Experimental and modeling studies of competitive Pb (II) and Cd (II) bioaccumulation by Aspergillus niger

Song, Xinwei; Li, Sensen; Zhu, Bihe; Chen, Yanting; Zhang, Lin; Li, Zhen

doi:10.1007/s00253-021-11497-3

Cited by 15 publications

(6 citation statements)

References 67 publications

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“…However, the tolerance of A. niger to Cd was much weaker due to its high toxicity and migration ( Wu et al, 2016 ). The biomass of A. niger was significantly lower under Cd stress than the CK and Pb treatments ( Wang, et al, 2017 ; Qiu, et al, 2021 ). The dead cell lysis would also release intracellular organic matters ( Li, et al, 2021 ), which were adsorbed around the living cells to isolate the contact between the cells and heavy metals.…”

Section: Discussionmentioning

confidence: 91%

“…Aspergillus niger strain information can be referred to our previous study ( Qiu et al, 2021 ). The fungus accession number in China General Microbiological Cultural Collection Center (CGMCC) is No.…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of Pb 2+ and Cd 2+ in the system were set based on the experimental design. The maximum concentration of H 2 PO 4 − was set to 10 mmol/L ( Qiu et al, 2021 ). The phase diagram of the dominant minerals was drawn by Act2 module.…”

Section: Methodsmentioning

confidence: 99%

“…The oxalic acid (H2C2O4) secreted by A. niger could efficiently precipitate heavy metal cations ( Yakout, 2014 ). In addition, heavy metals could be accumulated in both intracellular and extracellular regions of fungal cells ( Kapoor and Viraraghavan, 1997 ; Qiu et al, 2021 ; Geng et al, 2022 ). Thus, the filamentous fungus A. niger has been considered as an ideal strain for heavy metal bioremediation ( Ahluwalia and Goyal, 2007 ; Ren et al, 2009 ; Zegzouti et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress

Pan

Li²,

Wang³

et al. 2023

Front. Bioeng. Biotechnol.

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In the bioremediation process, coexistence of lead (Pb) and cadmium causes complex toxicity, resulting in the difficulty of bioremediation. This study investigated the physiological responses and bioaccumulation mechanisms of the typical filamentous fungus Aspergillus niger under the coexistence of Pb and Cd. Four treatments were set up, i.e., control, sole Pb, sole Cd, and coexistence of Pb and Cd. The morphology of A. niger were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Then, nano-scale secondary ion mass spectrometry (NanoSIMS) was applied to accurately investigate the distribution of heavy metals in the fungal cells under the coexistence of Pb and Cd. Finally, the metallogenic process and mineral types were simulated by Geochemist’s Workbench (GWB). The electron microscopic and NanoSIMS imaging showed that Pb and Cd were accumulated in both the extracellular and intracellular regions of the A. niger cells. In particular, the accumulated Pb content was ten times higher than that of Cd. However, Cd showed stronger toxicity than Pb to A. niger. Compared with the control treatment, Cd stress resulted in a two-fold increase of cell diameter and more extracellular substances, whereas the cell diameter increased nearly four times in the coexistence treatment. Moreover, the bioaccumulation of Pb was more intense than that of Cd during competitive sorption. The GWB simulation confirmed that Pb2+ can form multiple minerals (e.g., PbC2O4, PbHPO4, and Pb3(PO4)2, etc.), which significantly weakened its toxicity on the cell surface. This study elucidated the morphological characteristics of A. niger and competitive bioaccumulation under the coexistence of Pb and Cd, which would facilitate the application of microorganisms to the bioremediation of coexisted metals.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress

Pan

Li²,

Wang³

et al. 2023

Front. Bioeng. Biotechnol.

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“…There was a significant link between the copper level and Fusarium abundance in fresh chicken manure, composted chicken manure, and soil, showing that Fusarium has a higher Cu resistance (Elmer et al, 2021). Aspergillus has a high tolerance for heavy metals (Liu et al, 2020), which may be a result of enhanced activity of the pyruvate dehydrogenase and citrate synthetase enzymes as well as enhancement of Aspergillus’ TCA cycle (Qiu et al, 2021). Thus, the presence of Cd and Pb in chicken dung may enhance the development of Aspergillus .…”

Section: Discussionmentioning

confidence: 99%

Difference responses of soil fungal communities to cattle and chicken manure composting application

Zhang

Shen

Shang

et al. 2022

Journal of Applied Microbiology

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Aims Cattle and poultry manure composting are often applied on agricultural lands but the fungal community composition before and after application in soils is still unclear. This study describes soil fungal diversity after manure applications contribute to the correct resource use of livestock and poultry manures. Methods and Results Fresh manure samples were obtained from 10 beef cow farms and 12 egg‐laying poultry farms at five distinct phases of rearing. Surface soil samples were collected from vegetable plots within the farms after manure application at 15, 30 and 45 t hm−2. Using high‐throughput sequencing techniques, the ITS region was used to describe soil fungus populations. The fungal OTUs, Chao1 and ACE of cattle manure were relatively higher in the fattening stage (>12 months), the OTUs and ACE of chicken manure were the highest in the initial laying stage (16–24 weeks). The fungal diversity indices of vegetable soils had no linear change after cow or chicken manure application compared with the control. Ascomycota (84.7% of total sequences), Neocallimastigomycota (9.69%) and Basidiomycota (4.6%) were the dominant phyla in cattle manure. Ascomycota (88.9%) also predominated in chicken manure followed by Basidiomycota (8.9%). Following both cattle and chicken manure applications, the abundance of Ascomycota decreased, whereas Basidiomycota and Chytridiomycota increased in the soils. None of the dominant genus increased or decreased linearly with the increase of cattle and chicken composting application rate. The fungal‐dominant genera of the soils with and without manure composting application were mostly affected by soil pH and EC than manure. Pearson’s correlation analysis revealed that organic matter, Cu and Hg contents were strongly linked to the fungal diversity and the abundance of specific taxa in cattle manure. In chicken manure, OM, TN and Zn were major factors controlling the fungal diversity and community composition. Soil pH, EC and Cu, Zn, Cd, Hg and As content had pronounced effects on the beneficial and pathogenic genus in soil with and without manure composting. Beneficial fungal genus such as Aspergillus, Plectosphaerella, Acremonium, Meyerozyma and fungal pathogenic such as Fusarium, Cladosporium, Verticillium were sensitive to properties (EC, pH, OM) and heavy metals (Cu, Zn, Hg) contents of the environment relatively. This study can serve as an applicable contribution helping in farms management (especially to cattle and poultry breeding) and improving their resource use of livestock and poultry manures. Conclusions Soil heterogeneity rather than manure determines fungal communities in the vegetable fields, but we can encourage the sensible use of cattle and chicken manures in agroecosystems. Significance and Impact of the study This study will help farmers regulate the dosage of feed components which can increase the number of beneficial fungal genus or reduce the number of pathogenic fungal genus, improve their resource use of livestock and poultry manures and encourage t...

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Extracting extracellular polymeric substances from fungi in contrasts: from quantity to quality

Wang

et al. 2023

Appl Microbiol Biotechnol

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Experimental and modeling studies of competitive Pb (II) and Cd (II) bioaccumulation by Aspergillus niger

Cited by 15 publications

References 67 publications

Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress

Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress

Difference responses of soil fungal communities to cattle and chicken manure composting application

Extracting extracellular polymeric substances from fungi in contrasts: from quantity to quality

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