Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 85 19a. NAME OF RESPONSIBLE PERSON USAMRMC a. REPORT b. ABSTRACT c. THIS PAGE 19b. TELEPHONE NUMBER (include area code) 1.1.3. Determine if alterations in vehicle hull design, particularly those that reduce both maximal G-force and JERK, reduce histologic, neurochemical, or behavioral indices of brain injury.2.0 Technical Requirements:
Quantify physiologic, neurochemical, and neuro-histopathologic TBI outcomes after exposure of rats to underbody blast-induced hyperacceleration. Compare these outcomes to direct measurements of acceleration (G-force) and acceleration rate (JERK) to determine minimal and maximal survivable loads associated with TBI, to establish dose-dependence relationships, and to identify the neurobiologic alterations most closely linked with the pathophysiology of this form of TBI. (Aligned with Objective 1)2.1.1. Expose anesthetized test animals (rats) to defined degrees of blast-associated acceleration forces while secured on a metal structure that simulates a closed armored vehicle.Approximately 38 rats have been subjected to underbody blasts resulting in peak vertical accelerations ranging from 10 Gs to 2000 Gs. Accelerometer measurements confirmed target G forces for all but 3 blasts (6 rats). All rats survived these procedures and did not exhibit any external injuries. Two rats that were exposed to the 2000 G underbody blast exhibited evidence of minor lung hemorrhages when perfusion fixed at 7 days post-blast.2.1.2 Utilize a subset of animals for MRI and MRS measurements performed at one day prior to blast exposure (baseline) and again at several times post-blast.
Eight of the rats exposed to 2000 G underbody blast were used for MRI/MRS measurements performed at baseline (one day prior to blast), and 2 hr, 24 hr and 7 days post-blast. Representative preliminary results from diffusion tensor imaging (DTI) and magnetic resonance spectroscopy (MRS) are shown in Figures 1 and 2. Mean diffusivity of water is reduced at 2 hr post-blast and returns to normal at 24 hr and 7 days. Axial diffusivity appear...