Experimental botany in 2017

Raines, Christine A.; Traynor, Mary; Ingram, Jonathan

doi:10.1093/jxb/erw504

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…Volatile metabolites were trapped by means of headspace solid phase micro-extraction (HS-SPME) and analyzed by gas chromatography coupled to mass spectrometry (GC/MS) ( 65 ). Briefly, frozen tuber potato sections were ground in a cryogenic mill.…”

Section: Methodsmentioning

confidence: 99%

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Gómez

Morote²,

Frusciante³

et al. 2022

Front. Nutr.

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Carotenoids are C40 isoprenoids with well-established roles in photosynthesis, pollination, photoprotection, and hormone biosynthesis. The enzymatic or ROS-induced cleavage of carotenoids generates a group of compounds named apocarotenoids, with an increasing interest by virtue of their metabolic, physiological, and ecological activities. Both classes are used industrially in a variety of fields as colorants, supplements, and bio-actives. Crocins and picrocrocin, two saffron apocarotenoids, are examples of high-value pigments utilized in the food, feed, and pharmaceutical industries. In this study, a unique construct was achieved, namely O6, which contains CsCCD2L, UGT74AD1, and UGT709G1 genes responsible for the biosynthesis of saffron apocarotenoids driven by a patatin promoter for the generation of potato tubers producing crocins and picrocrocin. Different tuber potatoes accumulated crocins and picrocrocin ranging from 19.41–360 to 105–800 μg/g DW, respectively, with crocetin, crocin 1 [(crocetin-(β-D-glucosyl)-ester)] and crocin 2 [(crocetin)-(β-D-glucosyl)-(β-D-glucosyl)-ester)] being the main compounds detected. The pattern of carotenoids and apocarotenoids were distinct between wild type and transgenic tubers and were related to changes in the expression of the pathway genes, especially from PSY2, CCD1, and CCD4. In addition, the engineered tubers showed higher antioxidant capacity, up to almost 4-fold more than the wild type, which is a promising sign for the potential health advantages of these lines. In order to better investigate these aspects, different cooking methods were applied, and each process displayed a significant impact on the retention of apocarotenoids. More in detail, the in vitro bioaccessibility of these metabolites was found to be higher in boiled potatoes (97.23%) compared to raw, baked, and fried ones (80.97, 78.96, and 76.18%, respectively). Overall, this work shows that potatoes can be engineered to accumulate saffron apocarotenoids that, when consumed, can potentially offer better health benefits. Moreover, the high bioaccessibility of these compounds revealed that potato is an excellent way to deliver crocins and picrocrocin, while also helping to improve its nutritional value.

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Section: Methodsmentioning

confidence: 99%

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Gómez

Morote²,

Frusciante³

et al. 2022

Front. Nutr.

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“…Volatile compounds were captured by HS-SPME and analyzed by GC–MS. Analyses of the volatile compounds were performed similarly to a previously described process [ 55 ]. Roughly, 500 mg of the resulting powder from tomato fruits was introduced into a 15 mL glass vial and incubated at 37°C for 10 min in a water bath.…”

Section: Methodsmentioning

confidence: 99%

Engineering high levels of saffron apocarotenoids in tomato

Ahrazem

Diretto

Rambla

et al. 2022

Horticulture Research

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Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron and used commercially in the food and pharmaceutical industries. These apocarotenoids are derived from the oxidative cleavage of zeaxanthin by specific carotenoid cleavage dioxygenases. The pathway for crocins and picrocrocin biosynthesis was introduced into tomato using fruit specific and constitutive promoters and resulted in 14.48 mg/g of crocins and 2.92 mg/g of picrocrocin in the tomato DW, without compromising plant growth. The strategy involved expression of CsCCD2L to produce crocetin dialdehyde and 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene, and of glycosyltransferases UGT709G1 and CsUGT2 for picrocrocin and crocins production, respectively. Metabolic analyses of the engineered fruits revealed picrocrocin and crocetin-(β-D-gentiobiosyl)-(β-D-glucosyl)-ester, as the predominant crocin molecule, as well as safranal, at the expense of the usual tomato carotenoids. The results showed the highest crocins content ever obtained by metabolic engineering in heterologous systems. In addition, the engineered tomatoes showed higher antioxidant capacity and were able to protect against neurological disorders in a Caenorhabditis elegans model of Alzheimer's disease model. Therefore, these new developed tomatoes could be exploited as a new platform to produce economically competitive saffron apocarotenoids with health-promoting properties.

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Experimental botany in 2017

Cited by 2 publications

References 33 publications

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Engineering high levels of saffron apocarotenoids in tomato

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