Experience with therapeutic drug monitoring of three antifungal agents using an LC-MS/MS method in routine clinical practice

Yoon, Sun Joo; Lee, K.H.; Oh, Jongwook; Woo, Hye In; Lee, S.Y.

doi:10.1016/j.cca.2019.03.162

Cited by 3 publications

(5 citation statements)

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“…The calibration curve of each analyte showed good linearity in the target concentration range, and the correlation coefficient ( r ) was in the range of 0.991–0.999. The linearity and quantification range for all compounds used in the study were similar to those previously reported (Dasgupta, 2016; Yoon et al, 2019), and they were sufficient for TDM in clinical practice. The retention time for each analysis target was less than 2 min (Figure 2), could be measured quickly, and could be applied to daily clinical practice.…”

Section: Resultssupporting

confidence: 85%

“…For validation, calibration standards (CSs) and quality controls (QCs) were prepared using ACN/methanol (90/10, v/v), and they were stored at −20°C. The CS and QC samples were prepared as previously described (Table 2, Dasgupta, 2016; Yoon et al, 2019). Subsequently, 80 μL of IS solution and 80 μL of CS or QC were added to 40 μL of plasma, and the same procedure as that of sample pre‐treatment was performed.…”

Section: Methodsmentioning

confidence: 99%

“…Blood concentrations of these drugs are measured using immunoassay, HPLC with ultraviolet detection, LC–MS, and LC‐tandem mass spectrometry (LC–MS/MS) (Ashbee et al, 2014; Hamada et al, 2013; Yang & Wang, 2008). In clinical practice, along with high accuracy and high sensitivity, a simple and rapid analysis method is required, and a method for the simultaneous analysis of immunosuppressive and antifungal drugs using LC–MS/MS has been developed in recent years (Gong et al, 2019; Jenkins, Black, & Schneider, 2018; Karapirli et al, 2012; Yoon, Lee, Oh, Woo, & Lee, 2019). However, these methods can only analyze drugs belonging to the same class, such as immunosuppressive or antifungal drugs.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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Several drugs are administered to lung‐transplanted patients, which are monitored using therapeutic drug monitoring (TDM). Therefore, we developed and validated a liquid chromatography–tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. Chromatographic separation was achieved using a C18 column and gradient flow of mobile phase comprising 20 mM aqueous ammonium formate and 20 mM ammonium formate‐methanol solution. A simple protein precipitation treatment was performed using acetonitrile/methanol and mycophenolic acid‐2H3, voriconazole‐2H3, itraconazole‐2H4, and hydroxyitraconazole‐2H4 as internal standards. The linearity ranges of mycophenolic acid, voriconazole, itraconazole, and hydroxyitraconazole were 100–20,000, 50–10,000, 5–1000, and 5–1000 ng/mL, respectively. The retention time of each target was less than 2 min. The relative errors in intra‐ and inter‐day were within ±7.6%, the coefficient of variation was 8.9% or less for quality control low, medium, and high, and it was 15.8% or less for lower limit of quantitation. Moreover, the patient samples were successfully quantified, and they were within the linear range of measurements. Therefore, our new method may be useful for TDM in lung‐transplanted patients.

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Section: Resultssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Takasaki

Hirasawa

Sato

et al. 2021

Biomedical Chromatography

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“…Since voriconazole, itraconazole, and posaconazole show a high level of intra-and interindividual variability and a narrow therapeutic window, antifungal TDM is generally indicated for these agents. 4,5 In a previous study isavuconazole was shown to have a high bioavailability and dose-proportional pharmacokinetic, which demonstrated no relevant differences between patients with IFD and healthy subjects. 6 Several methods have been established for the quantification of serum concentrations of antifungal agents, including liquid chromatography-tandem mass-spectrometry (LC-MS/MS), high-performance liquid chromatography (HPLC), and the bioassay.…”

Section: Introductionmentioning

confidence: 97%

Implementation of a Dual-Column Liquid Chromatography-Tandem Mass-Spectrometry Method for the Quantification of Isavuconazole in Clinical Practice

et al. 2021

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Objectives Therapeutic drug monitoring (TDM) of isavuconazole, which is a novel broad-spectrum antimycoticum against invasive fungal infections, ensures an effective exposure of the drug and minimizes the risk of toxicity. This study is aimed at evaluating the analytical performance of a dual-column liquid chromatography-tandem mass-spectrometry (LC-MS/MS) method for isavuconazole quantification. Materials and Methods The method was performed on a Voyager TSQ Quantum triple quadrupole instrument equipped with an Ultimate 3000 chromatography system (Thermo Fisher Scientific, San Jose, California, United States). Analytical and preanalytical requirements of the isavuconazole LC-MS/MS method were evaluated. Sample stability measurements were performed at room temperature (RT) and in serum tubes with separator gel. Results The isavuconazole LC-MS/MS method was linear over the concentration range of 0.2 to 12.8 mg/L. The coefficient of determination (r 2) always exceeded 0.999. Within- and between-run precision ranged between 1.4 to 2.9% and 1.5 to 3.0%, the recovery between 93.9 and 102.7%. At RT, serum samples were stable for 3 days. Isavuconazole serum concentrations were significantly lower after incubation (18 hours) in serum tubes with separator gel at RT. Conclusion The dual-column isavuconazole LC-MS/MS is a reliable tool for the TDM of isavuconazole. Serum samples are stable for at least 3 days and should be collected in tubes without separator gel.

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“…Accordingly, there has been a significant effort to increase the throughput of chromatographic methods [10]. Pioneering multiplex approaches have been reported for antiretroviral agents (ARVs) [11], antifungals [12], antineoplastics [13], antibiotics [6,7,14], antidepressants [15], and immunosuppressive drugs [16] in the past decade. More recently, ultra-performance liquid chromatography (UPLC)-MS/MS has been used for simultaneous quantification of antibiotics [17] and ARVs from plasma [11] and breast milk samples [18].…”

mentioning

confidence: 99%

On-Site Therapeutic Drug Monitoring

Ates

Roberts

Lipman

et al. 2020

Trends in Biotechnology

161

160

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Trends in Biotechnology mass spectrometry (LC-MS/MS) (see Glossary) methods. Despite its high specificity, matrix interference may lead to falsely low or high results; that is, the matrix and co-eluting compounds can interfere with the ionization process in MS (via ion suppression/enhancement) [5]. Furthermore, the throughput of LC-MS/MS is lower than that of the conventional immunoassay platforms. Recent studies are either addressing these issues [6,7] or exploiting or improving this method's inherent advantages [8,9]. Accordingly, there has been a significant effort to increase the throughput of chromatographic methods [10]. Pioneering multiplex approaches have been reported for antiretroviral agents (ARVs) [11], antifungals [12], antineoplastics [13], antibiotics [6,7,14], antidepressants [15], and immunosuppressive drugs [16] in the past decade. More recently, ultra-performance liquid chromatography (UPLC)-MS/MS has been used for simultaneous quantification of antibiotics [17] and ARVs from plasma [11] and breast milk samples [18]. Another focus is the extension of TDM studies toward unconventional samples and sampling. Several LC-MS/MS methods have been developed and applied for hair [11,18], dried blood spots [19], urine [20], sweat [21], saliva [22,23], and tissue biopsies [24].

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Experience with therapeutic drug monitoring of three antifungal agents using an LC-MS/MS method in routine clinical practice

Cited by 3 publications

References 0 publications

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring

Implementation of a Dual-Column Liquid Chromatography-Tandem Mass-Spectrometry Method for the Quantification of Isavuconazole in Clinical Practice

On-Site Therapeutic Drug Monitoring

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