Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue

Winnie Hung, Yik Ling; Xie, Chengyi; Wang, Jianing; Diao, Xin; Li, Ruxin; Wang, Xiaoxiao; Qiu, Shulan; Fang, Jiacheng; Cai, Zongwei

doi:10.1101/2023.08.28.555097

Cited by 4 publications

(1 citation statement)

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“…I am reminded of the eloquent words of Ken Busch, who opined that “The evolution of MS has been punctuated by new instruments produced to meet new analytical needs, changing the shape or starting entirely new S‐curves.” 181 Each new iteration and application of a tool has the potential to birth a brand‐new field or subfield, placing us again at the exciting threshold of a brand new curve of development. Recent innovations enabling, for example, glycan detection, 74,77,182–184 online isomer differentiation, 185–200 tissue expansion for high spatial resolution, 201,202 and combining images from multiple modalities 203–206 hold promise for opening new chapters in imaging with mass spectrometry.…”

Section: Analytical Figures Of Meritmentioning

confidence: 99%

Imaging with mass spectrometry: Which ionization technique is best?

Prentice

2024

J Mass Spectrom

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The use of mass spectrometry (MS) to acquire molecular images of biological tissues and other substrates has developed into an indispensable analytical tool over the past 25 years. Imaging mass spectrometry technologies are widely used today to study the in situ spatial distributions for a variety of analytes. Early MS images were acquired using secondary ion mass spectrometry and matrix‐assisted laser desorption/ionization. Researchers have also designed and developed other ionization techniques in recent years to probe surfaces and generate MS images, including desorption electrospray ionization (DESI), nanoDESI, laser ablation electrospray ionization, and infrared matrix‐assisted laser desorption electrospray ionization. Investigators now have a plethora of ionization techniques to select from when performing imaging mass spectrometry experiments. This brief perspective will highlight the utility and relative figures of merit of these techniques within the context of their use in imaging mass spectrometry.

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Section: Analytical Figures Of Meritmentioning

confidence: 99%

Imaging with mass spectrometry: Which ionization technique is best?

Prentice

2024

J Mass Spectrom

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Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level

Chadha,

Guerrero,

Wei

et al. 2024

ACS Cent. Sci.

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This outlook explores how two different molecular imaging approaches might be combined to gain insight into dynamic, subcellular metabolic processes. Specifically, we discuss how matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and stimulated Raman scattering (SRS) microscopy, which have significantly pushed the boundaries of imaging metabolic and metabolomic analyses in their own right, could be combined to create comprehensive molecular images. We first briefly summarize the recent advances for each technique. We then explore how one might overcome the inherent limitations of each individual method, by envisioning orthogonal and interchangeable workflows. Additionally, we delve into the potential benefits of adopting a complementary approach that combines both MSI and SRS spectro-microscopy for informing on specific chemical structures through functional-group-specific targets. Ultimately, by integrating the strengths of both imaging modalities, researchers can achieve a more comprehensive understanding of biological and chemical systems, enabling precise metabolic investigations. This synergistic approach holds substantial promise to expand our toolkit for studying metabolites in complex environments.

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Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissues

Zhang,

Wu,

Shen

et al. 2024

Chemical & Biomedical Imaging

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The quest for high spatial resolution in molecular identification is critical across various domains, including physiology, pathology, and pharmaceutical research. Super-resolution microscopy has made strides by surpassing the Abbe diffraction limit, but it relies on sophisticated equipment and is limited by the sample size to handle. Expansion microscopy, an emerging technique, has broadened the scope of subdiffraction imaging. It chemically preserves tissues at a large scale and physically enlarges them 4−20 times linearly, enabling superresolution observation. This review begins by exploring the foundational concepts of tissue clearing and the latest methodologies in the field. It then delves into the core tenets of expansion microscopy, covering a range of protocols. The review spotlights advancements in enhancing resolution, improving labeling efficiency, and ensuring isotropic tissue expansion. Finally, the review offers insights into the prospective evolution of expansion microscopy. It emphasizes the potential role of machine learning in refining image quality and in the autonomous extraction of data, which could revolutionize the way we visualize and understand biological tissues.

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Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue

Cited by 4 publications

References 50 publications

Imaging with mass spectrometry: Which ionization technique is best?

Imaging with mass spectrometry: Which ionization technique is best?

Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level

Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissues

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