Experimental and seasonal exposure to birch pollen in allergic rhinitis and allergic asthma with regard to the inflammatory response

Kämpe, Mary; Janson, Christer; Stålenheim, Gunnemar; Stolt, Ingrid; Carlson, Marie

doi:10.1111/j.1752-699x.2009.00140.x

Cited by 9 publications

(14 citation statements)

References 33 publications

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“…Exposure to natural birch pollen is well established as a way of inducing airway inflammation and can be used to study the inflammatory response of the epithelium in the airways (Tilles & Bardana, ; Kampe et al ., ). This was evident also in the present study according to the FENO results.…”

Section: Discussionmentioning

confidence: 98%

Exhaled particles as markers of small airway inflammation in subjects with asthma

Larsson

Lärstad

Bake

et al. 2015

Clin Physio Funct Imaging

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Exhaled breath contains suspended particles of respiratory tract lining fluid from the small airways. The particles are formed when closed airways open during inhalation. We have developed a method called Particles in Exhaled air (PExA ) to measure and sample these particles in the exhaled aerosol. Here, we use the PExA method to study the effects of birch pollen exposure on the small airways of individuals with asthma and birch pollen allergy. We hypothesized that birch pollen-induced inflammation could change the concentrations of surfactant protein A and albumin in the respiratory tract lining fluid of the small airways and influence the amount of exhaled particles. The amount of exhaled particles was reduced after birch pollen exposure in subjects with asthma and birch pollen allergy, but no significant effect on the concentrations of surfactant protein A and albumin in exhaled particles was found. The reduction in the number of exhaled particles may be due to inflammation in the small airways, which would reduce their diameter and potentially reduce the number of small airways that open and close during inhalation and exhalation.

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Section: Discussionmentioning

confidence: 98%

Exhaled particles as markers of small airway inflammation in subjects with asthma

Larsson

Lärstad

Bake

et al. 2015

Clin Physio Funct Imaging

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“…Despite these differences, both patient groups showed a similar degree of eosinophil inflammation both locally and systemically during pollen season as well as after both nasal and bronchial challenge [23,24]. Our hypothesis was therefore that differences in degranulation patterns contribute to the outcome of different clinical manifestations between the allergic groups.…”

Section: Discussionmentioning

confidence: 99%

Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge

et al. 2011

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BackgroundPatients with allergic rhinitis and allergic asthma demonstrate comparable local and systemic eosinophil inflammation, and yet they present with different clinical pictures. Less is even known about the contribution of neutrophil inflammation in allergic diseases. The aim of the study was to examine the propensity and selectivity of granule release from primed systemic eosinophils and neutrophils in allergic rhinitis and allergic asthma after seasonal and experimental allergen exposure. We hypothesize that the dissimilar clinical manifestations are due to diverse eosinophil and neutrophil degranulation.MethodsNine birch pollen allergic patients with rhinitis, eight with asthma and four controls were studied during pollen season and after nasal and bronchial allergen challenge. Eosinophils and neutrophils were incubated in vitro with assay buffer and opsonized Sephadex particles for spontaneous and C3b-induced granule protein release. The released amount of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) was measured by specific radioimmunoassay.ResultsC3b-induced degranulation resulted in increased release of ECP and MPO from primed blood eosinophils and neutrophils in both allergic rhinitis and allergic asthma during pollen season and after both nasal and bronchial challenge (p-values 0.008 to 0.043). After bronchial challenge, the ECP release was significantly higher in the rhinitic group compared to the asthmatic group [19.8 vs. 13.2%, (p = 0.010)]. The propensity for EPO release was weak in all challenge models but followed the same pattern in both allergic groups.ConclusionsSystemically activated eosinophils and neutrophils have similar patterns of degranulation after allergen exposure in allergic rhinitis and allergic asthma. The released amount of ECP, EPO and MPO was similar in all allergen challenge models in both allergic groups. Our results indicate that other mechanisms than the magnitude of eosinophil and neutrophil inflammation or the degranulation pattern of the inflammatory cells determines whether or not an allergic patient develops asthma.

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“…However, patients with allergic asthma were more responsive as measured by FEV 1 decline to inhalation of hypertonic 4.5% saline solution at baseline, had a greater decrease in both morning and evening PEFR during pollen season and also had a greater responsiveness expressed as allergen PD 20 for birch after bronchial challenge [21,22] ( Table 1).…”

Section: Clinical Characteristicsmentioning

confidence: 93%

“…When pollen counts reached 4,000 grains/m 3 , the subjects were told to start recording their morning and evening peak expiratory flow rate (PEFR) in a diary until the season visit. The design of the present study has been described in detail in previous reports [21,22].…”

Section: Methodsmentioning

confidence: 99%

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

et al. 2011

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The PI3K pathway plays a major role in many vital cell processes. Our primary aim was to investigate signalling through PI3K for in vitro degranulation from allergen-primed eosinophils and neutrophils in allergic rhinitis and allergic asthma after seasonal and experimental allergen challenge. Nine patients with allergic rhinitis, eight with allergic asthma and four controls were studied during birch pollen season and after nasal and bronchial allergen challenge. Primed blood eosinophils and neutrophils were stimulated for in vitro degranulation with C3b-coated Sephadex particles, after prior incubation with Wortmannin, a PI3K inhibitor. The released amounts of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) were measured by radioimmunoassay. Wortmannin (10(-6) to 10(-9) M) inhibited ECP, EPO and MPO release in a dose-dependent manner in allergic rhinitis and allergic asthma in all three allergen challenge models. Inhibition of ECP release tended to be lower in the asthmatics in all allergen challenge models, statistically significant compared to the controls during season for 10(-8) M Wortmannin (p=0.01). A clear propensity towards less inhibition in the rhinitic patients was seen after nasal and bronchial challenge compared to seasonal exposure, significant for ECP (10(-8) M Wortmannin; p=0.034 and 0.002, respectively). Signalling through PI3K is clearly involved in ECP, EPO and MPO release in allergic rhinitis and allergic asthma irrespective of allergen challenge model. Allergic asthma demonstrated less inhibition of ECP release via PI3K during pollen season, indicating that other pathways play a greater role in eosinophil degranulation in allergic asthma than allergic rhinitis.

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Experimental and seasonal exposure to birch pollen in allergic rhinitis and allergic asthma with regard to the inflammatory response

Abstract: Bronchial allergen challenge with inhalation of birch pollen gives a similar inflammatory response in the airway but less systemic inflammation than seasonal exposure in birch pollen allergic patients with asthma and rhinitis.

Cited by 9 publications

References 33 publications

Exhaled particles as markers of small airway inflammation in subjects with asthma

Exhaled particles as markers of small airway inflammation in subjects with asthma

Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

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