Experimental determination of rupture pressure and stress of adventitia of human middle cerebral arteries

Piechna, Adam; Lombarski, Leszek; Ciszek, Bogdan; Cieślicki, Krzysztof

doi:10.1177/1747493016685715

Cited by 4 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It probably reflects the ageing of collagen fibres which play the main role in the strength of the vessel wall. It is supported by our investigations on adventitia resistance [10]. However, a small number of specimens limits the possibility of better evaluation of this relationship [8].…”

Section: Discussionsupporting

confidence: 60%

Age dependence of critical pressure in the segments and branches of the circle of Willis

Piechna

Lombarski

Krajewski

et al. 2017

Neurologia i Neurochirurgia Polska

Self Cite

View full text Add to dashboard Cite

Spontaneous intracranial haemorrhage is one of the most dramatic neurological disasters. The source of haemorrhage is linked to the pathology of the arterial wall or is unknown. Because the risk of haemorrhagic stroke increases with age, we tried to investigate the relationship between age and rupture pressure of cerebral arteries. In the presented study, 51 segments of large cerebral arteries (at the level of the circle of Willis and its incoming and outgoing branches) were obtained from 33 cadaver brains aged 12-86 years. The segments were pressurized up to the rupture. The rupture pressure was noted. The highest observed pressure was 4.3atm in specimen aged 24 years. The lowest pressure 1.19atm was observed in specimen aged 80 years. The mean value of rupture pressure of all investigated segments was 2.28atm. Statistical analysis showed a nonlinear exponential decrease of the rupture pressure of the large cerebral arteries with age. The main conclusion from our study is that drop of cerebral arteries strength is observed to the age of 40. Later this lowering is visible but not so evident.

show abstract

Section: Discussionsupporting

confidence: 60%

Age dependence of critical pressure in the segments and branches of the circle of Willis

Piechna

Lombarski

Krajewski

et al. 2017

Neurologia i Neurochirurgia Polska

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because stiff collagen fibres are amenable to slight deformations prior to failure [21], sIA growth is associated with constant processes of collagen degradation and synthesis [3]. In our study, the average rupture pressure of sIAs was lower than the mean rupture pressure of the middle cerebral artery adventitia, which is mainly composed of collagen [17]. Thus, hyalinization may represent regions of collagen degradation and may be responsible for both the growth and rupture of sIAs.…”

Section: Healing and Destructive Processesmentioning

confidence: 54%

Impact of inflammation-related degenerative changes on the wall strength of unruptured intracranial aneurysms: a pilot study

Lombarski¹,

Kunert²,

Tarka³

et al. 2022

View full text Add to dashboard Cite

Introduction: Saccular intracranial aneurysm (sIA) rupture is a serious cerebrovascular event associated with inflammatory destructive processes leading to gradual weakening of the sIA wall. The aim of the present study was to identify the morphological and histological determinants for low wall strength in unruptured sIAs harvested from autopsy subjects. Material and methods: A total of eight single unruptured sIAs were identified and excised with adjacent cerebral arteries during 8 of 184 postmortem examinations. The dome morphology was assessed for each sIA at a constant pressure of 100 mmHg. Then, after 5 preconditioning cycles which assured muscle fibre relaxation, sIA specimens were subjected to gradually increasing intraluminal pressure at a rate of 20 mmHg/s until rupture of the sIA or cerebral artery was achieved. Micro-structural degenerative changes and inflammatory cell infiltration within the sIA wall were quantitatively analysed after pressurization of the sIA specimens. The microscopic analysis of the slides stained with histological methods (HE, Mallory trichrome, Masson trichrome, orcein) and immunohistochemical methods (LCA, CD3, CD68) was performed. Results: The wall of the sIA ruptured in three specimens, while in the other cases, rupture occurred at the arterial wall. The mean maximal dome size was significantly larger in sIAs with low wall strength, that is, in sIAs that ruptured during pressurization, than in sIAs with high wall strength (6.46 mm vs. 2.43 mm, p = 0.034). Moreover, a significantly higher average percentage of wall hyalinization in sIAs that ruptured than in sIAs that did not rupture was observed (30% vs. 0%, p = 0.006). In contrast, the degree of inflammatory cell infiltration did not differ between the wall strength categories. Conclusions: Our results support the observations that larger sIAs may be at a higher risk of rupture. Histological analysis revealed that hyalinization corresponds to the weakened regions of the wall of unruptured sIAs.

show abstract

“…The pressures were ≈60 mm Hg during injection and maximum 260 mm Hg when the contrast solidifies; the values are medically reasonable and >5× lower than the mean pressure needed to cause a rupture of a major intracranial artery. 19,20 To our surprise, in 9 specimens the middle cerebral artery collapsed immediately after the injection, suggesting contrast leakage. However, as there were no visible contrast extravasations, we added some more contrast and poured cold water over the specimen to speed up the solidification of the medium.…”

Section: Methodsmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Mechanism of Spontaneous Intracerebral Hemorrhage Formation: An Anatomical Specimens-Based Study

Rzepliński

Sługocki

Tarka

et al. 2022

Stroke

Self Cite

View full text Add to dashboard Cite

Background: Despite advances in understanding various risk and prognostic factors, spontaneous intracerebral hemorrhage is connected to very high morbidity and mortality, while the therapy is mainly supportive. Understanding of the pathophysiology of initial hematoma expansion is limited due to insufficient clinical data and lack of a suitable animal model. Methods: We injected 40 anatomic specimens of the basal ganglia with contrast medium, scanned them with a micro-computed tomography scanner and analyzed the results of radiological studies, direct and histological examinations. Results: In 9 cases, micro-computed tomography and histological examinations revealed contrast medium extravasations mimicking intracerebral hematomas. The artificial hematomas spread both proximally and distally along the ruptured perforator and its branches in the perivascular spaces and detached the branches from the adjacent neural tissue leading to destruction of the tissue and secondary extravasations. Moreover, some contrast extravasations skipped to the perivascular spaces of unruptured perforators, created further extravasation sites and aggravated the expansion of the artificial hematoma. There was no subarachnoid extension of any artificial hematoma. Conclusions: We postulate that a forming basal ganglia intracerebral hematoma spreads initially in the perivascular space, detaches the branches from the neural tissue and causes secondary bleeding. It can also skip to the perivascular space of a nearby perforator. The proposed mechanism of hematoma initiation and formation explains extent of damage to the neural tissue, variability of growth in time and space, creation of secondary bleeding sites, and limited usefulness of surgical interventions. The model is reproducible, the extent of the artificial hematoma can be easily controlled, the rupture sites of the perforating arteries can be determined, and preparation of the model does not require specialized, expensive equipment apart from the micro-computed tomography scanner.

show abstract

Experimental determination of rupture pressure and stress of adventitia of human middle cerebral arteries

Cited by 4 publications

References 11 publications

Age dependence of critical pressure in the segments and branches of the circle of Willis

Age dependence of critical pressure in the segments and branches of the circle of Willis

Impact of inflammation-related degenerative changes on the wall strength of unruptured intracranial aneurysms: a pilot study

Mechanism of Spontaneous Intracerebral Hemorrhage Formation: An Anatomical Specimens-Based Study

Contact Info

Product

Resources

About