Expansion of <scp>CD</scp>4 phenotype among <scp>CD</scp>160 receptor‐expressing lymphocytes in murine pregnancy

Meggyes, Mátyás; Szereday, László; Jaksó, Pál; Bogar, Barbara; Bogdan, A. T.; Nörenberg, Jasper; Mikó, Éva; Barakonyi, Alíz

doi:10.1111/aji.12745

Cited by 4 publications

(8 citation statements)

References 32 publications

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“…According to its FPKM profile, the CD160 protein is expressed in the oocyte. There are only a few studies on the function of CD160 in the female reproductive tract, such as one which explores CD160 s role in murine pregnancies [35]. However, the Cd160 KO mice were fertile, indicating that Cd160 may only play a role in immunological situations.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice

Abbasi

Kodani

Emori

et al. 2020

Cells

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There are over 200 genes that are predicted to be solely expressed in the oocyte and ovary, and thousands more that have expression patterns in the female reproductive tract. Unfortunately, many of their physiological functions, such as their roles in oogenesis or fertilization, have yet to be elucidated. Previous knockout (KO) mice studies have proven that many of the genes that were once thought to be essential for fertility are dispensable in vivo. Therefore, it is extremely important to confirm the roles of all genes before spending immense time studying them in vitro. To do this, our laboratory analyzes the functions of ovary and oocyte-enriched genes in vivo through generating CRISPR/Cas9 KO mice and examining their fertility. In this study, we have knocked out three Oosp family genes (Oosp1, Oosp2, and Oosp3) that have expression patterns linked to the female reproductive system and found that the triple KO (TKO) mutant mice generated exhibited decreased prolificacy but were not infertile; thus, these genes may potentially be dispensable for fertility. We also generated Cd160 and Egfl6 KO mice and found these genes are individually dispensable for female fertility. KO mice with no phenotypic data are seldom published, but we believe that this information must be shared to prevent unnecessary experimentation by other laboratories.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice

Abbasi

Kodani

Emori

et al. 2020

Cells

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“…In our mouse model, the same experimental setting was used as in our previous work [34], which is described briefly in each chapter below.…”

Section: Methodsmentioning

confidence: 99%

“…Following our protocol, described previously [34], after the mice were killed, the abdomen was carefully opened and access to the uterus was gained by pushing intestinal tissue to the side. The uterus was then removed by surgical cuts at the cervix and the ovaries.…”

Section: Methodsmentioning

confidence: 99%

“…Endometrial mononuclear cells from the nonpregnant endometrium were isolated as described previously [34]. Here, the uterus was removed from the abdominal cavity, sliced with scissors, and digested with type IV collagenase at 37°C for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…According to our previous protocol [34], the spleens were cut into small pieces by a sharp sterile surgical knife, immersed in 2 ml PBS, and pushed gently through a 70 μ m nylon cell strainer with a syringe plunger. After that, cells were washed in PBS.…”

Section: Methodsmentioning

confidence: 99%

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TIM-3 and TIM-1 Could Regulate DecidualγδTCR Bright T Cells during Murine Pregnancy

Nörenberg

Meggyes

Jaksó

et al. 2019

Journal of Immunology Research

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Pregnancy is an immunological enigma where paternal antigens are present at the fetomaternal interface. What regulates the local immunotolerance, which is necessary to prevent rejection of the conceptus, is still under strong investigation. Gamma/delta T cells are believed to play a role in the local regulation of this immunotolerance towards the semiallogenic fetus. Gamma/delta T cells from the uterus and spleen of pregnant and nonpregnant mice were analyzed by flow cytometry. We confirmed that the rate of γδT cells in the decidua increases during murine pregnancy and half of decidual γδT cells are CD4+. Furthermore, we found a unique association of CD4 or CD8 coreceptor expression with their γδTCR intensity, where in all investigated groups CD4- or CD8-positive γδT cells seemed principally to be γδTCRdim. In addition, compared to peripheral γδT lymphocytes, a greater proportion of decidual γδT cells expressed the cytotoxic marker CD107a and markers of Th1 or Th2 polarization (TIM-3, TIM-1), where decidual γδTCRbright cells were characterized by high TIM-3 and TIM-1 receptor expression. On the other hand, no difference in the expression of CD160, a receptor with dual function affecting cytotoxicity and T cell inhibition, was detected. Within lymphocytes expressing CD107a, TIM-1, or CD160, the rate of γδT cells was significantly higher in the decidua. According to our results, cytotoxic potential of decidual γδTCRbright cells could be regulated by TIM-3 ligation, while the TIM-1 receptor seems to be able to influence the Th1-Th2 balance at the fetomaternal interface. These mechanisms could play a part in the active maternal immunotolerance towards the fetus, allowing an efficient protection against pathogens during healthy murine pregnancy.

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Roles of γδT cells in pregnancy and pregnancy‐related complications

Hong

Wang

et al. 2021

American J Rep Immunol

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A successful pregnancy is a complex and unique process comprised of discrete events, including embryo implantation, placentation, and parturition. To maintain the balance between maternal‐fetal immune tolerance and resistance to infections, the maternal immune system must have a high degree of stage‐dependent plasticity throughout the period of pregnancy. Innate immunity is the frontline force for the establishment of early anti‐infection and tolerance mechanisms in mammals. Belonging to the innate immune system, a subset of T cells called γδT cells (based on γδT cell receptors) are the main participants in immune surveillance and immune defense. Unlike traditional αβT cells, γδT cells are regarded as a bridge between innate immunity and acquired immunity. In this review, we summarize current knowledge on the functional plasticity of γδT cells during pregnancy. Furthermore, we discuss the roles of γδT cells in pathological pregnancies.

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Expansion of CD4 phenotype among CD160 receptor‐expressing lymphocytes in murine pregnancy

Abstract: The expansion of CD4-expressing CD160 decidual lymphocytes is a new observation suggesting a potential regulatory role of T-cell function during mouse pregnancy. The altered immunological character of CD160 lymphocytes could play a role in the maintenance of murine pregnancy.

Cited by 4 publications

References 32 publications

CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice

CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice

TIM-3 and TIM-1 Could Regulate DecidualγδTCR Bright T Cells during Murine Pregnancy

Roles of γδT cells in pregnancy and pregnancy‐related complications

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