Experimental and visual research on the microbial induced carbonate precipitation by Pseudomonas aeruginosa

Abstract

Microbial induced carbonate precipitation (MICP) is a common occurrence of geochemistry influences in many fields, such as biological, geographical, and engineering systems. However, the processes that control interactions between carbonate biomineralization and biofilm properties are poorly understood. We develop a method for real time, in situ and nondestructive imaging with confocal scanning microscopy. This method provides a possible way to observe biomineralization process and the morphology of biomineralized deposits within biofilms. We use this method to show calcite biominerals produced by Pseudomonas aeruginosa biofilms which extremely change biofilm structures. The distribution of calcite precipitation produced in situ biomineralization is highly heterogeneous in biofilms and also to occur primarily on the bottom of biofilms. It is distinct from those usual expectations that mineral started to precipitate from surface of biofilm. Our results reveal that biomineralization plays a comprehensive regulation function on biofilm architecture and properties.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0358-5) contains supplementary material, which is available to authorized users.

Environmental and Genetic Determinants of Biofilm Formation in Paracoccus denitrificans

“…Bacteria are well known to mineralize calcium, and denitrification is one of the metabolic processes associated with calcium carbonate formation ( 58 ). Calcium carbonate has been detected in bacterial biofilms, for example, in the case of Pseudomonas aeruginosa ( 59 ); thus, we speculate that BapA might function to nucleate the formation of a calcium mineral that is required for cell-cell and cell-surface interactions. An alternative possibility is that binding of calcium ions to BapA alters the structure of the protein in a way that is required for it to function as an adhesin.…”

Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration

“…The removal of organic matter in the hybrid MBBR-MBR system subjected to variable salinity conditions was very good during the whole steady-state operation period with 99.51% removal of BOD 5 and 88.64% removal of COD for mean influent values of 293. 33 [20]. For the constant salinity scenario, the removal rates of BOD 5 and COD were 98.09% and 88.55% for influent concentrations of 408.33 ± 46.18 mg-BOD 5 L −1 and 654.07 ± 154.57 mg-COD L −1 , respectively.…”

“…For the constant salinity condition, the identified phylotypes with >1% relative abundance reported for calcite precipitation were Nocardioides (1.163% to 1.694%) [31], Brevibacterium (0.604% to 2.324%) [32], and Pseudomonas (0.671% to 2.284%) [33]. For the case of struvite precipitation, the identified phylotypes were Corynebacterium (0.492% to 2.374%) [34], Brevibacterium (0.604% to 2.324%) [35], and Ochrobactrum (0.671% to 2.284%) [15].…”

“…Furthermore, we must highlight that, in conditions of variable salinity, B. licheniformis was the only cultivable microorganism with the ability to form struvite minerals. The other Bacillus representative, Bacillus pumilus, has been reported for having a strong capacity for struvite crystallization from wastewater [33]. The relative abundance of Bacillus genus in the constant salinity scenario was from 0.015% to 0.029% while, for the case of the variable salinity scenario, ranged from 0.009% to 0.014%.…”

Plasticity characteristics of lateritic soil treated with Sporosarcina pasteurii in microbial-induced calcite precipitation application

“…An important requirement for effective MICP process is the presence of microorganism which serves as nucleation site. The microorganisms interact with each other and solid surface to form what is known as biomass or biofilm, which is a combination of buildup of single or complex organism cells and extracellular polymeric substances (EPS) [8,39]. Proto et al [65] reported that the high specific surface area of soils facilitates the formation of biofilms that significantly have the potential to influence the engineering behavior of soil.…”