Experimental design for the production of tensio-active agent by Candida lipolytica

Abstract

The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation factors-amounts of refinery residue, glutamic acid and yeast extract. This allowed exclusion of the yeast extract. Full factorials designs were then sequentially used to optimize the levels of the residue and glutamic acid. The surface tension value was finally reduced to 25.29 mN/m. The maximum emulsifier activity using different substrates was within 40 h of cultivation. The surface tension of the cell-free broth containing the biosurfactant remained very stable during exposure to a wide range of pH (2-12), temperatures (0-120 degrees C) and salinity (2-10% NaCl). The combination of an industrial waste and a cheap substrate therefore seems to be very promising for the low-cost production of potent biosurfactant.

Laboratory production and characterization of a new biosurfactant from Candida glabrata UCP1002 cultivated in vegetable fat waste applied to the removal of hydrophobic contaminant

“…As is well known, when there are two carbon sources in the production medium (one lipophilic and the other hydrophilic), higher yields of biosurfactants are obtained by yeasts (Davila et al 1992;Garcia-Ochoa and Casas 1999;Rufino et al 2008;Sarubbo et al 2007). Besides the carbon sources, the nitrogen source also exerts a great influence on biosurfactant production.…”

Formulação Do Biossurfactante Produzido Por Candida Lipolytica Para Aplicação Na Remoção De Poluentes Ambientais Gerados Pela Indústria De Petróleo Contidos Em Água Do Mar

“…O biossurfactante foi produzido em meio mineral contendo resíduos industriais como substratos (Rufino et al, 2008).…”

Replacing Synthetic with Microbial Surfactants as Collectors in the Treatment of Aqueous Effluent Produced by Acid Mine Drainage, Using the Dissolved Air Flotation Technique

“…The medium was based on distilled water supplemented with ground-nut oil refinery residue plus corn steep liquor as substrates. The biosurfactant produced showed high surface tension reducing activity (26 mN/m) and a small CMC value (0.08%), while Rufino et al [30] produced an anionic biosurfactant from C. lipolytica grown in vegetable oil refinery residue and glutamic acid which reduced the surface tension to 26 mN/m at 0.03% concentration. More recently, Luna et al [31] described the use of statistical experimental designs and response surface methodology to optimize the concentrations of soybean oil refinery residue and corn steep liquor for biosurfactant production by C. sphaerica.…”