Experimental Approaches to Study Endothelial Responses to Shear Stress

Bowden, Neil; Bryan, Matthew T.; Duckles, Hayley; Feng, Shuang; Hsiao, Sarah; Kim, Hyejeong Rosemary; Mahmoud, Marwa; Moers, Britta; Serbanovic-Canic, Jovana; Xanthis, Ioannis; Ridger, Victoria; Evans, Paul C.

doi:10.1089/ars.2015.6553

Cited by 13 publications

(8 citation statements)

References 103 publications

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“…Including shear stress may improve future endothelial cell drug discovery or toxicity screening and increase clinical trial success rates. A range of methods have been developed to apply shear stress to endothelial cell cultures in vitro , including parallel-plate flow chambers, cone-and-plate viscometers, orbital shakers and microfluidics [12], each having advantages and disadvantages. An important drawback with many is the challenge in scaling up for high-throughput screening.…”

Section: Introductionmentioning

confidence: 99%

Using Yoda-1 to mimic laminar flow in vitro: A tool to simplify drug testing

Davies

Lopresto

Apta

et al. 2019

Biochemical Pharmacology

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Section: Introductionmentioning

confidence: 99%

Using Yoda-1 to mimic laminar flow in vitro: A tool to simplify drug testing

Davies

Lopresto

Apta

et al. 2019

Biochemical Pharmacology

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“…This has inspired a drive to produce microbots capable of entering the body to perform useful therapeutic functions, such as microdrilling 1 or directing drug delivery to locally increase concentration in target sites while limiting off-target dosage elsewhere, therefore reducing side effects. 2 Additionally, the potential of microbots for cell stimulation 3 and transportation 4 have made them desirable for chip-based assays and microfluidic applications. Key to the achieving this goal is the ability to create directed movement through a fluid.…”

Section: Introductionmentioning

confidence: 99%

Emergent propagation modes of ferromagnetic swimmers in constrained geometries

Bryan

Shelley

Parish

et al. 2017

Journal of Applied Physics

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Magnetic microswimmers, composed of hard and soft ferromagnets connected by an elastic spring, are modelled under low Reynolds number conditions in the presence of geometrical boundaries. Approaching a surface, the magneto-elastic swimmer's velocity increases and its trajectory bends parallel to the surface contour. Further confinement to form a planar channel generates new propagation modes as the channel width narrows, altering the magneto-elastic swimmer's speed, orientation and direction of travel. Our results demonstrate that constricted geometric environments, such as occur in microfluidic channels or blood vessels, may influence the functionality of magnetoelastic microswimmers for applications such as drug delivery.2

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“…This is attributable to the substantial impact of mechanotransduction on the homeostasis of vascular endothelial cells [84,87]. In line with the importance of shear stress for the pathophysiology of this cell type, a recent review from Bowden and colleagues described in detail specific methods for the integration of the biomechanical stimulation in the experimental layout [89]. Endothelial cells are dependent on shear stress for the handling of reactive oxygen species (ROS) and for the suppression of pro-inflammatory cytokines [90].…”

Section: Endothelial Cellsmentioning

confidence: 99%

Integrating Biophysics in Toxicology

Favero

Kraegeloh

2020

Cells

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Integration of biophysical stimulation in test systems is established in diverse branches of biomedical sciences including toxicology. This is largely motivated by the need to create novel experimental setups capable of reproducing more closely in vivo physiological conditions. Indeed, we face the need to increase predictive power and experimental output, albeit reducing the use of animals in toxicity testing. In vivo, mechanical stimulation is essential for cellular homeostasis. In vitro, diverse strategies can be used to model this crucial component. The compliance of the extracellular matrix can be tuned by modifying the stiffness or through the deformation of substrates hosting the cells via static or dynamic strain. Moreover, cells can be cultivated under shear stress deriving from the movement of the extracellular fluids. In turn, introduction of physical cues in the cell culture environment modulates differentiation, functional properties, and metabolic competence, thus influencing cellular capability to cope with toxic insults. This review summarizes the state of the art of integration of biophysical stimuli in model systems for toxicity testing, discusses future challenges, and provides perspectives for the further advancement of in vitro cytotoxicity studies.

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Experimental Approaches to Study Endothelial Responses to Shear Stress

Abstract: We conclude that combining in vitro and in vivo approaches can provide a detailed mechanistic view of vascular responses to force and that high-throughput systems are required for unbiased assessment of the function of shear-induced molecules. Antioxid. Redox Signal. 25, 389-400.

Cited by 13 publications

References 103 publications

Using Yoda-1 to mimic laminar flow in vitro: A tool to simplify drug testing

Using Yoda-1 to mimic laminar flow in vitro: A tool to simplify drug testing

Emergent propagation modes of ferromagnetic swimmers in constrained geometries

Integrating Biophysics in Toxicology

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