Experimental and theoretical study of novel aminobenzamide–aminonaphthalimide fluorescent dyads with a FRET mechanism

Abstract

The aminobenzamide–aminonaphthalimide fluorescent dyads allow the determination of Cu2+ and Hg2+ metal ion concentration from Förster Resonant Energy Transfer measurements.

Protein‐based metal bio‐cleaner for detoxification of wastewater

“…Similarly, some variants are sensitive to divalent metal ions and depend on charge association, ionic radius and structural geometries of surrounding amino acids. In GFP, there are 11 histidines; among them, position 148 is close to the chromophore and is responsible for charge stabilization and chromophore relaxation 27,28 . The copper ion interaction profile tends to involve coordinating interaction with GFP, especially the NH 2 group of histidine and the carboxyl group of aspartic and glutamic acids in the process of charge neutralization 9 .…”

“…The cell pellets were washed twice with 0.9% (w/v) NaCl solution and then resuspended in M9 minimal medium supplemented with 19 amino acids (40 mg L −1 ) except tyrosine. The cells were grown with a minimum amount of tyrosine (0.03 mmol L −1 ) for 4–5 h, then OD 600 was measured every 30 min to confirm the tyrosine depletion in the media 9,27‐29 . After growth arrest, the culture was split into two: one was supplemented with l ‐tyrosine (40 mg L −1 ) as a positive control; the other was a test sample with 3‐aminotyrosine (1 mmol L −1 ) and IPTG (1 mmol L −1 ) to induce protein expression.…”

“…The cells were grown with a minimum amount of tyrosine (0.03 mmol L −1 ) for 4-5 h, then OD 600 was measured every 30 min to confirm the tyrosine depletion in the media. 9,[27][28][29] After growth arrest, the culture was split into two: one was supplemented with L-tyrosine (40 mg L −1 ) as a positive control; the other was a test sample with 3-aminotyrosine (1 mmol L −1 ) and IPTG (1 mmol L −1 ) to induce protein expression. These cultures were allowed to grow overnight, and then the harvested cells were stored at −70 °C for further use.…”

“…Hence, this trend of fluorescence quenching can be used as a probe to sense divalent metal ions, especially Cu 2+ in solution. 9,11,26,27 The most important key feature of fluorescent proteins in developing biocompatible probes for sensing metal ions is the change in fluorescent property of the proteins when they interact with metal ions. Mostly, the mutation occurs in the ⊎-7 and ⊎-10 region of GFP which interacts with the metal ions depending on charge association, ionic radius and structural geometry of surrounding amino acids.…”

A Highly Water-Soluble and Solid State Emissive 1,8-Naphthalimide as a Fluorescent PET Probe for Determination of pHs, Acid/Base Vapors, and Water Content in Organic Solvents

“…In the past years, the development of fluorescent sensors and fluorescent sensing materials has been a topic of research in chemistry [1][2][3][4][5]. The fluorescence signaling output attracts due to several advantages such as cheap equipment with high sensitivity, immediate response, harmless and non-invasiveness suitable for real-time bioimaging and diagnostic medicine [6][7][8][9][10][11].…”