Expansion of the γδ T cell subset in vivo during bloodstage malaria in B cell-deficient mice

Abstract

Mice rendered B cell-deficient either by J. Leukoc. Biol. 60: 221-229; 1996.

Splenic γδ T Cells Regulated by CD4+T Cells Are Required To Control ChronicPlasmodium chabaudiMalaria in the B-Cell-Deficient Mouse

“…Results from studies of mouse models of malaria also support the role of ␥␦ T cells as effector cells, as well as their regulation by CD4 ϩ T cells (42)(43)(44). Splenic ␥␦ T cells increase ϳ100-fold in the spleens of B-cell-deficient J H Ϫ/Ϫ mice infected with different murine species of Plasmodium (43).…”

“…However, the proliferative response of these ␥␦ T cells in CD4 ϩ T-celldepleted peripheral blood mononuclear cells is restored by the addition of exogenous cytokines that signal through components of the interleukin-2 receptor (IL-2R), such as IL-2, IL-4, and IL-15 (10,12). In addition, human ␥␦ T cells from naïve donors inhibit the replication of P. falciparum in vitro (11,43), possibly by granulysin-mediated mechanisms (13). Taken together these findings suggest that ␥␦ T cells respond to malarial antigens and contribute to protection against malaria by killing blood-stage parasites.…”

“…Splenic ␥␦ T cells increase ϳ100-fold in the spleens of B-cell-deficient J H Ϫ/Ϫ mice infected with different murine species of Plasmodium (43). CD4 ϩ T cells are required for the splenic ␥␦ T-cell response to occur in vivo during Plasmodium chabaudi malaria in these mice (36,47).…”

“…CD4 ϩ T cells are required for the splenic ␥␦ T-cell response to occur in vivo during Plasmodium chabaudi malaria in these mice (36,47). During acute P. chabaudi malaria, the splenic ␥␦ T-cell population begins to expand in the ascending phase of parasitemia before reaching peak values in the descending phase, and it remains elevated for weeks thereafter (43). Splenic ␥␦ T cells from P. chabaudi-infected mice express CD25, CD44, and CD71 transiently, with maximal levels on day 10 of infection, but maintain their expression of the ␥␦ T-cell activation marker B220 (8,43).…”

“…During acute P. chabaudi malaria, the splenic ␥␦ T-cell population begins to expand in the ascending phase of parasitemia before reaching peak values in the descending phase, and it remains elevated for weeks thereafter (43). Splenic ␥␦ T cells from P. chabaudi-infected mice express CD25, CD44, and CD71 transiently, with maximal levels on day 10 of infection, but maintain their expression of the ␥␦ T-cell activation marker B220 (8,43). Cell cycle analysis indicates that once maximal expansion of the ␥␦ T-cell population occurs, proliferation then declines markedly, with only 15% of the cells exhibiting Ͼ1N DNA content (43).…”