Experience with routine applications of liquid chromatography—mass spectrometry in the pharmaceutical industry

Schellenberg, K.; Linder, Markus.; Groeppelin, A.; Erni, F.

doi:10.1016/s0021-9673(01)94176-7

Cited by 24 publications

(6 citation statements)

References 2 publications

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“…Chromatographic (LC or GC) separations combined with MS detection have been widely used for qualitative and quantitative analysis, especially for small molecules in pharmaceutical research [27,106,107]. Mass spectrometry provides excellent selectivity by detecting signals related to the MW of the analyte.…”

Section: Mass Spectrometric Detectionmentioning

confidence: 99%

Analytical procedures for quantification of peptides in pharmaceutical research by liquid chromatography?mass spectrometry

John¹,

Walden²,

Schäfer³

et al. 2004

Analytical and Bioanalytical Chemistry

155

104

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Peptide quantification by liquid chromatography-mass spectrometry (LC-MS) combines the high resolving power of reversed-phase (RP) chromatography with the excellent selectivity and sensitivity of mass spectrometric detection. On the basis of comprehensive practical experience in the analysis of small molecules, pharmaceutical research is developing technologies for analysis of a growing number of peptidic drug candidates. This article is a detailed review of procedures based on LC-MS techniques for quantitative determination of peptides. With the focus on pharmaceutical applications several technologies for sample preparation, various aspects of peptide chromatography, important characteristics of ESI-MS, selectivity of MS-detection modes, the large variability of internal standards, and modern instrumentation are discussed. The demand for reliable, robust, sensitive, and accurate methods is discussed using numerous examples from the literature, complemented by experiments and results from our laboratory.

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Section: Mass Spectrometric Detectionmentioning

confidence: 99%

Analytical procedures for quantification of peptides in pharmaceutical research by liquid chromatography?mass spectrometry

John¹,

Walden²,

Schäfer³

et al. 2004

Analytical and Bioanalytical Chemistry

155

104

View full text Add to dashboard Cite

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“…The direct liquid introduction interface has been used with some success for a number of years, but is no longer commercially available. This is primarily due to the high technical skill required to operate this interface and the frequently occurring problems with instability of the liquid jet especially at low (<15%) and high (>go%) water contents of the mobile phase (Kenyon et af., 1980(Kenyon et af., , 1981Erni, 1982;Schellenberg et al, 1987). With the direct liquid introduction interface, as well as with other interfaces that rely on solventmediated CI and/or ion evaporation (thermospray, heated nebulizer, ionspray and electrospray), the performance is highly dependent on the type of sample analyzed.…”

Section: Important Parameters Of Lc-ms Interfacesmentioning

confidence: 99%

“…Erni and co-workers (Kenyon et al, 1981;Erni, 1982) reported the analysis of ergot alkaloids by means of a DLI LC-MS. Bromocryptine was analyzed on a C,-type column using acetonitrile: water (6: 1) or acetonitrile : 0.01 M ammonium formate (35 : 65) as mobile phase. Bromocryptine and a series of byproducts were also analyzed on a C18 column with acetonitrile (gradient from 10-60%) : 0.01 M ammonium carbonate as eluent (Schellenberg et al, 1987). Acetonitrile gave better results than methanol or isopropanol because of the lower background signals and better sensitivity.…”

Section: -mentioning

confidence: 99%

Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

Verpoorte

Niessen

1994

Phytochemical Analysis

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“…(14) Griffiths, P.; de Haseth, J. Fourier Transform Infrared Spectroscopy, INTRODUCTION Liquid chromatography/mass spectrometry (LC/MS) facilitates analyses of polar, nonvolatile, and thermally labile compounds. The thermospray technique has proven to be a useful and practical methodology as an LC/MS interface (1)(2)(3)(4).…”

Section: Literature Citedmentioning

confidence: 99%

Enhanced analysis of poly(ethylene glycols) and peptides using thermospray mass spectrometry

Fink¹,

Freas²

1989

Anal. Chem.

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The effects of a needle-tip repeller electrode on thermospray mass spectra of poly(ethylene glycols) and peptide samples have been studied. A significant increase in ion current was observed for increased repeller potentials in the low-mass region (m/z 300 to m/z 1100) and the high-mass region (m/z 1100 to m/z 1900). The change in ion current is due to a change in ion extraction efficiency because of increased ion diffusion rates. The amount of peptide sample needed for direct injection studies was decreased 1 to 2 orders of magnitude by using an ion source with both a needle-tip repeller electrode and a restricted vaporizer probe tip.

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Experience with routine applications of liquid chromatography—mass spectrometry in the pharmaceutical industry

Cited by 24 publications

References 2 publications

Analytical procedures for quantification of peptides in pharmaceutical research by liquid chromatography?mass spectrometry

Analytical procedures for quantification of peptides in pharmaceutical research by liquid chromatography?mass spectrometry

Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

Enhanced analysis of poly(ethylene glycols) and peptides using thermospray mass spectrometry

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