Expansion of CD56+ NK T and γδ T cells from cord blood of human neonates

Musha, Nobuyuki; Yoshida, Y; Sugahara, Satoshi; Yamagiwa, Satoshi; Koya, Toshiyuki; Watanabe, Hirotaka; Hatakeyama, Katsuyoshi; Abo, Toru

doi:10.1046/j.1365-2249.1998.00645.x

Cited by 47 publications

(29 citation statements)

References 38 publications

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“…Perforin expression is mainly confined to NK cells, 6 CD8 ϩ T cells, 7 CD56 ϩ T cells, 8 ␥␦ T cells 9 and activated CD4 cells. 10,11 In our study, we measured NK cells, CD8 ϩ T cells, and CD56 ϩ T cells.…”

Section: Discussionmentioning

confidence: 99%

Perforin expression in cytotoxic lymphocytes from patients with hemophagocytic lymphohistiocytosis and their family members

Kogawa

Lee

Villanueva

et al. 2002

Blood

227

177

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Section: Discussionmentioning

confidence: 99%

Perforin expression in cytotoxic lymphocytes from patients with hemophagocytic lymphohistiocytosis and their family members

Kogawa

Lee

Villanueva

et al. 2002

Blood

227

177

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“…A recent study reported "leakage" of T cell progenitors from the thymus to the gut as a novel mechanism for thymus-independent T cell selection (52). More specifically, Gunther et al (53) reported CD56 expression on CD7 ϩ /CD3 Ϫ T cell progenitors in the gut that are capable of differentiating into mature T cells, and Musha et al (54) proposed extrathymic development of CD56 ϩ T cells in studies of human neonate cord blood. NK markers such as CD56 or CD161 are expressed early in lymphoid cell development before the T-NK split, but lost during T cell selection and maturation in the thymus.…”

Section: Cd56mentioning

confidence: 99%

CD56 Marks an Effector T Cell Subset in the Human Intestine

Cohavy

Targan

2007

The Journal of Immunology

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T cells are key mediators of intestinal immunity, and specific T cell subsets can have differing immunoregulatory roles in animal models of mucosal inflammation. In this study, we describe human CD56+ T cells as a morphologically distinct population expressing a mature, nonproliferative phenotype that is frequent in the gut. Enhanced potential for IFN-γ and TNF synthesis suggested a proinflammatory function, and we directly demonstrate effector function mediated by direct T-T interaction with responder cells in vitro. CD56+ T cells from peripheral blood responded to the gut-related CD2 signal, and were necessary for effective CD2-mediated proliferation of peripheral blood CD56− T cells. Our findings associate CD56+ T cells with the intestinal immune compartment and suggest a putative effector function in human mucosal immunity.

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“…Both cytotoxic activity and cytokine production of resting NK cells in cord blood are lower than those of adults 33,34 . Nevertheless, neonatal NK cells have the capability of regaining their function at the same or even greater level than that of adults by stimulation with IL-2 35,36 or IL-12 alone 37 , or IL-12 with IL-18 37 . Our finding that pre-eclampsia was associated with an increase in the proportion of NK cells in umbilical cord blood is in agreement with results reported by Darmochwal-Kolarz and colleagues 10 .…”

Section: Journal Of Maternal-fetal and Neonatal Medicinementioning

confidence: 99%

Neonates born to pre-eclamptic mothers have a higher percentage of natural killer cells (CD3 - /CD56+16+) in umbilical cord blood than those without pre-eclampsia

White

Ahlstrand²

2003

DJMF

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Objective: Maternal endothelial dysfunction and intravascular inflammation have been implicated in the mechanisms of disease responsible for the clinical syndrome of pre-eclampsia. Recently, the activation of the innate limb of the immune response (neutrophils and monocytes) in the fetal circulation has been reported in neonates born to mothers with pre-eclampsia. Natural killer (NK) cells are identified morphologically as a subpopulation of lymphocytes, but functionally as one component of the innate immune system. NK cells participate in the control of viral or bacterial infection, regulation of hematopoiesis, production of cytokines and cytotoxicity of neoplastic cells. Accumulating evidence suggests that the innate system is required for mounting an adequate adaptive response. NK cells, originally defined as effector cells of the innate immune system, may also play a role as regulatory cells for the adaptive immune system. This study was designed to determine the proportion of the NK cell subset of lymphocytes in umbilical cord blood of neonates born to mothers with and without pre-eclampsia. Methods: A cross-sectional study including neonates of mothers with (n = 48) and those without pre-eclampsia (control group) (n = 72) was conducted. Pre-eclampsia was diagnosed in the presence of hypertension and proteinuria. The control group consisted of neonates (premature and term) with no evidence of acute inflammation within the extraplacental membranes (chorioamnionitis). Umbilical cord blood was collected at the time of delivery, and assayed using monoclonal antibodies for selective cluster differentiation (CD) antigens in order to determine the proportion of NK cells as a percentage of total lymphocytes. The immunophenotypic characteristic was determined using flow cytometry, and NK cells were identified by positivity of CD16 and CD56 without CD3 (CD3-/CD56+16+). Log transformation of the percentage of NK cells was performed. Parametric statistics were used for analysis. Multiple regression analysis was utilized to examine the contribution of potentially confounding factors on the proportion of NK cells. A p value of < 0.05 was considered statistically significant. Results: Neonates born to mothers with pre-eclampsia had a significantly higher percentage of NK cells (CD3-/CD56+16+) than those in the control group (pre-eclampsia, mean ± SD 17 ± 9% vs. control, mean ± SD 12 ± 7.5%; p = 0.001). Multiple regression analysis suggested that umbilical cord blood pH of < 7.2, labor with vaginal delivery and maternal pre-eclampsia were associated with an increased percentage of NK cells in umbilical cord blood. Conclusions: Pre-eclampsia is associated with a higher NK cell (CD3-/CD56+16+) subset of lymphocytes in umbilical cord blood than in the control group. This difference cannot be explained by fetal acidosis or the presence of labor.

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Expansion of CD56+ NK T and γδ T cells from cord blood of human neonates

Cited by 47 publications

References 38 publications

Perforin expression in cytotoxic lymphocytes from patients with hemophagocytic lymphohistiocytosis and their family members

Perforin expression in cytotoxic lymphocytes from patients with hemophagocytic lymphohistiocytosis and their family members

CD56 Marks an Effector T Cell Subset in the Human Intestine

Neonates born to pre-eclamptic mothers have a higher percentage of natural killer cells (CD3 - /CD56+16+) in umbilical cord blood than those without pre-eclampsia

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