Experimental comparison of two methods to study barley responses to partial submergence

Miricescu, Alexandra; Byrne, Tomás; Doorly, Catherine M.; Ng, Carl; Barth, Susanne; Graciet, Emmanuelle

doi:10.1186/s13007-021-00742-5

Cited by 16 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Highly controlled conditions and growth media allow for the isolation of anoxia stress by removing compounding factors found in the field or pots. Laboratory-based methods such as hydroponics, agar, and starch have been used to simulate the hypoxic conditions of waterlogging ( Hunt et al , 2002 ; Bertholdsson, 2013 ; Miricescu et al , 2021 ). The use of such methods with a tighter control over the growth media, i.e.…”

Section: What Are the Advantages And Challenges When Phenotyping For ...mentioning

confidence: 99%

Phenotyping for waterlogging tolerance in crops: current trends and future prospects

Langan

Bernád

Walsh

et al. 2022

Journal of Experimental Botany

View full text Add to dashboard Cite

Yield losses to waterlogging are expected to become an increasingly costly and frequent issue in some regions of the world. Despite the extensive work that has been carried out examining the molecular and physiological responses to waterlogging, phenotyping for waterlogging tolerance has proven difficult. This difficulty is largely due to the high variability of waterlogging conditions such as duration, temperature, soil type, and growth stage of the crop. In this review, we aim to highlight efforts utilising phenotyping to assess and improve waterlogging tolerance in temperate crop species. We start by outlining the experimental methods that have been utilised to impose waterlogging stress, ranging from highly controlled conditions of hydroponic systems to large-scale screenings in the field. We also describe the phenotyping traits used to assess tolerance ranging from survival rates and visual scoring to precise photosynthetic measurements. Finally, we present an overview of the challenges faced in attempting to improve waterlogging tolerance, the trade-offs associated with phenotyping in controlled conditions, limitations of classic phenotyping methods and future trends using plant-imaging methods. If effectively utilised to increase crop resilience to changing climates, crop phenotyping has a major role to play in global food security.

show abstract

Section: What Are the Advantages And Challenges When Phenotyping For ...mentioning

confidence: 99%

Phenotyping for waterlogging tolerance in crops: current trends and future prospects

Langan

Bernád

Walsh

et al. 2022

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…As one of the severe abiotic stress factors, waterlogging stress negatively affected the development and growth of crops (Yu et al 2020, Miricescu et al 2021. 6-BA is a cytokinin and an artificial synthetic plant growth regulator, which can help crops overcome the negative effects of abiotic stresses, including waterlogging (Ghaleh et al 2020, Hu et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Mitigative effect of 6-benzyladenine on photosynthetic capacity and leaf ultrastructure of maize seedlings under waterlogging stress

WANG¹,

WANG²,

WANG³

et al. 2022

Photosynt.

View full text Add to dashboard Cite

6-Benzyladenine (6-BA) is an artificial synthetic cytokinin, which plays an important role in regulating plant responses to abiotic stress. This study aimed to investigate the mitigative effect of exogenous 6-BA on photosynthetic capacities and leaf ultrastructure under waterlogging stress using two waxy corn inbred lines. The results showed that waterlogging stress disrupted the photosynthesis of waxy corn seedlings. However, exogenous 6-BA alleviated the inhibition caused by waterlogging stress. Under the waterlogging conditions, 6-BA treatment of plants helped preserve the structural integrity of the chloroplasts and retain higher contents of photosynthetic pigments. It also increased the photosynthetic capacity and promoted the openness of stomatal pores. Moreover, exogenous 6-BA promoted photosynthetic activities and the accumulation of carbohydrates. The results revealed that the detrimental effects of waterlogging stress on maize seedlings can be alleviated by 6-BA via modulating photosynthetic activities. Highlights• Two waxy corn inbred lines were used in this study • Exogenous 6-BA improved photosynthetic capacity and leaf ultrastructure • Exogenous 6-BA alleviated the waterlogging-induced damages of waxy corn seedlings

show abstract

“…Each independent replicate was obtained by pooling the root systems from 3 plants prior to grinding for RNA extraction. Note: expression values for Infinity in (B) and (C) were published in (Miricescu et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Waterlogging (as well as flooding) is an important source of crop losses (de San Celedonio et al ., 2014; Kaur et al ., 2020), however, not all crop species are equally affected. Barley has been identified as a particularly sensitive crop with up to 20-25% crop losses due to waterlogging (Byrne et al ., 2021; de San Celedonio et al ., 2016; de San Celedonio et al ., 2014; Liu et al ., 2020; Miricescu et al ., 2021; Setter and Waters, 2003). Losses have been shown to be more severe if waterlogging occurs at the heading stage (de San Celedonio et al ., 2016; de San Celedonio et al ., 2014; Liu et al ., 2020; Setter and Waters, 2003), but also at early developmental stages (de San Celedonio et al ., 2016; de San Celedonio et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Here, to identify barley varieties that are waterlogging sensitive or tolerant, we used varieties from the Association Genetics Of UK Elite Barley (AGOUEB) population, which consists of barley varieties that have been commercialized on the UK National and Recommended List trials between 1988 and 2006 (Thomas et al ., 2014). Next, we characterized selected varieties, using a previously established controlled-condition waterlogging protocol (Miricescu et al ., 2021). We then dissected the transcriptomic response of two 2-row and two 6-row winter barley varieties that were moderately sensitive and tolerant to waterlogging in order to identify genes whose expression may be associated with these traits.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response

Miricescu

Brazel

Beegan

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Waterlogging leads to major crop losses globally, particularly for waterlogging sensitive crops such as barley. Waterlogging reduces oxygen availability and results in additional stresses, leading to the activation of hypoxia and stress response pathways that promote plant survival. Although certain barley varieties have been shown to be more tolerant to waterlogging than others, the molecular mechanisms underlying this trait are mostly unknown. Here, we surveyed 21 barley varieties for the transcriptional activation of conserved hypoxia-response genes under waterlogging. We selected five varieties that showed differential induction of core hypoxia-response genes during waterlogging treatment and further characterized their phenotypic response to waterlogging stress, with the aim of categorizing them as waterlogging sensitive or tolerant varieties. RNA-sequencing to evaluate the transcriptional responses of these selected varieties to waterlogging led to the identification of a set of 98 core waterlogging-response genes in barley. We further identified groups of genes whose expression correlated with the varietal differences in waterlogging tolerance. These datasets identify potential candidates for molecular markers and/or gene targeting to improve waterlogging tolerance in barley.HighlightThis study identifies 5 barley varieties with different tolerance to waterlogging. We provide the first RNA-seq datasets identifying both conserved and unique transcriptional responses in both waterlogging tolerant and sensitive barley varieties.

show abstract

Experimental comparison of two methods to study barley responses to partial submergence

Cited by 16 publications

References 36 publications

Phenotyping for waterlogging tolerance in crops: current trends and future prospects

Phenotyping for waterlogging tolerance in crops: current trends and future prospects

Mitigative effect of 6-benzyladenine on photosynthetic capacity and leaf ultrastructure of maize seedlings under waterlogging stress

Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response

Contact Info

Product

Resources

About