Experimental Alteration of Coupling Resistance at an Electrotonic Synapse

Asada, Yujiro; Bennett, Michael V. L.

doi:10.1083/jcb.49.1.159

Cited by 110 publications

(30 citation statements)

References 24 publications

(33 reference statements)

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“…The mechanical injury produced while cutting the axon to introduce the cannula did not uncouple the electrotonic junctions as reported by Asada & Bennett (1971). The difference in results is likely due to our cutting the axons as far as possible from the septal region (about 5-6 mm), while these authors cut it as close as possible (1-2 mm).…”

Section: Internal Perfusioncontrasting

confidence: 40%

Electrotonic coupling in internally perfused crayfish segmented axons.

Johnston¹,

Ramón²

1981

The Journal of Physiology

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SUMMARYWe have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused and non-perfused axons lead to the following conclusions:1. Internally perfused segmented axons behave very similarly to non-perfused axons.2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of axon.3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused axons.On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from both internally perfused and non-perfused axons.

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Section: Internal Perfusioncontrasting

confidence: 40%

Electrotonic coupling in internally perfused crayfish segmented axons.

Johnston¹,

Ramón²

1981

The Journal of Physiology

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“…Several investigators first implicated septate and gap junctions between invertebrate cells (26,27,33,50,54,55,72) and tight and gap junctions between vertebrate cells (I 1, 16,22,30,46,47,73,81) as avenues of such coupling. However, combined fine-structural and electrophysiological studies on the crayfish segmental axon presented compelling evidence that the gap junction is the site of low-resistance coupling (2,5,(52)(53)(54)(55). Other workers (28,36,64) have also found gap junctions and ionic coupling coexisting in cell cultures, affording further solid support for this association.…”

Section: Introductionmentioning

confidence: 97%

Assembly of Gap Junctions During Amphibian Neurulation

Decker

Friend

1974

The Journal of Cell Biology

242

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Sequential thin-section, tracer (K-pyroantimonate, lanthanum, ruthenium red, and horseradish peroxidase), and freeze-fracture studies were conducted on embryos and larvae of Rana pipiens to determine the steps involved in gap junction assembly during neurulation. The zonulae occludentes, which join contiguous neuroepithelial cells, fragment into solitary domains as the neural groove deepens. These plaque-like contacts also become permeable to a variety of tracers at this juncture. Where the ridges of these domains intersect, numerous 85-A participles apparently pile up against tight junctional remnants, creating arrays recognizable as gap junctions. With neural fold closure, the remaining tight junctional elements disappear and are replaced by macular gap junctions. Well below the junctional complex, gap junctions form independent of any visible, preexisting structure. Small, variegated clusters, containing 4-30 particles located in flat, particle-free regions, characterize this area. The number of particles within these arrays increases and they subsequently blend together into a polygonally packed aggregate resembling a gap junction. The assembly process in both apical and basal regions conforms with the concept of translational movement of particles within a fluid plasma membrane.

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“…13, 14) came as a surprise and remains a mystery. As far as we know there are no reports of cholinergic drugs interrupting electrical coupling between cells, but very little is known about how this type of intercellular communication can be modified (Asada & Bennett, 1971;Socolar & Politoff, 1971;Oliveira-Castro & 344 Lowenstein, 1971). The possibility exists that these agents are acting at chemical synapses; however, there is insufficient evidence to claim a role of ACh in horizontalcell/horizontal-cell communication.…”

Section: The Horizontal Cell Unit and Couplingmentioning

confidence: 99%

Synaptic transmission to the horizontal cells in the retina of the larval tiger salamander.

Marshall¹,

Werblin²

1978

The Journal of Physiology

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SUMMARY1. The receptive field diameter for most horizontal cells far exceeds the lateral spread of processes for any cell. Therefore horizontal cells probably receive synaptic input from neighbours as well as from the photoreceptors. The electrical effects of these two synaptic inputs were studied.2. We have characterized the electrical properties of the horizontal cell inputs by determining the current-voltage curves in dark and light. These curves were compared with those obtained in the presence of blockers of synaptic transmission, and during the application of some putative transmitter agents.3. The light response of the cells with narrow receptive fields, near 0 5 mm in diameter, was associated with a conductance decrease. The input resistance was typically 25 MQ, and increased to about 33 MK2 during the light response. The current-voltage curves in light and dark intersected near + 50 mV. The currentvoltage curve in the presence of C02+ or Mg2+ was nearly identical to the curve in the light.4. The putative transmitter substances glutamate, aspartate and GABA depolarized the cells by increasing conductance. Current-voltage curves measured in the presence of these substances intersected the dark and light curves at + 50 mV, the same level at which the dark and light curves intersect.5. The light response of cells with broad receptive fields, between 1.0 and 2*0 mm, showed little or no change in conductance associated with the light response. The input resistance was near 20 MQ, and the current-voltage curves intersected at an extrapolated potential level near 200 mV.6. In the presence of ACh, electrical properties of the broad field cells reverted to those of the narrow field cells: the receptive field was reduced to 0 5 mm, the input resistance increased, and the current-voltage curves intersected near +50 mV. Thus ACh appeared to interrupt synaptic input from neighbouring horizontal cells.7. The results confirm the suggestion that horizontal cells receive a tonic excitatory input from the photoreceptors which is decreased by light. They show that horizontal cells receive an additional input from their neighbours, not associated with a measurable conductance change. The input from neighbours is selectively inter-* Present address:

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Experimental Alteration of Coupling Resistance at an Electrotonic Synapse

Cited by 110 publications

References 24 publications

Electrotonic coupling in internally perfused crayfish segmented axons.

Electrotonic coupling in internally perfused crayfish segmented axons.

Assembly of Gap Junctions During Amphibian Neurulation

Synaptic transmission to the horizontal cells in the retina of the larval tiger salamander.

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