Experimental dual infection of Leishmania in Phlebotomus langeroni (Diptera: Psychodidae)

Leishmania major: Parasite Interactions Suggesting Sexuality

“…This subject was discredited, until Maazoun et al (1981) reported heterozygous patterns in enzyme electrophoretic variants of three Leishmania species, and their paper was followed by several others describing naturally occurring putative hybrids using biochemical and/or molecular methods (Le Blancq et al 1983, Le Blancq & Peters 1986, Evans et al 1987, Pagès et al 1989, Kelly et al 1991, Cupolillo et al 1992, Belli et al 1994, Piarroux et al 1994, Dujardin et al 1995. However, genetic recombination between species or strains of Leishmania has not yet been demonstrated under laboratory conditions (Gradoni et al 1986, Evans et al 1989, Panton et al 1991, Shehata et al 1991, although Lanotte and Rioux (1990) had recorded by videocinematography fusion of pairs of apposed promastigotes in cultures of two species, and also evidenced the possibility of nuclear fusion by examining these cultures in Giemsa-stained smears.In this paper we describe pairs of apposed promastigotes occurring at relatively high percent + Corresponding author. Fax: +55-21-260.4434 or 290.9339 Received 30 December 1996 Accepted 10 July 1997 in L. (L.) major (P strain) when seeded in a medium described for insect trypanosomatids (Roitman et al 1972), which we had supplemented with fetal calf serum.…”

Zoonotic Cutaneous Leshmaiasis Three Years Fever Hospital Records: With General Discussion

“…The cutaneous form was primarily identified in northern Sinai and was attributed to L. major in man and P. papatasii by isoenzyme typing (Wahba et al, 1990). Infantile visceral leishmaniasis was in Al-Agamy District, West of Alexandria (Faris et al, 1988a) and Dakahlia Governorate (El Mahdy et al, 1993), P. langeroni was the proven vector (Shehata et al, 1991). Faris et al (1988b) identified two ZCL foci in North Sinai Governorate.…”