Experimental design of a simple medium for the bioluminescence of Aliivibrio fischeri and mathematical modelling for growth estimation

Abstract

Increasing numbers of studies are using Aliivibrio fischeri (A. fischeri), a marine bioluminescent bacterium as a model, however the culture medium used for its growth are complex and expensive. The objectives of this study were: (1) to evaluate the effect of yeast extract, tryptone, and NaCl to select a simple and inexpensive culture medium suitable for A. fischeri growth and bioluminescence induction; and(2) to compare the performance of mathematical models to predict the growth of A. fischeri. A fractional factorial design was performed to evaluate the effect of yeast extract, tryptone, and sodium chloride on the luminescence of A. fischeri. The result showed that sodium chloride is the most important factor, congruent with its inducer role in bioluminescence. The best medium for bioluminescence induction was selected through an optimization plot, this medium is inexpensive, and generates the same luminescence as commercial formulations. The estimation of A. fischeri growth at OD 600 measurement was statistically analyzed. All evaluated models fitted the data adequately (r 2 > 0.96). The nonlinear models Gompertz, Richards and logistic provided a lower variation and a better fit of the growth estimation (r 2 >0.99),showing that these mathematical models can be used for the accurate growth prediction of A. fischeri.

Harnessing Bioluminescent Bacteria to Develop an Enzymatic-free Enzyme-linked immunosorbent assay for the Detection of Clinically Relevant Biomarkers

“…21 Among the commercially available, naturally bioluminescent bacteria we choseA. fischeri because of its advantages: ability to grow at room temperature (20 °C), the reduced risk of contamination due to the high-salt medium used, the availability of inexpensive culture media, 25 and its For IFE to occur, the absorption spectrum of the absorber must overlap with the emission spectrum of the fluorophore. As a result, when a fixed concentration of fluorophore is titrated into the well with an increasing concentration of absorber, a decrease in fluorescence emission is observed.…”

“…Among the commercially available, naturally bioluminescent bacteria we choseA. fischeri because of its advantages: ability to grow at room temperature (20 °C), the reduced risk of contamination due to the high-salt medium used, the availability of inexpensive culture media, and its stability and activity at room temperature . In fact, although other bacteria share the same biological mechanism and ability to convert chemical energy into bioluminescence, they (such as Photorhabdus luminescens and Vibrio harveyi) require higher temperatures (around 30–35 °C) and, therefore, a temperature-controlled experimental setup .…”