The purpose of this study was to develop and evaluate the effectiveness of the course on Global Health Nursing for Indian nursing students, intended to introduce the course as a selective course in the nursing curriculum. Methods: A quasi-experimental nonequivalent control group pre- and post-test design was carried out. The study participants were fifty final grade nursing students, considered as an experimental (n = 25) and control group (n = 25). The participants recruited by purposive sampling were fifty 4th grade nursing students (experimental 25, control 25) at St. X College of Nursing, India. The course on Global Health Nursing was developed by using the ADDIE model, and the duration of the course was about 16 h over three weeks. The course was implemented based on Gagné’s instructional method. Data were collected by self-perceived scales of Global Health Nursing before and after the course, the participants’ opinions, and feedback providing comments about the course. Results: The study results showed improvement in knowledge (F = 8.48; p < 0.001), skills (F = 96.14; p < 0.001), and performance on Global Health Nursing (F = 5.50; p < 0.001) and was statistically significant. Moreover, the participants described that they were satisfied with the quality of teaching–learning and achieved the learning goals. Conclusion: The outcome of this study could be extended to the existing nursing curriculum and would be modified to formulate a standard module in the curriculum of the Indian nursing system. The study offers implications for different fields of nursing, including nursing education, research, and practice in India.