanálise BAURU 2019 Orientador: Prof. Dr. Heitor Marques Honório FOLHA DE APROVAÇÃO DEDICATÓRIA Dedico esse trabalho á Deus que me sustentou nos momentos mais difíceis e a minha família, em especial meus pais, minha irmã e meus avós que me apoiaram durante essa trajetória. AGRADECIMENTOS GERAIS A DeusAgradeço primeiramente a Deus por ter me dado força e coragem pra chegar até aqui, sempre com sorriso no rosto e acreditando que o melhor aconteceria. A famíliaA minha mãe Cristiane e ao meu pai Valmir por me apoiarem sempre que eu precisei, por serem o meu alicerce, por entenderem meu sonho e nunca soltarem a minha mão.Á você minha irmã Nathielly que mesmo tão diferente de mim sempre me entende e me ajuda tão bem, obrigada por tudo. Aos meus avós Devanir e Waldomiro sempre com a simplicidade e corações gigantes pra me ajudar, amo vocês. Aos colegas de mestradoAos meus colegas de mestrado 2017-2019, Daniella, Juliana, Naoki, Genine, Edgar, Mariele, Leandro, Alyssa, Victor e Camila Queiroz, obrigada por serem minha família durante esses anos, por serem meu suporte nas horas que precisei, por dividirem comigo suas experiências, por me mostrarem que apesar de todas as diferenças podemos um ajudar o outro, por dividirem comigo as horas de estresse, raiva e felicidade.Em especial a você Juliana por dividir comigo todos meus medos, fraquezas e momentos de alegria, levarei você dentro do meu coração e tenho certeza que sucesso é certo. Aos amigosAgradeço o apoio de todos meus amigos que de forma direta e indireta estiveram do meu lado e acreditaram que este dia chegaria, em especial vocês Aélem, Natália, Filipe, Gabriela, Raul, Géssica e Carol, obrigada por tudo. Ao meu orientadorAo meu orientador Heitor que acreditou no meu potencial desde que cheguei na Faculdade de Odontologia de Bauru, que me auxiliou e me deu oportunidades que eu jamais imaginei. Você sempre paciente, amigo, um pai para seus orientados, eu não podia ter tido sorte maior. Que esses seja o começo de muito trabalho, que eu possa sempre encontrar as portas abertas como foi esses dois anos. Ao meu mentorAo meu eterno orientador e mentor Rafael Maenosono porque sem o seu auxilio eu não estaria onde estou, você confiou em mim mesmo quando eu mesma duvidava, obrigada pelos conselhos e oportunidades, por confiar em mim todos esses anos. Eu serei eternamente grata por tudo. Ao Centro Universitário de Santa Fé do Sul -UNIFUNEC, por ter me proporcionada toda a base profissional que me fez chegar aqui, além do apoio de todos meus professores, em especial Max Douglas e Érica Delalibera. , que transmitiram seus conhecimentos de forma direta ou indireta para que eu pudesse crescer profissionalmente esses anos em Bauru. educação e bom humor nos instruíam e auxiliavam em tudo o que precisávamos. Sou eternamente grata por tudo que fizeram por mim. A Cybelle, que teve a maior paciência do mundo para me ajudar no inicio deste trabalho, estava sempre disposta e feliz....

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Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability

Cited by 31 publications

References 38 publications

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

Effects of matrix metalloproteinase enzyms on adhesive bonding

Influence of MMP inhibitors on bond strength of adhesive restorations: systematic review and meta-analysis

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