Experience of stem cell use in treatment of skin burns

Abstract

The results of three-year research on the use of allogeneic mesenchymal stem cells of adipose tissue (AMSCs) in the treatment of skin burns of II-III degree are presented. in a complex with wounds dressing of nanofibers chitosan and copolyamide, hyaluronic acid. It was found that with surgical necrectomy, introduction of AMSCs and substitution of defects with natural polymer coatings, the healing time is reduced by 89% (p < 0.05). Isolated administration of MSC reduces the healing period by no more than 5% (p > 0.05). The combined use of wounds dressings of nanofibers chitosan and copo lyamide with MSC accelerates the regeneration process by 26% (p < 0.05), with the introduction of AMSCs accelerating the development of granulation tissue by the fifth day of observation by 83% (p < 0.01). Joint use of wound coverings on the basis of hyaluronic acid with AMSCs is accompanied by an increase in the number of vessels of the microcirculatory bed in the defect area by 185% (p < 0.01). Clinical evaluation of the effectiveness of drugs with stem cells – a gel for topical application and a suspension of MSC LC for injection administration demonstrate their ability to optimize regeneration in the burn zone. Application of gel with AMSCs reduces the duration of epithelialization of border (dermal) burns by 2.2-2.4 times, with the final healing period being reduced by 59% (p < 0.01) and the suppuration frequency by 30% (p < 0.05). The introduction of a suspension of AMSCs into the zone of deep burn increases the frequency of engraftment of autografts, stimulates angiogenesis and proliferation of fibroblasts in the superficial and deep layers of the dermis. In the area of MSC administration, the LC perfusion level and the amplitude of blood flow fluctuation are twice as high as the values in the zones without the introduction of cells.

Principais evidências clínicas da terapia com células-tronco na cicatrização de queimaduras: uma revisão sistemática

“…À luz dos resultados, verificou-se que a terapia com células-tronco melhorou significativamente a taxa de cicatrização de queimaduras, independentemente do tipo de transplante, área da queimadura e método de tratamento (Rasulov et al, 2005;Mansilla et al, 2015;Zinovyev et al, 2018;Zhou et al, 2022).…”

“…Dois tipos de células-tronco foram utilizados nos estudos: células-tronco derivadas da medula óssea (BMSC) (Rasulov et al, 2005;Mansilla et al, 2015); células-tronco derivadas do tecido adiposo (ASC) (Zinovyev et al, 2018;Zhou et al, 2022). As BMSC eram tradicionalmente a principal fonte de células-tronco mesenquimais adultas, porém sua coleta era Research, Society and Development, v. 11, n. 10, e184111032781, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i10.32781 invasiva com baixos rendimentos.…”

Evaluation of the Efficacy of Treatment of 3rd Degree Burns With Hldf6 Peptide and Silver Nanoparticles in Carbopol 2020 Gel in Vivo Experiment

“…Принятый стандарт ранней некрэктомии при глубоких термических ожогах кожи делает необходимым поиск новых средств, позволяющих активизировать процессы заживления ран и сократить общие сроки их лечения [1,13]. Как наиболее перспективные новые методики лечения поверхностных и глубоких ожоговых поражений можно выделить биотехнологические [10], в том числе клеточные продукты [24], комплексные тканеинженерные конструкции на основе биополимерных матриксов [23], заселяемые алло-и аутогенными клеточными культурами [13].…”