Expansion of cord blood stem cells in fibronectin-coated microfluidic bioreactor

Moghadasi, Mohamad Hossein; Hajifathali, Abbas; Azad, Mehdi; Rahmani, Amir M.; Soleimani, Masoud

doi:10.1016/j.htct.2021.06.011

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…The upregulation of the CXCR 4 gene in CD133 + cells in microfluidic cultured cells (8.9%) had confirmed their homing ability and haematopoiesis reviving potential. 110 Therefore, from this study it can be understood that the bioreactor culture conditions are important for cell differentiation and maturation in a scaffold free microenvironment also.…”

Section: Microfluidic Bioreactors On Tissue Engineering Scaffoldsmentioning

confidence: 90%

Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

Thangadurai,

Srinivasan,

Sekar

et al. 2024

J. Mater. Chem. B

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Section: Microfluidic Bioreactors On Tissue Engineering Scaffoldsmentioning

confidence: 90%

Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

Thangadurai,

Srinivasan,

Sekar

et al. 2024

J. Mater. Chem. B

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“…The findings indicated poor cell adhesion that corresponded with a lack of the promoting effects of the hydrogel in the SS and a lack of ECM and adhesion molecules of the encapsulated cells in the SS [70,71]. This could be because ECM and adhesion molecules, such as fibronectin, ITG-αVβ3, and ITGβ1, are important molecules regulating the stemness, adhesion, and osteogenic differentiation of MSCs on the three-dimensional structure of contact-dependent MSCs [60,71,72]. As it has been reported that surface coating and cell adhesion are important regulating factors for successful cell culture in serum-free cell culture [70,73,74], the limited cell spreading and low levels of adhesion molecules of the encapsulated cells in SS possibly contributed to the low osteogenic differentiation potential of the encapsulated cells in the SS.…”

Section: Discussionmentioning

confidence: 99%

Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation

Arpornmaeklong,

Boonyuen,

Apinyauppatham

et al. 2024

Bioengineering

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Introduction: To develop a stem cell delivery model and improve the safety of stem cell transplantation for bone regeneration, this study aimed to determine the effects of stem cell sources, serum-free cell culture, and hydrogel cell encapsulation on the growth and osteogenic differentiation of mesenchymal stem cells (MSCs) from the oral cavity. Methods: The study groups were categorized according to stem cell sources into buccal fat pad adipose (hBFP-ADSCs) (Groups 1, 4, and 7), periodontal ligament (hPDLSCs) (Groups 2, 5, and 8), and dental pulp-derived stem cells (hDPSCs) (Groups 3, 6, and 9). MSCs from each source were isolated and expanded in three types of sera: fetal bovine serum (FBS) (Groups 1–3), human serum (HS) (Groups 4–6), and synthetic serum (SS) (StemPro™ MSC SFM) (Groups 7–9) for monolayer (m) and hydrogel cell encapsulation cultures (e). Following this, the morphology, expression of MSC cell surface antigens, growth, and osteogenic differentiation potential of the MSCs, and the expression of adhesion molecules were analyzed and compared. Results: SS decreased variations in the morphology and expression levels of cell surface antigens of MSCs from three cell sources (Groups 7m–9m). The levels of osteoblastic differentiation of the hPDLSCs and hBFP-ADSCs were increased in SS (Groups 8m and 7m) and the cell encapsulation model (Groups 1e, 4e, 7e–9e), but the promoting effects of SS were decreased in a cell encapsulation model (Groups 7e–9e). The expression levels of the alpha v beta 3 (ITG-αVβ3) and beta 1 (ITG-β1) integrins in the encapsulated cells in FBS (Group 1e) were higher than those in the SS (Group 7e). Conclusions: Human PDLSCs and BFP-ADSCs were the optimum stem cell source for stem cell encapsulation by using nanohydroxyapatite–calcium carbonate microcapsule–chitosan/collagen hydrogel in serum-free conditions.

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“…Studies have shown that expansion efficiency can be improved by stromal cocultures, such as autologous umbilical vein endothelial cells (HUVECs) ( 27 ), human stromal placenta layer ( 28 ), and human mesenchymal stem cells (MSCs) ( 29 ). In addition, coating matrix can improve the in vitro expansion efficiency of HSPCs such as collagen I matrix ( 30 ) and fibronectin ( 31 , 32 ). Further, molecules for expanding HSPCs have gradually been tapped, including the bioactive peptide SL-13R ( 33 ), the activator of Wnt signaling 6-bromoindirubin-3'-oxime (BIO) ( 34 ), and Levistilide A ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34+ hematopoietic stem/progenitor cells

Hua¹,

Jiao²,

Qiao³

et al. 2023

Ann Transl Med

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Background We investigated the effect of human serum albumin (HSA) on human umbilical cord blood (UCB) CD34 + hematopoietic stem/progenitor cells (HSPCs) cultured in vitro and transplanted in vivo . Methods Human umbilical cord blood mononuclear cells were obtained by density gradient centrifugation. CD34 + cells were then sorted by CD34 conjugated magnetic microbeads. The sorted cells were cultured with or without HSA for 8 days in vitro . After 8 days, all cells were harvested for flow phenotyping and colony formation cell (CFC) experiments. The cells were injected into immunodeficient mice (NOD/Shi-scid/IL2Rγnull, NOG) via intravenous injections. From 4 weeks post-transplantation, flow cytometry was used to calculate human cell chimerism in the peripheral blood (PB) every 2 weeks. Flow phenotyping of human cell chimerism in bone marrow and spleen was calculated 16 weeks post-transplantation. Results Compared to the control group, CD34 + cells cultured with HSA increased significantly in vitro . The long-term engraftment of HSPCs and the hematopoietic multilineage reconstruction capacity were preserved by HSA. Normal engraftment of human cells could be maintained via HSA treatment could maintain normal engraftment of human cells in recipient PB. Conclusions Here, we found that HSA was beneficial to maintaining CD34 + cell expansion and short-term colony formation in vitro and optimizing multilineage reconstitution in immunodeficient mice in vivo .

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Expansion of cord blood stem cells in fibronectin-coated microfluidic bioreactor

Cited by 3 publications

References 26 publications

Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs

Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34+ hematopoietic stem/progenitor cells

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