Experimental design and response surface modeling applied for the optimisation of pectin hydrolysis by enzymes from A. niger CECT 2088

Rodríguez‐Nogales, José Manuel; Ortega, Natividad; Pérez-Mateos, M.; Busto, María D.

doi:10.1016/j.foodchem.2006.01.055

Cited by 28 publications

(10 citation statements)

References 18 publications

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“…Single factor analysis are laborious and time-consuming methods and often does not guarantee the determination of the optimal conditions (Box et al, 2005;Wang et al, 2013). On the other hand, carrying out experiments using every possible combination of the test variables is impractical due to the associated large number of experiments required (Rodríguez-Nogales et al, 2007). One viable strategy consists on selecting, firstly, the variables playing a significant role in the extraction yield, and then applying the statistical multi-response optimization using a response surface methodology (RSM).…”

Section: Introductionmentioning

confidence: 99%

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

Heleno

Prieto

Barros

et al. 2016

Food and Bioproducts Processing

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This work intends to valorise by-products of the industrial processing of mushrooms to obtain ergosterol as a value-added compound. Agaricus bisporus L. is the world's most consumed mushroom and one of the richest sources of ergosterol. Microwave-assisted extraction was used to replace conventional techniques that are time-consuming and need large amounts of solvent. Time (3-20 min), temperature (60-210 ºC) and solid-liquid ratio (1-20 g/L) werefound the relevant variables to analyze the extraction process. To maximize the ergosterol extraction yield, response surface methodology was used to optimize the process. The global optimal extraction conditions were determined and comprise: 19.4±2.9 min, 132.8±12.4 ºC and 1.6±0.5 g/L, yielding 556.1±26.2 mg of ergosterol per 100 g of mushroom by-products.In the MAE optimal conditions, it was possible to obtain ergosterol in a similar value to the one obtained in other works when using the Soxhlet extraction method with a significant decrease in the time of extraction. The results show the potential of using the by-products of an agroindustry, mushrooms processing industry, as productive sources of ergosterol.

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Section: Introductionmentioning

confidence: 99%

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

Heleno

Prieto

Barros

et al. 2016

Food and Bioproducts Processing

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“…As a result of the OFAT experiments the enzyme concentration, substrate concentration, temperature, pH and reaction time were selected to optimize the enzymatic hydrolysis process [27]. Tab.…”

Section: Response Surface Modeling For Enzymatic Hydrolysismentioning

confidence: 99%

Enzymatic Hydrolysis of Corn Stover after Pretreatment with Dilute Sulfuric Acid

et al. 2007

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Although many previous studies have been carried on the enzymatic hydrolysis of corn stover after pretreatment with dilute sulfuric acid, this paper emphasizes the use of different conditions to attain the highest yields of two sugars, xylose and glucose, from both stages. The pretreatment was performed at a range of sulfuric acid concentrations of 2, 4 and 6 % at 80, 100 and 120°C. Up to 77 % xylose yield was obtained while the glucose yield was only 8.4 %. The corresponding solid phase was hydrolyzed by cellulase and the influences of five factors and their interactions on enzyme hydrolysis were evaluated by response surface methodology based on one-factor-at-a-time experiments. The optimal levels for each variable to obtain the highest reducing sugar yield were as follows: enzyme concentration of 22 FPU/g substrate, substrate concentration of 77 g/L, temperature of 49°C, pH 4.8 and reaction time of 38 h. A reducing sugar yield of 42.11 g/100 g substrate was achieved, which was consistent with the predicted value of 42.13 g/ 100 g substrate. Compared with the one-factor-at-a-time experiments, there was a 9.4 % increase in reducing sugar yield when the enzyme concentration was decreased to 3 FPU/g substrate, the substrate concentration increased to 17 g/L and the reaction time dropped to 22 h. The total sugar released from the two stages was 62.81 g/100 g substrate.

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“…Optimization of reaction parameters for conversion of acrylonitrile to acrylamide by R. rhodochrous PA-34 showed rallied effect on acrylamide yield [20]. Successful experimental design and RSM have been applied for the reaction optimization of pectin hydrolysis and for the synthesis of sorbitan methacrylate [21,22]. To the best of our knowledge, there are few reports that describe the use of RSM for the optimization of reaction conditions in the production of nicotinamide by NHase.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Crucial Reaction Conditions for the Production of Nicotinamide by Nitrile Hydratase Using Response Surface Methodology

Kamble

Banerjee

2008

Appl Biochem Biotechnol

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The reaction conditions for the nicotinamide production by Rhodococcus erythropolis MTCC 1526 have been optimized by statistical experimental design. Application of this approach in the bioprocess can result in rallied product yield, reduced development time, and process variability. In this investigation, response surface methodology and central composite design were employed to predict the levels of variables such as reaction pH (6.5, 7, and 7.5), temperature (15, 20, and 25 degrees C), cell concentration (190, 200, and 210 mg/ml), and substrate concentration (18, 20, and 22 mM) on the production of nicotinamide. A total of 22 experiments were carried out in shake flasks, and a three-dimensional response surface was generated to determine the effect of crucial reaction parameters for the maximum conversion of 3-cynopyridine to nicotinamide. Using this methodology, the optimal values for the reaction conditions were reaction pH 6.85, temperature of 24.8 degrees C, cell concentration of 190.98 mg/ml, and substrate concentration of 21.98 mM. This statistical approach led to the increase of conversion of 3-cynopyridine (93%) as compared to the conversion obtained by one-factor-at-a-time approach (84%).

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Experimental design and response surface modeling applied for the optimisation of pectin hydrolysis by enzymes from A. niger CECT 2088

Cited by 28 publications

References 18 publications

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

Enzymatic Hydrolysis of Corn Stover after Pretreatment with Dilute Sulfuric Acid

Optimization of Crucial Reaction Conditions for the Production of Nicotinamide by Nitrile Hydratase Using Response Surface Methodology

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