Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymeraseElectronic supplementary information (ESI) available: sequencing of the PCR products (108mer DNA) from substrates 1 and 2. See http://www.rsc.org/suppdata/cc/b1/b107838k/

PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis

“…Before studying the enzymatic incorporation of POM–dNTP conjugates during the polymerase chain reaction (PCR), we evaluated the accessible concentration range for PCR in the presence of Keggin and Dawson POMs. The KAPA2G Robust DNA polymerase (markedly less expensive than the commonly used KOD XL DNA polymerase) was used in the presence of increasing amounts of [SiW 11 O 39 {Sn(CH 2 ) 2 COOH)}] 5− and [P 2 W 17 O 61 {Sn(CH 2 ) 2 COOH)}] 7− for the PCR amplification of a DNA sequence specific for the potential biowarfare agent Yersinia pestis . Whereas the Keggin compound can be present up to 100 μ m , the Dawson compound at more than 2 μ m inhibits the PCR (Figure S9a).…”

In vitro selection of BNA (LNA) aptamers

“…Previously, we demonstrated the usefulness of KOD DNA polymerases in enzymatic syntheses of base-modified DNAs and investigated their application in SELEX methods since 2001. [66][67][68][69][70][71][72][73][74][75][76][77][78] Therefore, we readily noticed that KOD-related DNA polymerases are also applicable DNA synthesis involving modified sugars. This knowledge is now being widely accepted among researchers engaged in relevant studies.…”

Nucleic Acid with Guanidinium Modification Exhibits Efficient Cellular Uptake

“…After 48 hours, cells incubated with core-Tg 4 had higher fluorescence than a population of cells incubated in the absence of core-Tg 4 . To confirm the role of the guanidinium group, we synthesized 5-[(6-aminohexylcarbamoyl)methyl]-2'-deoxyuridine (Ta), [25] which has a Lys side chain (Supporting Information); it is known that Arg peptides undergo cellular uptake more readily than Lys peptides. [26,27] If the cellular uptake does not depend on the guanidinium group, then one would expect the cellular uptake efficiency of Tg-and Tamodified DNA to be the same.…”