Experience with Rast-Based Immunotherapy

Fungus‐Specific IgG and IgE in Allergic Fungal Rhinosinusitis

“…As a simpler means of accomplishing this, serum fungal sIgG and sIgE can be measured by radioallergosorbent test (RAST). In vitro testing via modified RAST is an accepted standard in screening and diagnosing inhalant allergy, including molds 13–15 . Unfortunately, there have been no controlled studies to examine the diagnostic efficacy of these values for AFS.…”

“…In vitro testing via modified RAST is an accepted standard in screening and diagnosing inhalant allergy, including molds. [13][14][15] Unfortunately, there have been no controlled studies to examine the diagnostic efficacy of these values for AFS.…”

Immunotherapy Basics

“…In vitro tests have the added advantage of convenience but are otherwise comparable with the in vivo tests. A discussion of the evolution of in vitro tests and a comparison with in vivo tests has been published 46 …”

“…A discussion of the evolution of in vitro tests and a comparison with in vivo tests has been published. 46 All four testing methods are sensitive and safe. The in vivo tests are safe because they start testing with a low screening allergen dose, whereas the in vitro tests are safe because only drawing of a blood sample is required.…”

Monoplex and multiplex immunoassays: approval, advancements, and alternatives

“…Nowadays, it is also used in screening for immunity, allergens in the food industry, or molecular biology laboratories. A few variants of the RIA method are also employed that enlarge the range of applications such as immunoradiometric assay (IRMA), radioallergosorbent test (RAST), and Farr assay (dsDNA-Ab RIA) which are employed for the detection of anti-double stranded DNA (anti-dsDNA) antibodies in autoimmune diseases (Boguszewska et al 2019 ; Smeenk et al 1991 , 1990 ; Derksen et al 2002 ; Fadal 1992 ; Miles 1975 ).…”