Experience with therapeutic drug monitoring of three antifungal agents using an LC-MS/MS method in routine clinical practice

Yoon, Sun Joo; Lee, Kyung-Hoon; Oh, Jongwon; Woo, Hye In; Lee, Soo-Youn

doi:10.1016/j.clinbiochem.2019.06.003

Cited by 19 publications

(12 citation statements)

References 18 publications

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“…The calibration curve of each analyte showed good linearity in the target concentration range, and the correlation coefficient (r) was in the range of 0.991-0.999. The linearity and quantification range for all compounds used in the study were similar to those previously reported (Dasgupta, 2016;Yoon et al, 2019), and they were sufficient for TDM in clinical practice. The retention time for each analysis target was less than 2 min (Figure 2), could be measured quickly, and could be applied to daily clinical practice.…”

Section: Validationsupporting

confidence: 79%

“…Validation was performed in accordance with the FDA guidelines for Validation of Bioassays (US Food and Drug Administration, 2018). For validation, calibration standards (CSs) and quality controls (QCs) were prepared using ACN/methanol (90/10, v/v), and they were stored at −20 C. The CS and QC samples were prepared as previously described (Table 2, Dasgupta, 2016;Yoon et al, 2019). Subsequently, 80 μL of IS solution and 80 μL of CS or QC were added to 40 μL of plasma, and the same procedure as that of sample pre-treatment was performed.…”

Section: Validationmentioning

confidence: 99%

“…Blood concentrations of these drugs are measured using immunoassay, HPLC with ultraviolet detection, LC-MS, and LC-tandem mass spectrometry (LC-MS/MS) (Ashbee et al, 2014;Hamada et al, 2013;Yang & Wang, 2008). In clinical practice, along with high accuracy and high sensitivity, a simple and rapid analysis method is required, and a method for the simultaneous analysis of immunosuppressive and antifungal drugs using LC-MS/MS has been developed in recent years (Gong et al, 2019;Jenkins, Black, & Schneider, 2018;Karapirli et al, 2012;Yoon, Lee, Oh, Woo, & Lee, 2019). However, these methods can only analyze drugs belonging to the same class, such as immunosuppressive or antifungal drugs.…”

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confidence: 99%

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Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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Several drugs are administered to lung‐transplanted patients, which are monitored using therapeutic drug monitoring (TDM). Therefore, we developed and validated a liquid chromatography–tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. Chromatographic separation was achieved using a C18 column and gradient flow of mobile phase comprising 20 mM aqueous ammonium formate and 20 mM ammonium formate‐methanol solution. A simple protein precipitation treatment was performed using acetonitrile/methanol and mycophenolic acid‐2H3, voriconazole‐2H3, itraconazole‐2H4, and hydroxyitraconazole‐2H4 as internal standards. The linearity ranges of mycophenolic acid, voriconazole, itraconazole, and hydroxyitraconazole were 100–20,000, 50–10,000, 5–1000, and 5–1000 ng/mL, respectively. The retention time of each target was less than 2 min. The relative errors in intra‐ and inter‐day were within ±7.6%, the coefficient of variation was 8.9% or less for quality control low, medium, and high, and it was 15.8% or less for lower limit of quantitation. Moreover, the patient samples were successfully quantified, and they were within the linear range of measurements. Therefore, our new method may be useful for TDM in lung‐transplanted patients.

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Section: Validationsupporting

confidence: 79%

Section: Validationmentioning

confidence: 99%

mentioning

confidence: 99%

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Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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“…Present-day TDM involves processes that are far from ideal for routine monitoring due to their invasive, time-consuming, expensive, and specialist nature [71]. Biosensors are thus being developed to act as non-invasive platforms for real-time TDM of NTI drugs, with applications including vancomycin (Figure 3E) [72],…”

Section: Sensors Of the Futurementioning

confidence: 99%

Connected healthcare: Improving patient care using digital health technologies

Awad

Trenfield

Pollard

et al. 2021

Advanced Drug Delivery Reviews

184

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“…Several reports have outlined various methods to determine plasma voriconazole concentration using high-performance liquid chromatography ultraviolet (HPLC-UV) [ 12 , 13 , 14 , 15 , 16 ] and liquid chromatography with tandem mass spectrometry (LC-MS/MS) [ 17 , 18 , 19 , 20 , 21 ] Globally, HPLC-UV instruments are more widely used than LC-MS/MS instruments. Furthermore, HPLC-UV setups have low initial costs compared with LC-MS/MS setups.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Liquid Chromatography for Ultra-Simple Determination of Plasma Voriconazole Concentration

Yasu

Nomura

Gando

et al. 2022

JoF

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Voriconazole is an antifungal drug used to treat invasive aspergillosis. Voriconazole exhibits nonlinear behavior and considerable individual variability in its pharmacokinetic profile. Invasive aspergillosis has a poor prognosis, and failure of treatment owing to low voriconazole blood levels is undesirable. Thus, therapeutic drug monitoring (TDM) of voriconazole is recommended. However, plasma voriconazole concentration is rarely measured in hospitals, and the TDM of voriconazole is not widely practiced in Japan. We aimed to develop an ultra-simple method to measure plasma voriconazole concentration. Ten microliters of plasma sample was extracted, and proteins were precipitated using methanol extraction. Voriconazole and ketoconazole (internal standard) were separated using high-performance liquid chromatography. A calibration curve was prepared, which was linear over plasma voriconazole concentrations of 0.125–12.5 µg/mL, with a coefficient of determination of 0.9999. The intra-day and inter-day validation coefficients were 0.9%–2.2% and 1.3%–6.1%, respectively. The assay accuracy was −4.2% to 1.6%, and recovery was > 97.8%. Our ultra-simple, sensitive, and inexpensive high-performance liquid chromatography ultraviolet method to determine plasma voriconazole concentration will help improve the voriconazole TDM implementation rate and contribute to effective and safe voriconazole use.

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Experience with therapeutic drug monitoring of three antifungal agents using an LC-MS/MS method in routine clinical practice

Cited by 19 publications

References 18 publications

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Connected healthcare: Improving patient care using digital health technologies

High-Performance Liquid Chromatography for Ultra-Simple Determination of Plasma Voriconazole Concentration

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