Expedient synthesis and luminescence sensing of the inositol pyrophosphate cellular messenger 5-PP-InsP₅

Abstract

Inositol pyrophosphates are important biomolecules associated with apoptosis, cell growth and kinase regulation, yet their exact biological roles are still emerging and probes do not exist for their selective detection....

Fluorescent ratiometric supramolecular tandem assays for phosphatase and phytase enzymes

“…30 The literature contains many fluorescence assays that track consumption of ATP but only a small fraction of them produce a ratiometric response. 4,[31][32][33][34][35][36][37] In Fig. 4a is a schematic summary of our continuous ratiometric AP assay.…”

A dual encapsulation strategy to generate anion-responsive luminescent lanthanide hydrogels

“…24,25 Complex [Eu.1] + serves as an appropriate model for encapsulation and represents a family of lanthanide-based sensors that respond to biological anions. [26][27][28][29] The synthesis of the particles begins with formation of hydrophilic polymer p(OEGMEM) 75 (Scheme S1, ESI †) and subsequent chain extension of the hydrophilic shell (Scheme S2, ESI †) according to our previously disclosed procedures. 20,22 Full details of the polymer synthesis and characterisation are provided in the ESI.…”

“…24,25 Complex [Eu.1] + serves as an appropriate model for encapsulation and represents a family of lanthanide-based sensors that respond to biological anions. 26–29…”

pH-Dependent phosphate separation using a tripodal hexaurea receptor

“…, urea, amide, amine) and demonstrated selective phosphate binding in bulk solution. 9–19 However, the removal of phosphates from aqueous solution using synthetic receptors remains a daunting challenge. This is because of the complex acid–base properties (p K a of H 3 PO 4 : 2.1, 7.2, 12.3) and extremely high hydration energies (Δ G hyd : −473 kJ mol −1 for H 2 PO 4 − , −1366 kJ mol −1 for HPO 4 2− , and −2753 kJ mol −1 for PO 4 3− ) as well as the inevitable self-association of phosphates.…”

“…7,8 Accordingly, many synthetic receptors have been designed by programming with hydrogen bonding motifs (e.g., urea, amide, amine) and demonstrated selective phosphate binding in bulk solution. [9][10][11][12][13][14][15][16][17][18][19] However, the removal of phosphates from aqueous solution using synthetic receptors remains a daunting challenge. This is because of the complex acid-base properties (pK a of H , and À2753 kJ mol À1 for PO 4 3À ) as well as the inevitable self-association of phosphates.…”